Introduction to Functional Genomics of the Mycobacterium tuberculosis Complex

Priscille Brodin; Caroline Demangel; Stewart T. Cole

doi:10.1128/9781555817657.ch9

Chapter 9 : Introduction to Functional Genomics of the Mycobacterium tuberculosis Complex

Authors: Priscille Brodin, Caroline Demangel, Stewart T. Cole¹

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Affiliations: 1: Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, 28 Rue du octeur Roux, 75724 Paris Cedex 15, France

Content Type: Monograph

Publication Year: 2005

Category: Bacterial Pathogenesis; Clinical Microbiology

Book DOI: 10.1128/9781555817657

Chapter DOI: 10.1128/9781555817657.ch9

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Abstract:

This chapter starts with a description of the three complete genome sequences of Mycobacterium tuberculosis H37Rv, M. tuberculosis CDC 1551, and Mycobacterium bovis AF2122/97 and highlights the genomic differences between members of the M. tuberculosis complex. Tremendous large-scale efforts have now led to the sequences of numerous genomes ranging from simple bacteria to complex organisms such as humans. M. tuberculosis has the metabolic potential to adapt to aerobic and microaerophilic as well as anaerobic environments. M. bovis, the bovine tubercle bacillus, has the broadest host tropism, and this includes humans. The greatest variation between M. bovis and M. tuberculosis has been identified among cell wall components and secreted proteins. The availability of the complete genome sequence of M. tuberculosis, combined with major progress in transcriptome technology, has led to the identification of a number of virulence genes. The classical proteome approach comprises protein separation by two-dimensional gel electrophoresis (2-DE) and spot identification by mass spectrometry or any biochemical or chemical cleavage techniques such as Edman degradation or enzymatic proteolysis. The goal of structural genomics is to apply high-throughput technologies to generate large amounts of recombinant proteins of M. tuberculosis for structure determination. Comparative genomics has provided insight into the coevolution of tubercle bacilli and their mammalian hosts, and one finding of some concern is the great diversity in the sequences of the PE and PPE proteins between strains.

Citation: Brodin P, Demangel C, Cole S. 2005. Introduction to Functional Genomics of the Mycobacterium tuberculosis Complex, p 141-154. In Cole S, Eisenach K, McMurray D, Jacobs, Jr. W (ed), Tuberculosis and the Tubercle Bacillus. ASM Press, Washington, DC. doi: 10.1128/9781555817657.ch9

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Introduction to Functional Genomics of the Mycobacterium tuberculosis Complex

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Figure 1

Distribution of the 4,043 genes in M. tuberculosis H37Rv according to their function.

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10.1128/9781555817657/fig9-1.gif

Figure 1

Distribution of the 4,043 genes in M. tuberculosis H37Rv according to their function.

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Figure 2.

Functional genomics scheme showing information flow from the genome to applications. The URLs of useful websites are indicated.

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10.1128/9781555817657/fig9-2.gif

Figure 2.

Functional genomics scheme showing information flow from the genome to applications. The URLs of useful websites are indicated.

References

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