Chapter 7.5 : Septi-Chek AFB Biphasic Medium

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The BD BBL Septi-Chek AFB mycobacterial culture system (Becton Dickinson Microbiology Systems, Cockeysville, Md.) is a biphasic medium with a self-contained CO environment. It consists of Middlebrook 7H9 broth and a slide of Middlebrook 7H11, modified egg, and chocolate media. The system offers sensitivity comparable to those of conventional agar and broth methods ( ). The inclusion of CHOC in this system is advantageous because it detects contaminants and when incubated at 30°C, can be detected.

Citation: Garcia L. 2010. Septi-Chek AFB Biphasic Medium, p 326-328. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch7.5
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1. D'Amato, R. F.,, H. D. Isenberg,, L. Hochstein,, A. J. Mastellone,, and P. Alperstein. 1991. Evaluation of the Roche Septi-Chek AFB system for recovery of mycobacteria. J. Clin. Microbiol. 29: 2906 2908.
2. Isenberg, H. D.,, R. F. D'Amato,, L. Heifets,, P. R. Murray,, M. Scardamaglia,, M. C. Jacobs,, P. Alperstein,, and A. Niles. 1991. Collaborative feasibility study of biphasic system (Roche Septi-Chek AFB) for the rapid detection and isolation of mycobacteria. J. Clin. Microbiol. 29: 1719 1722.
3. Kent, P. T.,, and G. P. Kubica. 1985. Public Health Mycobacteriology. A Guide for the Level III Laboratory, p. 21 30. U.S. Department of Health and Human Services, Centers for Disease Control, Atlanta, Ga..
4. Kiehn, T. E.,, F. F. Edwards,, P. Brannon,, A. Y. Tsang,, M. Maio,, J. W. M. Gold,, E. Whimbey,, B. Wong,, J. K. McClatchy,, and D. Armstrong. 1985. Infections caused by Mycobacterium avium complex in immunocompromised patients: diagnosis by blood culture and fecal examination, antimicrobial susceptibility tests, and morphological and seroagglutination characteristics. J. Clin. Microbiol. 21: 168 173.

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