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Chapter 5.2 : Broth Microdilution MIC Test

Editor: Lynne S. Garcia1
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Affiliations: 1: LSG & Associates, Santa Monica, California
Content Type: Reference
Publication Year: 2010

Category: Clinical Microbiology

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Broth Microdilution MIC Test, Page 1 of 2

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Abstract:

The broth microdilution MIC method is used to measure (semiquantitatively) the in vitro activity of an antimicrobial agent against a bacterial isolate. A sterile plastic tray containing various concentrations of antimicrobial agents is inoculated with a standardized number of test bacteria. After overnight incubation at 35°C, the MIC is determined by observing the lowest concentration of an antimicrobial agent which will inhibit visible growth of the bacterium. MICs obtained are interpreted as susceptible, intermediate, or resistant, based on the criteria listed in the NCCLS MIC standard ( ).

Citation: Garcia L. 2010. Broth Microdilution MIC Test, p 25-41. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.2
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References

/content/book/10.1128/9781555817435.chap5.2
1.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
2. Barry, A. L.,, and L. E. Braun. 1981. Reader error in determining minimal inhibitory concentrations with microdilution susceptibility test panels. J. Clin. Microbiol. 13: 228– 230.
3. Bradford, P. A. 2001. Extended-spectrum β-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin. Microbiol. Rev. 14: 933– 951.
4. Jorgensen, J. H., 2003. Susceptibility test methods: dilution and disk diffusion methods, p. 1108– 1127. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C.
5. Jorgensen, J. H.,, and M. J. Ferraro. 2000. Antimicrobial susceptibility testing: special needs for fastidious organisms and difficult-to-detect resistance mechanisms. Clin. Infect. Dis. 30: 799– 808.
1.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
1.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
1.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
1.NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.

Tables

Broth Microdilution MIC Test
Citation: Garcia L. 2010. Broth Microdilution MIC Test, p 25-41. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.2
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Citation: Garcia L. 2010. Broth Microdilution MIC Test, p 25-41. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.2
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Citation: Garcia L. 2010. Broth Microdilution MIC Test, p 25-41. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.2
/content/10.1128/9781555817435.chap5.2.tab1-app5.2-1
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Quick reference list for performing broth microdilution MIC tests

DCS, direct colony suspension standardized to a 0.5 McFarland turbidity standard; DCS/LPG, direct colony suspension or log-phase growth standardized to a 0.5 McFarland turbidity standard; HTM, test medium; LHB, lysed horse blood; GM, gentamicin; STR, streptomycin

All incubation temperatures are 35°C.

Tests for these species not fully addressed by the NCCLS; “presumptive” indicates that at least parts of the procedure are nonstandardized.

And other penicillinase-stable penicillins.

10.1128/9781555817435/tab1-app5.2-1_thmb.gif
10.1128/9781555817435/tab1-app5.2-1.gif
Generic image for table
Untitled

Quick reference list for performing broth microdilution MIC tests

DCS, direct colony suspension standardized to a 0.5 McFarland turbidity standard; DCS/LPG, direct colony suspension or log-phase growth standardized to a 0.5 McFarland turbidity standard; HTM, test medium; LHB, lysed horse blood; GM, gentamicin; STR, streptomycin

All incubation temperatures are 35°C.

Tests for these species not fully addressed by the NCCLS; “presumptive” indicates that at least parts of the procedure are nonstandardized.

And other penicillinase-stable penicillins.

Citation: Garcia L. 2010. Broth Microdilution MIC Test, p 25-41. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.2
/content/10.1128/9781555817435.chap5.2.tab2-pg5-2-12
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10.1128/9781555817435/tab2-pg5-2-12_thmb.gif
10.1128/9781555817435/tab2-pg5-2-12.gif
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Citation: Garcia L. 2010. Broth Microdilution MIC Test, p 25-41. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.2
/content/10.1128/9781555817435.chap5.2.tab2-pg5-2-14
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10.1128/9781555817435/tab2-pg5-2-14_thmb.gif
10.1128/9781555817435/tab2-pg5-2-14.gif
Generic image for table
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Citation: Garcia L. 2010. Broth Microdilution MIC Test, p 25-41. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.2
/content/10.1128/9781555817435.chap5.2.tab2-app5-2-7a

Broth Microdilution QC

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10.1128/9781555817435/tab2-app5-2-7a.gif
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Broth Microdilution QC

Citation: Garcia L. 2010. Broth Microdilution MIC Test, p 25-41. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.2
/content/10.1128/9781555817435.chap5.2.tab2-app5-2-7b
Untitled
10.1128/9781555817435/tab2-app5-2-7b_thmb.gif
10.1128/9781555817435/tab2-app5-2-7b.gif
Generic image for table
Untitled

Citation: Garcia L. 2010. Broth Microdilution MIC Test, p 25-41. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.2

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