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Broth Microdilution MIC Test, Page 1 of 2
< Previous page Next page > /docserver/preview/fulltext/10.1128/9781555817435/9781555815271_Chap5_2-1.gif /docserver/preview/fulltext/10.1128/9781555817435/9781555815271_Chap5_2-2.gifAbstract:
The broth microdilution MIC method is used to measure (semiquantitatively) the in vitro activity of an antimicrobial agent against a bacterial isolate. A sterile plastic tray containing various concentrations of antimicrobial agents is inoculated with a standardized number of test bacteria. After overnight incubation at 35°C, the MIC is determined by observing the lowest concentration of an antimicrobial agent which will inhibit visible growth of the bacterium. MICs obtained are interpreted as susceptible, intermediate, or resistant, based on the criteria listed in the NCCLS MIC standard ( 1 ).