Chapter 5 : Surface Sampling

Abstract:

This chapter covers the following topics: surface sampling approaches, interpretation of results, preparation of surface sampling kits and tools, and an approach to handling suspicious-powder incidents. Two basic elements must be present in any plan to collect biological agents: the use of sterile equipment and the use of the aseptic technique. To ensure the reliability of the results, it is critical to ensure that the media, solutions, and sample containers are sterile and remain sterile until they contain sample material. The methods presented in the chapter include HEPA vacuum collection socks, microvacuuming, and bulk collection. As noted, the publications discussed here illustrate the need to evaluate each sampling device for its intended use and recovery of specific agents. Included in the chapter is a list of the materials needed to collect up to 50 samples from a variety of media for a variety of biological agents. Although surface sampling appears to be a simple task, the chapter conveys some of the complexities involved in obtaining samples and interpreting the information gathered from them. At the end of the day, a good sampling strategy for the various investigation phases, a consistent approach, an understanding of the limitations of the different sampling methods, and a thorough knowledge of the target organism are the most important factors in obtaining the best possible information from a surface sampling event.