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Chapter 3.16 : Guidelines for Biochemical Identification of Aerobic Bacteria
Editor:
Lynne S. Garcia1
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Affiliations:
1: LSG & Associates, Santa Monica, California
Content Type: Reference
Publication Year:
2010
Category: Clinical Microbiology
Book DOI:
10.1128/9781555817435
Chapter DOI:
10.1128/9781555817435.ch3.16
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Guidelines for Biochemical Identification of Aerobic Bacteria, Page 1 of 2
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Abstract:
On the following pages are 48 biochemical procedures in alphabetical order, including both simple, rapid test procedures and standard conventional methods, for the identification of gram-positive and gram-negative bacteria. The biochemical tests have been selected that are most useful to laboratories, with emphasis on rapid testing. The list of tests includes the generally accepted tests that all laboratories should be able to perform to identify the clinically important microorganisms encountered in the laboratory, as well as some for use by reference or referral laboratories. Smaller laboratories may choose to perform fewer tests and refer cultures when less common microorganisms are found in culture. When identification to the species level is not clinically important, tests to separate these species are not included. When there is a choice of different tests that can be performed, both are presented and the user can choose which is preferable for use in the laboratory.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
References
/content/book/10.1128/9781555817435.chap3.16
Tables
Guidelines for Biochemical Identification of Aerobic Bacteria
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-1
Table 3.16-1a
Summary of bacterial identification systems
a Microbact 12A and 12B are in strip format; the 12E and 24E have microplate formats.
b RT, room temperature.
c Combination identification and susceptibility panels are also available: NMIC/ID and PMIC/ID for gram-negative and gram-positive organisms, respectively.
d See reference 3 for performance data for the new Vitek 2 ANC card.
10.1128/9781555817435/tab3-16-1a_thmb.gif
10.1128/9781555817435/tab3-16-1a.gif
Table 3.16-1a
Summary of bacterial identification systems
a Microbact 12A and 12B are in strip format; the 12E and 24E have microplate formats.
b RT, room temperature.
c Combination identification and susceptibility panels are also available: NMIC/ID and PMIC/ID for gram-negative and gram-positive organisms, respectively.
d See reference 3 for performance data for the new Vitek 2 ANC card.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-1b
Table 3.16-1b
Summary of bacterial identification systems
a Microbact 12A and 12B are in strip format; the 12E and 24E have microplate formats.
b RT, room temperature.
c Combination identification and susceptibility panels are also available: NMIC/ID and PMIC/ID for gram-negative and gram-positive organisms, respectively.
d See reference 3 for performance data for the new Vitek 2 ANC card.
10.1128/9781555817435/tab3-16-1b_thmb.gif
10.1128/9781555817435/tab3-16-1b.gif
Table 3.16-1b
Summary of bacterial identification systems
a Microbact 12A and 12B are in strip format; the 12E and 24E have microplate formats.
b RT, room temperature.
c Combination identification and susceptibility panels are also available: NMIC/ID and PMIC/ID for gram-negative and gram-positive organisms, respectively.
d See reference 3 for performance data for the new Vitek 2 ANC card.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-1c
Table 3.16-1c
Summary of bacterial identification systems
a Microbact 12A and 12B are in strip format; the 12E and 24E have microplate formats.
b RT, room temperature.
c Combination identification and susceptibility panels are also available: NMIC/ID and PMIC/ID for gram-negative and gram-positive organisms, respectively.
d See reference 3 for performance data for the new Vitek 2 ANC card.
10.1128/9781555817435/tab3-16-1c_thmb.gif
10.1128/9781555817435/tab3-16-1c.gif
Table 3.16-1c
Summary of bacterial identification systems
a Microbact 12A and 12B are in strip format; the 12E and 24E have microplate formats.
b RT, room temperature.
c Combination identification and susceptibility panels are also available: NMIC/ID and PMIC/ID for gram-negative and gram-positive organisms, respectively.
d See reference 3 for performance data for the new Vitek 2 ANC card.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-2a
Table 3.16-2a-a
Database entries of the Enterobacteriaceae (human isolates) for manual systems a
a Reproduced from reference 2 .
b Some products give a choice between two species; the alternate species is indicated in parentheses.
c #, genus-level identification only.
d Previous taxonomic designation.
e Able to differentiate O111 from O157.
f Also contains 10 unnamed enteric groups in the database.
g “Group” indicates Yersinia group.
10.1128/9781555817435/tab3-16-2a-a_thmb.gif
10.1128/9781555817435/tab3-16-2a-a.gif
Table 3.16-2a-a
Database entries of the Enterobacteriaceae (human isolates) for manual systems a
a Reproduced from reference 2 .
b Some products give a choice between two species; the alternate species is indicated in parentheses.
c #, genus-level identification only.
d Previous taxonomic designation.
e Able to differentiate O111 from O157.
f Also contains 10 unnamed enteric groups in the database.
g “Group” indicates Yersinia group.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-2ab
Table 3.16-2a-b
Database entries of the Enterobacteriaceae (human isolates) for manual systems a
a Reproduced from reference 2 .
b Some products give a choice between two species; the alternate species is indicated in parentheses.
c #, genus-level identification only.
d Previous taxonomic designation.
e Able to differentiate O111 from O157.
f Also contains 10 unnamed enteric groups in the database.
g “Group” indicates Yersinia group.
10.1128/9781555817435/tab3-16-2a-b_thmb.gif
10.1128/9781555817435/tab3-16-2a-b.gif
Table 3.16-2a-b
Database entries of the Enterobacteriaceae (human isolates) for manual systems a
a Reproduced from reference 2 .
b Some products give a choice between two species; the alternate species is indicated in parentheses.
c #, genus-level identification only.
d Previous taxonomic designation.
e Able to differentiate O111 from O157.
f Also contains 10 unnamed enteric groups in the database.
g “Group” indicates Yersinia group.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-2b-a
Table 3.16-2b-a
Database entries of the Enterobacteriaceae (human isolates) for automated systems a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is in parentheses.
b Includes the ability to differentiate between serogroups O111 and O157
c #, genus identification only.
10.1128/9781555817435/tab3-16-2b-a_thmb.gif
10.1128/9781555817435/tab3-16-2b-a.gif
Table 3.16-2b-a
Database entries of the Enterobacteriaceae (human isolates) for automated systems a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is in parentheses.
b Includes the ability to differentiate between serogroups O111 and O157
c #, genus identification only.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-2b-b
Table 3.16-2b-b
Database entries of the Enterobacteriaceae (human isolates) for automated systems a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is in parentheses.
b Includes the ability to differentiate between serogroups O111 and O157
c #, genus identification only.
10.1128/9781555817435/tab3-16-2b-b_thmb.gif
10.1128/9781555817435/tab3-16-2b-b.gif
Table 3.16-2b-b
Database entries of the Enterobacteriaceae (human isolates) for automated systems a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is in parentheses.
b Includes the ability to differentiate between serogroups O111 and O157
c #, genus identification only.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-3
Table 3.16-3a
Database entries of the gram-positive organisms (human isolates) for bioMérieux and Dade MicroSan products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is in parentheses.
b Includes the ability to differentiate between serogroups O111 and O157
c #, genus identification only.
10.1128/9781555817435/tab3-16-3a_thmb.gif
10.1128/9781555817435/tab3-16-3a.gif
Table 3.16-3a
Database entries of the gram-positive organisms (human isolates) for bioMérieux and Dade MicroSan products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is in parentheses.
b Includes the ability to differentiate between serogroups O111 and O157
c #, genus identification only.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-3b
Table 3.16-3b
Database entries of the gram-positive organisms (human isolates) for bioMérieux and Dade MicroSan products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is in parentheses.
b Includes the ability to differentiate between serogroups O111 and O157
c #, genus identification only.
10.1128/9781555817435/tab3-16-3b_thmb.gif
10.1128/9781555817435/tab3-16-3b.gif
Table 3.16-3b
Database entries of the gram-positive organisms (human isolates) for bioMérieux and Dade MicroSan products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is in parentheses.
b Includes the ability to differentiate between serogroups O111 and O157
c #, genus identification only.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-3c
Table 3.16-3c
Database entries of the gram-positive organisms (human isolates) for bioMérieux and Dade MicroSan products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is in parentheses.
b Includes the ability to differentiate between serogroups O111 and O157
c #, genus identification only.
10.1128/9781555817435/tab3-16-3c_thmb.gif
10.1128/9781555817435/tab3-16-3c.gif
Table 3.16-3c
Database entries of the gram-positive organisms (human isolates) for bioMérieux and Dade MicroSan products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is in parentheses.
b Includes the ability to differentiate between serogroups O111 and O157
c #, genus identification only.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-4
Table 3.16-4a
Databaseentriesofthegram-positive organisms for BD Diagnostics, Remel, TREK, Biolog, and MIDI products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is indicated in parentheses.
b Oxoid has a similar product for Listeria identification called the Listeria Identification System 12 S. It identifies six species.
c #, genus-level identification only.
10.1128/9781555817435/tab3-16-4a_thmb.gif
10.1128/9781555817435/tab3-16-4a.gif
Table 3.16-4a
Databaseentriesofthegram-positive organisms for BD Diagnostics, Remel, TREK, Biolog, and MIDI products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is indicated in parentheses.
b Oxoid has a similar product for Listeria identification called the Listeria Identification System 12 S. It identifies six species.
c #, genus-level identification only.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-4b
Table 3.16-4b
Databaseentriesofthegram-positive organisms for BD Diagnostics, Remel, TREK, Biolog, and MIDI products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is indicated in parentheses.
b Oxoid has a similar product for Listeria identification called the Listeria Identification System 12 S. It identifies six species.
c #, genus-level identification only.
10.1128/9781555817435/tab3-16-4b_thmb.gif
10.1128/9781555817435/tab3-16-4b.gif
Table 3.16-4b
Databaseentriesofthegram-positive organisms for BD Diagnostics, Remel, TREK, Biolog, and MIDI products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is indicated in parentheses.
b Oxoid has a similar product for Listeria identification called the Listeria Identification System 12 S. It identifies six species.
c #, genus-level identification only.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16-4c
Table 3.16-4c
Databaseentriesofthegram-positive organisms for BD Diagnostics, Remel, TREK, Biolog, and MIDI products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is indicated in parentheses.
b Oxoid has a similar product for Listeria identification called the Listeria Identification System 12 S. It identifies six species.
c #, genus-level identification only.
10.1128/9781555817435/tab3-16-4c_thmb.gif
10.1128/9781555817435/tab3-16-4c.gif
Table 3.16-4c
Databaseentriesofthegram-positive organisms for BD Diagnostics, Remel, TREK, Biolog, and MIDI products a
a Reproduced from reference 2 . Some products give a choice between two species; the alternate species is indicated in parentheses.
b Oxoid has a similar product for Listeria identification called the Listeria Identification System 12 S. It identifies six species.
c #, genus-level identification only.
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16
/content/10.1128/9781555817435.chap3.16.tab3.16.16
Untitled
10.1128/9781555817435/tab3-16-16_thmb.gif
10.1128/9781555817435/tab3-16-16.gif
Untitled
Citation:
Garcia L.
2010.
Guidelines for Biochemical Identification of Aerobic Bacteria,
p 487-502.
In
Clinical Microbiology Procedures Handbook, 3rd Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555817435.ch3.16