Chapter 11.6 : Detection of Antibodies

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Lyme disease, or Lyme borreliosis (LB), is a multisystem disease caused by and transmitted through the bite of infected ticks ( ). It is currently the most frequent vector- borne infectious disease in the United States, with the highest incidence in the northeastern and midwestern states ( ). LB is also prevalent in Europe, where evidence of the disease existed in the early 1900s and where it is transmitted by complex.

Citation: Garcia L. 2010. Detection of Antibodies, p 173-182. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch11.6
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23. Dressler, F.,, J. A. Whalen,, B. N. Reinhardt,, and A. C. Steere. 1993. Western blotting in the serodiagnosis of Lyme disease. J. Infect. Dis. 167: 392 400.
24. Engstrom, S. M.,, E. Shoop,, and R. C. Johnson. 1995. Immunoblot interpretation criteria for serodiagnosis of early Lyme disease. J. Clin. Microbiol. 33: 419 427.
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29. Gomes-Solecki, M. J. C.,, J. J. Dunn,, B. J. Luft,, J. Castillo,, D. E. Dykhuizen,, X. Yang,, J. D. Glass,, and R. J. Dattwyler. 2000. Recombinant chimeric borrelia proteins for diagnosis of Lyme disease. J. Clin. Microbiol. 38: 2530 2535.
30. Gomes-Solecki, M. J. C.,, L. Meirelles,, J. Glass,, and R. J. Dattwyler. 2007. Epitope length, genospecies dependency, and serum panel effect in the IR6 enzyme-linked immunosorbent assay for detection of antibodies to Borrelia burgdorferi. Clin. Vaccine Immunol. 14: 875 879.
31. Hauser, U.,, G. Lehnert,, and B. Wilske. 1999. Validity of interpretation criteria for standardized Western blots (immunoblots) for serodiagnosis of Lyme borreliosis based on sera collected throughout Europe. J. Clin. Microbiol. 37: 2241 2247.
32. Johnson, B. J.,, B. J. Biggerstaff,, R. M. Bacon,, and M. E. Schriefer. 2004. Cost-effectiveness of peptide-antigen immunoassays for Lyme disease. J. Infect. Dis. 189: 1962 1964.
33. Klempner, M. S.,, C. H. Schmid,, L. Hu,, A. C. Steere,, G. Johnson,, B. McCloud,, R. Noring,, and A. Weinstein. 2001. Intralaboratory reliability of serologic and urine testing for Lyme disease. Am. J. Med. 110: 217 219.
34. Lawrenz, M. B.,, J. M. Hardham,, R. T. Owens,, J. Nowakowski,, A. C. Steere,, G. P. Wormser,, and S. J. Norris. 1999. Human antibody responses to VlsE antigenic variation protein of Borrelia burgdorferi. J. Clin. Microbiol. 37: 3997 4004.
35. Ledue, T. B.,, M. F. Collins,, and W. Y. Craig. 1996. New laboratory guidelines for serologic diagnosis of Lyme disease: evaluation of the two-test protocol. J. Clin. Microbiol. 34: 2343 2350.
36. Liang, F. T.,, A. C. Steere,, A. R. Marques,, B. J. B. Johnson,, J. N. Miller,, and M. T. Philipp. 1999. Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of Borrelia burgdorferi VlsE. J. Clin. Microbiol. 37: 3990 3996.
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38. Marangoni, A.,, M. Sparacino,, and F. Cavrini. 2005. Comparative evaluation of three different ELISA methods for the diagnosis of early culture-confirmed Lyme disease in Italy. J. Med. Microbiol. 54: 361 367.
39. Massarotti, E. M.,, S. W. Luger,, D. W. Rahn,, R. P. Messner,, J. B. Wong,, R. C. Johnson,, and A. C. Steere. 1992. Treatment of early Lyme disease. Am. J. Med. 92: 396 403.
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41. Mogilyansky, E.,, C. C. Loa,, M. E. Adelson,, E. Mordechai,, and R. C. Tilton. 2004. Comparison of Western immunoblotting and the C6 Lyme antibody test for laboratory detection of Lyme disease. Clin. Diagn. Lab. Immunol. 11: 924 929.
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43. Nachamkin, I.,, D. L. Riddle,, M. Feldman,, and P. H. Edelstein. 1996. Utilization of tests for Lyme disease antibody at a university hospital. Clin. Diagn. Lab. Immunol. 3: 287 289.
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45. Nichol, G.,, D. T. Dennis,, A. C. Steere,, R. Lightfoot,, G. Wells,, B. Shea,, and P. Tugwell. 1998. Test-treatment strategies for patients suspected of having Lyme disease: a cost-effectiveness analysis. Ann. Intern. Med. 128: 37 48.
46. Nocton, J. J.,, F. Dressler,, B. J. Rutledge,, P. N. Rys,, D. H. Persing,, and A. C. Steere. 1994. Detection of Borrelia burgdorferi DNA by polymerase chain reaction in synovial fluid from patients with Lyme arthritis. N. Engl. J. Med. 330: 229 234.
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Table 11.6-1

Lyme disease stages

ACA, achrodermatitis chronica atrophicans.

Citation: Garcia L. 2010. Detection of Antibodies, p 173-182. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch11.6

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