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Chapter 7.4 : Liquid Media for Isolation

Editor: Lynne S. Garcia1
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Affiliations: 1: LSG & Associates, Santa Monica, California
Content Type: Reference
Publication Year: 2010

Category: Clinical Microbiology

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Liquid Media for Isolation, Page 1 of 2

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Abstract:

In the BACTEC 460TB radiometric system (Becton Dickinson Diagnostic Systems, Sparks, Md.), growth medium for culturing mycobacteria is supplemented with a substrate labeled with radioactive carbon (C). This substrate is utilized by mycobacteria, and during metabolism, carbon dioxide (C) is produced from the substrate. The C is detected quantitatively by counting the radioactivity with a BACTEC 460 instrument. The rate and amount of C produced are directly proportional to the rate and amount of growth occurring in the medium. This principle is applied for isolation of mycobacteria from clinical specimens, differentiation of the complex from other mycobacteria, and antimicrobial susceptibility testing.

Citation: Garcia L. 2010. Liquid Media for Isolation, p 301-325. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.4
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References

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Tables

Liquid Media for Isolation
Citation: Garcia L. 2010. Liquid Media for Isolation, p 301-325. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.4
/content/10.1128/9781555817435.chap7.4.tab1-p7.4.2.2
Untitled
10.1128/9781555817435/tab1-p7.4.2.2_thmb.gif
10.1128/9781555817435/tab1-p7.4.2.2.gif
Generic image for table
Untitled

Citation: Garcia L. 2010. Liquid Media for Isolation, p 301-325. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.4
/content/10.1128/9781555817435.chap7.4.tab7.4.3-1
Table 7.4.3–1

Expected QC results

10.1128/9781555817435/tab7.4.3-1_thmb.gif
10.1128/9781555817435/tab7.4.3-1.gif
Generic image for table
Table 7.4.3–1

Expected QC results

Citation: Garcia L. 2010. Liquid Media for Isolation, p 301-325. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.4
/content/10.1128/9781555817435.chap7.4.tab1-p7.4.4.2
Untitled
10.1128/9781555817435/tab1-p7.4.4.2_thmb.gif
10.1128/9781555817435/tab1-p7.4.4.2.gif
Generic image for table
Untitled

Citation: Garcia L. 2010. Liquid Media for Isolation, p 301-325. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.4

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