Chapter 12.6 : Appendix 12.6-1—Unidirectional Work Flow and Minimization of Contamination

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Appendix 12.6-1—Unidirectional Work Flow and Minimization of Contamination, Page 1 of 1

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The extraordinary sensitivity of nucleic acid amplification methods makes them invaluable tools for detecting infectious agents. However, the ability to detect small quantities of nucleic acids also makes these techniques prone to false-positive results due to specimen-to-specimen cross-contamination or carryover contamination from previously amplified products. The risk of contamination can be greatly reduced by maintaining a unidirectional work flow and by strictly adhering to appropriate QC procedures.

Citation: Garcia L. 2010. Appendix 12.6-1—Unidirectional Work Flow and Minimization of Contamination, p 428-429. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch12.6
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1. College of American Pathologists. 2009. Laboratory Accreditation Program, Microbiology Checklist, rev. 06/15/2009. College of American Pathologists, Northfield, IL.
2. Health Protection Agency. 2006. Good laboratory practice when performing molecular amplification assays. National Standard Method QSOP 38, issue 3. http://www.hpa-standardmethods.org.uk/pdf_sops.asp..
3. Mitchell, P. S.,, J. J. Germer,, and R. Patel,. 2004. Nucleic acid amplification methods: laboratory design and operations, p. 85 93. In D. H. Persing,, F. C. Tenover,, Y.-W. Tang,, E. R. Unger,, D. A. Relman,, and T. J. White (ed.), Molecular Microbiology: Diagnostic Principles and Practice. ASM Press, Washington, DC.
4. Nolte, F. S.,, F. R. Cockerill,, P. J. Dailey,, D. Hillyard,, S. McDonough,, R. F. Meyer,, and R. G. Shively. 2006. Clinical and Laboratory Standards Institute (CLSI). Molecular Diagnostic Methods for Infectious Diseases; Approved Guideline—Second Edition. Clinical and Laboratory Standards Institute, Wayne, PA.
5. U.S. Environmental Protection Agency. 2004. Quality Assurance/Quality Control Guidance for Laboratories Performing PCR Analyses on Environmental Samples. U.S. Environmental Protection Agency, Cincinnati, OH. http://www.epa.gov/ nerlcwww/qa_qc_pcr10_04.pdf.

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