Methods for General and Molecular Microbiology, Third Edition

Editors: C. A. Reddy1, T. J. Beveridge2, J. A. Breznak3, G. A. Marzluf4, T. M. Schmidt5, L. R. Snyder6
Affiliations: 1: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan; 2: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada; 3: Michigan State University, East Lansing, Michigan; 4: Department of Biochemistry, The Ohio State University, Columbus, Ohio; 5: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan; 6: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan
Content Type: Reference
Format: Electronic, Hardcover
Publication Year: 2007

Category: Microbial Genetics and Molecular Biology

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

After thirteen years, the editors of have prepared a new, state-of-the-art volume bringing this invaluable resource up to the present. This edition is much expanded with two entirely new sections on Community and Genomic Analysis and on Mycology (with emphasis on filamentous fungi) and 47 chapters compared to 31 in the previous edition.

This book will once again serve as a first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. Covering methods for microbes representing all three domains of life--Bacteria, , and --this manual should be on the bookshelf of every serious practitioner of microbiology in academia, industry, and government, and in clinical laboratories. It will serve as a rich resource on methods for seasoned professionals as well as for students at all levels.

Many of the new chapters cover important topics such as molecular genetics of ; molecular genetic manipulations using phages as tools; state-of-the-art technology used in studying complex, naturally occurring microbial communities; metabolism of natural polymers including cellulose, hemicellulose, and lignin; DNA microarray technology; physiology, metabolism, molecular genetic approaches to the study of filamentous fungi, and general and molecular approaches for studying microbial symbioses.

Each section details reliable methods that are widely applicable to basic studies. Additionally, chapters present background principles to facilitate understanding of the how and why of a given method, followed by a step-by-step description of the procedure. Common problems, precautions, and pitfalls of the methods are also presented. In many cases, commercial sources for equipment and materials are also given.

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Doody Enterprises

14 July 2013

At A Glance

This volume is a state-of-the-art revision of the previously published "Methods for General and Molecular Bacteriology". This new, expanded edition offers comprehensive coverage of methods and includes archaea and filamentous fungi, in addition to bacteria.


This new edition of a classic reference in microbiology is put together well. In over 1,000 pages, this book covers the multiple topics and techniques used to investigate microorganisms. With the previous edition of this book over 10 years old, this update was necessary to include the many new investigational tools developed since then.


The purpose of this book has remained constant over the years. The previous versions have become the standard source of information for microbiologists in all fields. This book is the "first source for traditional methods of microbiology as well as modern molecular biology." The new version includes new methods and theories as well as new areas of microbiology.


All scientists and students of microbiology will find this book a valuable resource. It is written at a level appropriate for professional scientists as well as training scientists. All of the chapters are well organized and are written by well established scientists familiar with both the science and technical details.


This book covers both the theory and practical considerations of the various techniques used to study microorganisms. The different sections cover microscopy, analysis of enzymatic reactions, microbial metabolism, and molecular analysis. The authors emphasize the need for multiple types of analyses in order to answer the multiple questions in microbiology. Each chapter contains the basic background and historical information needed to understand the new methods and each method is presented in detail that makes it easy to follow. There is also discussion of potential problems and how to avoid them. In some cases the authors have listed the equipment and reagents needed to successfully use the technique.


It is hard to improve on success, but this latest edition of a treasured classic has exceeded expectations. The original edition was my first "real" microbiology textbook and it will be good to have an up-to-date version. This latest edition can be relied upon to teach the new generation of scientists and will remain a treasure in my collection of classic references for many years.

Doody Enterprises

Reviewer: Rebecca Horvat, PhD, D(ABMM) (University of Kansas Medical Center)

Review Date: No Date

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