Chapter 4.5 : Incubation Techniques for Anaerobic Bacteriology Specimens

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This procedure describes the various environmental methods and incubation conditions used for anaerobic bacteriology specimens once a properly selected, collected, and transported specimen arrives in the laboratory. Ideally, the specimen is processed immediately on arrival in the laboratory and incubated under anaerobic conditions to prevent further exposure to oxygen. Refer to procedure 4.2 for collection procedures, to procedure 4.3 for an-aerobic media, and to procedure 4.4 for processing and inoculation techniques.

Citation: Garcia L. 2010. Incubation Techniques for Anaerobic Bacteriology Specimens, p 709-712. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.5
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1. Engelkirk, P. G.,, J. Duben-Engelkirk,, and V. R. Dowell, Jr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
2. Forbes, B. A.,, D. F. Sahm,, and A. S. Weissfeld (ed.). 2007. Bailey and Scott's Diagnostic Bacte-riology, 12th ed. Mosby Elsevier, St. Louis, MO.
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4. Jousimies-Somer, H. R.,, P. Summanen,, D. M. Citron,, E. J. Baron,, H. M. Wexler,, and S. M. Finegold. 2002. Wadsworth Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
5. Shahin, M.,, W. Jamal,, T. Verghese,, and V. O. Rotimi. 2003. Comparative evaluation of anoxomat and conventional anaerobic GasPak jar systems for the isolation of anaerobic bacteria. Med. Princ. Pract. 12: 81 86.

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