Chapter 11 : DNA Scissors

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Genetic engineering is possible because of special enzymes that cut DNA. Restriction enzymes are proteins produced by bacteria to prevent or restrict invasion by foreign DNA. They act as DNA scissors, cutting the foreign DNA into pieces so that it cannot function. EcoRI makes one cut between the G and A in each of the DNA strands. The EcoRI cut sites are not directly across from each other on the DNA molecule. When scientists study a DNA molecule, one of the first things they do is to figure out where many restriction sites are. They then create a restriction map, showing the location of cleavage sites for many different enzymes. These maps are used like road maps to the DNA molecule. A restriction map of a plasmid is discussed in this chapter.

Citation: Kreuzer H, Massey A. 2008. DNA Scissors, p 188-189. In Molecular Biology and Biotechnology: A Guide for Students, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817480_ch11
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