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Chapter 5.6 : Agar Screen Test To Detect Vancomycin Resistance in spp.

Editor: Lynne S. Garcia1
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Affiliations: 1: LSG & Associates, Santa Monica, California
Content Type: Reference
Publication Year: 2010

Category: Clinical Microbiology

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Agar Screen Test To Detect Vancomycin Resistance in spp., Page 1 of 2

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Abstract:

The vancomycin agar screen is used to detect vancomycin-resistant enterococcal colonies that have been isolated from clinical or surveillance cultures. A standard number of bacteria is inoculated onto BHI agar containing 6 µg of vancomycin per ml. Following incubation, the appearance of growth indicates that the enterococcal isolate is likely to be resistant to vancomycin. MIC and species identification tests are subsequently required to determine if the isolate is a true vancomycin-resistant enterococcus (VRE) ( ).

Citation: Garcia L. 2010. Agar Screen Test To Detect Vancomycin Resistance in spp., p 57-60. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.6
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References

/content/book/10.1128/9781555817435.chap5.6
1. NCCLS. 2003. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 6th ed. Approved standard M7-A6. NCCLS, Wayne, Pa.
2. Swenson, J. M.,, J. F. Hindler,, and J. H. Jorgensen,. 2003. Special phenotypic methods for detecting antimicrobial resistance, p. 1178– 1195. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C.
3. Tenover, F. C.,, M. V. Lancaster,, B. C. Hill,, C. D. Steward,, S. A. Stocker,, G. A. Hancock,, C. M. O'Hara,, S. K. McAllister,, N. C. Clark,, and K. Hiramatsu. 1998. Characterization of staphylococci with reduced susceptibilities to vancomycin and other glycopeptides. J. Clin. Microbiol. 36: 1020– 1027.
4. Cetinkaya, Y.,, P. Falk,, and C. G. Mayhall. 2000 Vancomycin-resistant enterococci. Clin. Microbiol. Rev. 13: 686– 707.

Tables

Agar Screen Test To Detect Vancomycin Resistance in Enterococcus spp.
Citation: Garcia L. 2010. Agar Screen Test To Detect Vancomycin Resistance in spp., p 57-60. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.6
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Citation: Garcia L. 2010. Agar Screen Test To Detect Vancomycin Resistance in spp., p 57-60. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.6

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