Chapter 3.5 : Body Fluid Cultures (Excluding Blood, Cerebrospinal Fluid, and Urine)

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Infection of normally sterile body fluids often results in severe morbidity and mortality; therefore, rapid and accurate microbiological assessment of these samples is important to successful patient management. Most organisms infecting these sites are not difficult to culture, but determining the significance of low numbers of commensal cutaneous microorganisms does present a challenge ( ). With the increased use of prostheses, immunosuppressive therapeutic regimens, and long-term care of individuals with chronic debilitating disease, the likelihood of true infection with commensal organisms has increased, making accurate diagnoses difficult. Care must be taken during specimen collection and transport to ensure that the specimen is not contaminated. Any microorganism found in a normally sterile site must be considered significant, and all isolates must be reported.

Citation: Garcia L. 2010. Body Fluid Cultures (Excluding Blood, Cerebrospinal Fluid, and Urine), p 183-191. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.5
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1. Alfa, M. J.,, P. Degagne,, N. Olson,, and G. K. M. Harding. 1997. Improved detection of bacterial growth in continuous ambulatory peritoneal dialysis effluent by the use of BacT/Alert FAN bottles. J. Clin. Microbiol. 35: 862 866.
2. Atkins, B. L.,, N. Athanasou,, J. J. Deeks,, D. W. M. Crook,, H. Simpson,, T. E. A. Peto,, P. Mclardy-Smith,, A. R. Berendt, and the Osiris Collaborative Study Group. 1998. Prospective evaluation of criteria for microbiological diagnosis of prosthetic-joint infection at revision arthroplasty. J. Clin. Microbiol. 36: 2932 2939.
3. Bernard, L.,, B. Pron,, A. Vuagnat,, V. Gleizes,, F. Signoret,, P. Denormandie,, A. Si-Ali,, C. Perrone,, J. M. Feron,, J. L. Gaillard, and the Groupe d'Etude sur l'Ostéite. 2002. The value of suction drainage fluid culture during aseptic and septic orthopedic surgery: a prospective study of 901 patients. Clin. Infect. Dis. 34: 46 49.
4. Blondeau, J. M.,, G. B. Pylypchuk,, J. E. Kappel,, R. B. Baltzan,, Y. Yaschuk,, and A. J. Adolph. 1995. Evaluation of aerobic Bactec 6A non-resin- and 16A resin-containing media for the recovery of microorganisms causing peritonitis. Diagn. Microbiol. Infect. Dis. 22: 361 368.
5. Bourbeau, P.,, J. Riley,, B. J. Heiter,, R. Master,, C. Young,, and C. Pierson. 1998. Use of the BacT/Alert blood culture system for culture of sterile body fluids other than blood. J. Clin. Microbiol. 36: 3273 3277.
6. Brook, I.,, and E. H. Frazier. 1993. Anaerobic osteomyelitis and arthritis in a military hospital: a 10-year experience. Am. J. Med. 94: 21 28.
7.Clinical and Laboratory Standards Institute. 2008. Performance Standards for Anti-microbial Susceptibility Testing. Eighteenth informational supplement. Approved standard M100-S18. C linical and Laboratory Standards Institute, Wayne, PA.
8.Clinical and Laboratory Standards Institute. 2004. Quality Assurance for Commercially Prepared Microbiological Culture Media, 3rd ed. Approved standard M22-A3. Clinical and Laboratory Standards Institute, Wayne, PA.
9. Everts, R. J.,, J. P. Heneghan,, P. O. Adholla,, and L. B. Reller. 2001. Validity of cultures of fluid collected through drainage catheters versus those obtained by direct aspiration. J. Clin. Microbiol. 39: 66 68.
10. Fuller, D. D.,, T. E. Davis,, P. C. Kibsey,, L. Rosmus,, L. W. Ayers,, M. Ott,, M. A. Saubolle,, and D. L. Sewell. 1994. Comparison of BACTEC Plus 26 and 27 media with and without fastidious organism supplement with conventional methods for culture of sterile body fluids. J. Clin. Microbiol. 32: 1488 1491.
11. Goldenberger, D.,, A. Kunzli,, P. Vogt,, R. Zbinden,, and M. Altwegg. 1997. Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing. J. Clin. Microbiol. 35: 4759 4764.
12. Harris, K. A.,, and J. C. Hartley. 2003. Development of broad-range 16S rRNA PCR for use in the routine diagnostic clinical microbiology service. J. Med. Microbiol. 52: 685 691.
13. Lejbkowicz, F.,, L. Cohn,, N. Hashman,, and I. Kassis. 1999. Recovery of Kingella kingae from blood and synovial fluid of two pediatric patients by using the BacT/Alert system. J. Clin. Microbiol. 37: 878. (Letter.)
14. Morris, A. J.,, S. J. Wilson,, C. E. Marx,, M. L. Wilson,, S. Mirrett,, and L. B. Reller. 1995. Clinical impact of bacteria and fungi recovered only from broth cultures. J. Clin. Microbiol. 33: 161 165.
15. Nakata, M.M.,, and R. P. Lewis. 1984. Anaerobic bacteria in bone and joint infections. Rev. Infect. Dis. 6( Suppl. 1): S165 S170.
16. Rantakokko-Jalava, K.,, S. Nikkari,, J. Jalava,, E. Eerola,, M. Skurnik,, O. Meurman,, O. Ruuskanen,, A. Alanen,, E. Kotilainen,, P. Toivanen,, and P. Kotilainen. 2000. Direct amplification of rRNA genes in diagnosis of bacterial infection. J. Clin. Microbiol. 38: 32 39.
17. Rinehold, C. E.,, D. J. Nickolai,, T. E. Piccinni,, B. A. Byford,, M. K. York,, and G. F. Brooks. 1988. Evaluation of broth media for routine culture of cerebrospinal and joint fluid specimens. Am. J. Clin. Pathol. 89: 671 674.
18. Runyon, B. A.,, M. R. Antillon,, E. A. Akriviadis,, and J. G. McHutchison. 1990. Bedside inoculation of blood culture bottles with ascitic fluid is superior to delayed inoculation in the detection of spontaneous bacterial peritonitis. J. Clin. Microbiol. 28: 2811 2812.
19. Scythes, K. D.,, M. Louis,, and A. E. Simor. 1996. Evaluation of nutritive capacities of 10 broth media. J. Clin. Microbiol. 34: 1804 1807.
20. Shanholtzer, C. J.,, P. J. Schaper,, and L. R. Peterson. 1982. Concentrated Gram stain smears prepared with a cytospin centrifuge. J. Clin. Microbiol. 16: 1052 1056.
21. Siersema, P. D.,, S. de Marie,, J. H. van Zeijl,, D. J. Bac,, and J. H. Wilson. 1992. Blood culture bottles are superior to lysis-centrifugation tubes for bacteriological diagnosis of spontaneous bacterial peritonitis. J. Clin. Microbiol. 30: 667 669.
22. von Essen, R.,, and A. Holtta. 1986. Improved method of isolating bacteria from joint fluids by the use of blood culture bottles. Ann. Rheum. Dis. 45: 454 457.
23. von Graevenitz, A.,, and D. Amsterdam. 1992. Microbiological aspects of peritonitis associated with continuous ambulatory peritoneal dialysis. Clin. Microbiol. Rev. 5: 36 48.
24. Yagupsky, P. 1999. Use of blood culture systems for isolation of Kingella kingae from synovial fluid. J. Clin. Microbiol. 37: 3785. (Letter.)
25. Yagupsky, P.,, N. Peled,, and J. Press. 2001. Use of BACTEC 9240 blood culture system for detection of Brucella melitensis in synovial fluid. J. Clin. Microbiol. 39: 738 739.
26. Yagupsky, P.,, and J. Press. 1997. Use of the Isolator 1.5 microbial tube for culture of synovial fluid from patients with septic arthritis. J. Clin. Microbiol. 35: 2410 2412.
27. Zucol, F.,, R. A. Ammann,, C. Berger,, C. Aebi,, M. Altwegg,, F. K. Niggli,, and D. Nadal. 2006. Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification. J. Clin. Microbiol. 44: 2750 2759.
28. Smith, J. W.,, and E. A. Pierey. 1995. Infectious arthritis. Clin. Infect. Dis. 20: 225 231.


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Table 3.5-1

Types of body fluids submitted for culture

Citation: Garcia L. 2010. Body Fluid Cultures (Excluding Blood, Cerebrospinal Fluid, and Urine), p 183-191. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.5
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Table 3.5-2

Common drainage tubes in clinical use

Citation: Garcia L. 2010. Body Fluid Cultures (Excluding Blood, Cerebrospinal Fluid, and Urine), p 183-191. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.5

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