Chapter 13.14 : Microbiological Assessment of Orthopedic Surgery Sites

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The severe morbidity associated with infection following implantation of orthopedic prosthetic devices or repair of bone fractures at septic trauma sites requires early and accurate microbiological assessment of these sites for successful management. Although no procedural or interpretive standards for culture of these sites have been established, the following is a set of procedures often used by surgical teams to assess microbial load or, along with other laboratory findings, to confirm the presence of an infection. These procedures include (i) enumeration of bacteria in tissue from a contaminated trauma site prior to repair of fracture, (ii) intraoperative culture of a site selected for implantation of a prosthesis, (iii) culture of fluid removed from the joint, and (iv) intraoperative culture of a failed prosthesis site.

Citation: Garcia L. 2010. Microbiological Assessment of Orthopedic Surgery Sites, p 512-518. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch13.14
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1. Atkins, B. L.,, N. Athanasou,, J. J. Deeks,, D. W. M. Crook,, H. Simpson,, T. E. A. Peto,, P. McLardy-Smith,, A. R. Berendt, and The OSIRIS Collaborative Study Group. 1998. Prospective evaluation of criteria for microbiological diagnosis of prosthetic-joint infection at revision arthroplasty. J. Clin. Microbiol. 36: 2932 2939.
2. Cooney, W. P., III,, R. H. Fitzgerald, Jr.,, J. H. Dobyns,, and J. A. Washington II. 1982. Quantitative wound cultures in upper extremity trauma. J. Trauma 33: 112 117.
3. Cuckler, J. M.,, A. M. Star,, A. Alavi,, and R. B. Noto. 1991. Diagnosis and management of the infected total joint arthroplasty. Orthop. Clin. N. Am. 22: 523 530.
4. Trampuz, A.,, K. E. Piper,, M. J. Jacobson,, A. D. Hanssen,, K. K. Unni,, D. R. Osmon,, J. N. Mandrekar,, F. R. Cockerill,, J. M. Steckelberg,, J. F. Greenleaf,, and R. Patel. 2007. Sonication of removed hip and knee prostheses for diagnosis of infection. N. Engl. J. Med. 357: 654 663.
5. Atkins, B. L.,, N. Athanasou,, H. Simpson,, P. McLardy-Smith,, D. W. M. Crook,, A. R. Brenedt, and The OSIRIS Collaborative Study Group Oxford. 1997. Is the Gram stain useful in diagnosing infection at elective revision arthroplasty? J. Infect. 34: 75.
6. Atkins, B. L.,, and I. C. Bowler. 1998. The diagnosis of large joint sepsis. J. Hosp. Infect. 40: 263 274.
7. Duff, G. P.,, P. F. Lachiewicz,, and S. S. Kelley. 1996. Aspiration of the knee joint before revision arthroplasty. Clin. Orthop. 331: 132 139.
8. Gruninger, R. P., 1989. Diagnostic microbiology in bone and joint infections, p. 42 51. In R. B. Gustilo,, R. P. Gruninger,, and D. T. Tsukayama (ed.), Orthopaedic Infection: Diagnosis and Treatment. W. B. Saunders Co., Philadelphia, PA.
9. Kortekangas, P.,, H. T. Aro,, and O. P. Lehtonen. 1995. Synovial fluid culture and blood culture in acute arthritis. A multi-case report of 90 patients. Scand. J. Rheumatol. 24: 44 47.
10. Steckelberg, J. M.,, and D. R. Osmon,. 1994. Prosthetic joint infections, p. 259 290. In A. L. Bisno, and F. A. Waldvogel (ed.), Infections Associated with Indwelling Medical Devices, 2nd ed. American Society for Microbiology, Washington, DC.

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