Commercial Methods in Clinical Veterinary Microbiology

Thomas J. Inzana; David S. Lindsay; Xiang-Jin Meng; Karen W. Post

doi:10.1128/9781555817961.ch14

Chapter 14 : Commercial Methods in Clinical Veterinary Microbiology

Authors: Thomas J. Inzana, David S. Lindsay, Xiang-Jin Meng, Karen W. Post

Content Type: Reference

Publication Year: 2002

Category: Clinical Microbiology

Book DOI: 10.1128/9781555817961

Chapter DOI: 10.1128/9781555817961.ch14

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Abstract:

This chapter describes, and in some cases reports evaluations of, those commercial identification systems that are used by veterinary diagnostic laboratories. Most modern viral diagnostic tests are rapid assays aimed at detection of antiviral antibodies (enzyme-linked immunosorbent assay [ELISA], Western immunoblot analysis, immunofluorescence, serum virus neutralization [SVN], hemagglutination inhibition [HI], agar gel immunodiffusion [AGID], and complement fixation [CF]), detection of viral antigens (immunohistochemistry [IHC], antigen-capture ELISA, and frozen tissue immunofluorescence), or detection (or amplification) of viral nucleic acids (PCR and its related techniques, in situ hybridization [ISH], Southern blot hybridization, and dot blot hybridization). In addition, modern molecular tests and procedures (such as PCR-restriction fragment length polymorphism [PCR-RFLP] analysis, sequencing, and sequence analysis) are being used more commonly for further genetic characterization of viruses and for differential diagnosis. With a known viral antigen, latex agglutination test (LAT) can also be used to detect antiviral antibody, such as that for swine pseudorabies virus. LAT kits are commercially available for a few veterinary viruses. The chapter also focuses on reviews of studies that have evaluated the applicability of bacterial identification systems for identification of bacteria isolated from animal specimens. The identification of dermatophytes from animal specimens is emphasized in the chapter because dermatophytes are causes of zoonotic infections as well as common infections in animals. Identification of mites, lice, ticks, fleas, and fly larvae infecting animals is a common occurrence for veterinary parasitologists.

Citation: Inzana T, Lindsay D, Meng X, Post K. 2002. Commercial Methods in Clinical Veterinary Microbiology, p 336-359. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch14

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Porcine reproductive and respiratory syndrome virus: 0.50807995
Equine infectious anemia virus: 0.41549802

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Commercial Methods in Clinical Veterinary Microbiology

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FIGURE 1

IHC of porcine reproductive and respiratory syndrome virus (PRRSV) antigen in the alveolar macrophages of a lung from a pig infected with PRRSV. Alveolar macrophages show dark brown staining of PRRSV antigen. Shown is the result of avidin-biotin-peroxidase complex staining and hematoxylin counterstain. Courtesy of Patrick G. Halbur, Iowa State University.

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FIGURE 1

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FIGURE 2

ISH detection of porcine reproductive and respiratory syndrome virus (PRRSV) nucleic acid in the alveolar macrophages of a lung from a pig infected with PRRSV. PRRSV nucleic acid is detected in the cytoplasm of macrophages. Shown is the result of ISH with a digoxigenin-labeled PRRSV riboprobe detected by antidigoxigenin-alkaline phosphatase complex, and nuclear fast red counterstain. Courtesy of Patrick G. Halbur, Iowa State University.

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FIGURE 2

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FIGURE 3

Detection and differentiation of porcine circovirus type 1 and 2 infections by a PCR-RFLP assay. (A) PCR amplification of a 243-bp fragment. Lane 1, negative control sample; lanes 2 to 13, samples containing porcine circovirus (both type 1 and 2). (B) RFLP analysis of the PCR products. Lanes L, 50-bp DNA ladder; lane 1, a sample of liver tissue from a control specific-pathogen-free pig; lanes 2 to 11, clinical samples from 10 cases of porcine circovirus type 2; lane 12, PK15 cells containing porcine circovirus type 1; lane 13, a sample containing porcine circoviruses (both type 1 and 2). The expected PCR fragment (A) and three RFLP fragments, 243, 168, and 75 bp, respectively (B) are indicated with arrows. Modified from Fenaux et al. (25).

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FIGURE 3

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FIGURE 4

HESKA Solo Step canine heartworm antigen diagnostic test. The test is a flow technology ELISA-based test. A positive test results in two bands on the membrane (control band and test band), whereas a negative test will result in only one band (control band).

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FIGURE 4

References

/content/book/10.1128/9781555817961.chap14

1. Adney, W. S.,, and R. L. Jones. 1985. Evaluation of the RapID-ANA sytem for the identification of anaerobic bacteria of veterinary origin. J. Clin. Microbiol. 22: 980– 983.

2. Ali, A.,, and D. L. Reynolds. 1999. A reverse transcription-polymerase chain reaction assay for the detection of avian pneumovirus (Colorado strain). Avian Dis. 43: 600– 603.

3.Anonymous. 1984. Pictorial Keys to Arthropods of Reptiles, Birds and Mammals of Public Health Significance. Public Health Service, Bureau of Disease Prevention and Environmental Control, National Communicable Disease Center, Atlanta, Ga..

4. Bannister, M. F.,, C. E. Benson,, and C. R. Sweeney. 1985. Rapid species identification of group C streptococci from horses. J. Clin. Microbiol. 21: 524– 526.

5. Belgrader, P.,, W. Benett,, D. Hadley,, J. Richards,, P. Stratton,, R. Mariella,, and F. Milanovich. 1999. PCR detection of bacteria in seven minutes. Science 284: 449– 450.

6. Benfield, D. A.,, I. J. Stotz,, E. A. Nelson,, and K. S. Groon. 1984. Comparison of a commercial enzyme-linked immunosorbent assay with electron microscopy, fluorescent antibody, and virus isolation for the detection of bovine and porcine rotavirus. Am. J. Vet. Res. 45: 1998– 2002.

7. Biberstein, E. L.,, S. S. Jang,, and D. W. Hirsch. 1984. Species distribution of coagulase-positive staphylococci in animals. J. Clin. Microbiol. 19: 610– 615.

8. Bodeus, M.,, C. Cambiaso,, M. Surleraux,, and G. Burtonboy. 1988. A latex agglutination test for the detection of canine parvovirus and corresponding antibodies. J. Virol. Methods 19: 1– 12.

9. Borchers, K.,, U. Wolfinger,, B. Lawrenz,, A. Schellenbach,, and H. Ludwig. 1997. Equine herpesvirus 4 DNA in trigerminal ganglia of naturally infected horses detected by direct in situ PCR. J. Gen. Virol. 78: 1109– 1114.

10. Bougrine, S. I.,, O. F. Fihri,, and M. M. Fehri. 1998. Western immunoblotting as a method for the detection of African horse sickness virus protein-specific antibodies: differentiation between infected and vaccinated horses. Arch. Virol. Suppl. 14: 329– 336.

11. Bowman, D. D. 1999. Georgis’ Parasitology for Veterinarians. W. B. Saunders Company, Philadelphia, Pa..

12. Bridger, J. C. 1980. Detection by electron microscopy of caliciviruses, astroviruses and rotavirus-like particles in the feces of piglets with diarrhea. Vet. Rec. 107: 532– 533.

13. Bryan, L. A.,, J. R. Campbell,, A. A. Gajadhar. 1999. Effects of temperature on the survival of Tritrichomonas foetus in transport, Diamond’s and InPouch TF media. Vet. Rec. 144: 227– 232.

14. Burki, F.,, W. Rossmanith,, and E. Rossmanith. 1992. Equine lentivirus, comparative studies on four serological tests for the diagnosis of equine infectious anemia. Vet. Microbiol. 33: 353– 360.

15. Carter, G. R.,, and J. R. Cole. 1990. Diagnostic Procedures in Veterinary Bacteriology and Mycology, 5th ed. Academic Press, San Diego, Calif..

16. Chua, K. B.,, W. J. Bellini,, P. A. Rota,, B. H. Harcourt,, A. Tamin,, S. K. Lam,, T. G. Ksiazek,, P. E. Rollin,, S. R. Zaki,, W. Shieh,, C. S. Goldsmith,, D. J. Gubler,, J. T. Roehrig,, B. Eaton,, A. R. Gould,, J. Olson,, H. Field,, P. Daniels,, A. E. Ling,, C. J. Peters,, L. J. Anderson,, and B. W. Mahy. 2000. Nipah virus: a recently emergent deadly paramyxovirus. Science 288: 1432– 1435.

17. Collins, J. K.,, C. A. Riegel,, J. D. Olson,, and A. Fountain. 1987. Shedding of enteric coronavirus in adult cattle. Am. J. Vet. Res. 48: 361– 365.

18. Collins, M. T. 1996. Diagnosis of paratubersulosis. Vet. Clin. N. Am. Food Anim. Pract. 12: 357– 371.

19. Collins, M. T.,, K. B. Kenefick,, D. C. Sockett,, R. S. Lambrecht,, J. McDonald,, and J. B. Jorgensen. 1990. Enhanced radiometric detection of Mycobacterium paratuberculosis using filter concentrated fecal specimens. J. Clin. Microbiol. 28: 2514– 2519.

20. Collins, M. T.,, and E. C. Swanson. 1981. Use of the API 20E system to identify non-enterobacteriaceae from veterinary medical sources. Am. J. Vet. Res. 42: 1269– 1273.

21. Collins, M. T.,, N. Weaver,, and R. P. Ellis. 1981. Identification of Pasteurella multocida and Pasteurella haemolytica by API 20E, Minitek and Oxi-Ferm systems. J. Clin. Microbiol. 23: 433– 437.

22. Cox, H. U.,, J. D. Hoskins,, S. S. Newman,, G. H. Turnwald,, C. S. Foil,, A. F. Roy,, and M. T. Kearney. 1985. Distribution of staphylococcal species on clinically healthy cats. Am. J. Vet. Res. 46: 1824– 1828.

23. Dar, A. M.,, S. Kapil,, and S. M. Goyal. 1998. Comparison of immunohistochemistry, electron microscopy, and direct fluorescent antibody test for the detection of bovine coronavirus. J. Vet. Diagn. Investig. 10: 152– 157.

24. Elder, J.,, and C. Brown. 1999. Review of techniques for the diagnosis of Chlamydia psittaci infection in psittacine birds. J. Vet. Diagn. Investig. 11: 539– 541.

25. Fenaux, M.,, P. G. Halbur,, M. Gill,, T. E. Toth,, and X.-J. Meng. 2000. Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J. Clin. Microbiol. 38: 2494– 2503.

26. Finlaison, D. S. 1995. Fecal viruses of dogs—an electron microscope study. Vet. Microbiol. 46: 295– 305.

27. Gamble, D. A.,, A. Lobbiani,, M. Gramegna,, L. E. Moore,, and G. Colucci. 1997. Development of a nested PCR assay for detection of feline infectious peritonitis virus in clinical specimens. J. Clin. Microbiol. 35: 673– 675.

28. Gaudi, S.,, W. Ponti,, A. Agresti,, R. Meneveri,, M. Malcovati,, L. Bonizzi,, G. Poli,, A. Amato,, and E. Ginelli. 1990. Detection of bovine leukaemia virus (BLV) infection by DNA probe technology. Mol. Cell. Probes 4: 163– 174.

29. Gelmetti, D.,, V. Grieco,, C. Rossi,, L. Capucci,, and A. Lavazza. 1998. Detection of rabbit haemorrhagic disease virus (RHDV) by in situ hybridization with a digoxigenin labeled RNA probe. J. Virol. Methods 72: 219– 226.

30. Gilbert, S. A.,, K. M. Burton,, S. E. Prins,, and D. Deregt. 1999. Typing of bovine viral diarrhea viruses directly from blood of persistently infected cattle by multiplex PCR. J. Clin. Microbiol. 37: 2020-– 2023.

31. Gillette, K. G. 1983. Enzyme-linked immunosorbent assay for serum antibody to bovine respiratory syncytial virus: comparison with complement-fixation and neutralization tests. Am. J. Vet. Res. 44: 2251– 2255.

32. Gottschalk, M.,, R. Higgins,, M. Jacques,, M. Beaudoin,, and J. Henrichsen. 1991. Characterization of six new capsular types (23-28) of Streptococcus suis. J. Vet. Diagn. Investig. 29: 2590– 2594.

33. Gottschalk, M.,, R. Higgins,, M. Jacques,, M. Beaudoin,, and J. Henrichsen. 1991. Isolation and characterization of Streptococcus suis capsular types 9-22. J. Vet. Diagn. Investig. 3: 60– 65.

34. Guo, W. Z.,, D. T. Shen,, J. F. Evermann,, and J. R. Gorham. 1988. Comparison of an enzyme-linked immunosorbent assay and a complement-fixation test for the detection of IgG to bovine herpesvirus type 4 (bovine cytomegalovirus). Am. J. Vet. Res. 49: 667– 670.

35. Gut, M.,, C. M. Leutenegger,, J. B. Huder,, N. C. Pedersen,, and H. Lutz. 1999. One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses. J. Virol. Methods 77: 37– 46.

36. Halbur, P. G.,, J. J. Andrews,, E. L. Huffman,, P. S. Paul,, X.-J. Meng,, and Y. Niyo. 1994. Development of a streptavidin-biotin immunoperoxidase procedure for the detection of porcine reproductive and respiratory syndrome virus antigen in porcine lung. J. Vet. Diagn. Investig. 6: 254– 257.

37. Hammami, S.,, A. E. Castro,, and B. I. Osburn. 1990. Comparison of polyacrylamide gel electrophoresis, an enzyme-linked-immunosorbent assay, and an agglutination test for the direct identification of bovine rotavirus from feces and coelectrophoresis of viral RNAs. J. Vet. Diagn. Investig. 2: 184– 190.

38. Hassan, M. K.,, Y. M. Saif,, and S. Shawky. 1996. Comparison between antigen-capture ELISA and conventional methods used for titration of infectious bursal disease virus. Avian Dis. 40: 562– 566.

39. Hawkes, R. A.,, P. D. Kirkland,, D. A. Sanders,, F. Zhang,, Z. Li,, R. J. Davis,, and N. Zhang. 2000. Laboratory and field studies of an antigen capture ELISA for bluetongue virus. J. Virol. Methods 85: 137– 149.

40. Haynes, J. S.,, P. G. Halbur,, T. Sirinarumitr,, P. S. Paul,, X.-J. Meng,, and E. L. Huffman. 1997. Temporal and morphologic characterization of the distribution of porcine reproductive and respiratory syndrome virus (PRRSV) by in situ hybridization in pigs infected with isolates of PRRSV that differ in virulence. Vet. Pathol. 34: 39– 43.

41. Heckert, R. A.,, L. J. Saif,, and G. W. Myers. 1989. Development of protein A-gold immunoelectron microscopy for detection of bovine coronavirus in calves: comparison with ELISA and direct immunofluorescence of nasal epithelial cells. Vet. Microbiol. 19: 217– 231.

42. Henderson, K. S.,, and D. J. Jackwood. 1990. Comparison of the dot blot hybridization assay with antigen detection assays for the diagnosis of infectious bursal disease virus infections. Avian Dis. 34: 744– 748.

43. Herrmann, J. E., 1995. Immunoassays for the diagnosis of infectious diseases, p. 110– 122. In P. R. Murray,, E. J. Baron,, M. A. Pfaller,, F. C. Tenover,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 6th ed. American Society for Microbiology, Washington, D.C..

44. Hierholzer, J. C., 1991. Rapid diagnosis of viral infection, p. 556– 590. In A. Vaheri, and A. Balows (ed.), Rapid Methods and Automation in Microbiology and Immunology. Springer-Verlag, New York, N.Y..

45. Hohdatsu, T.,, K. Motokawa,, M. Usami,, M. Amioka,, S. Okada,, and H. Koyama. 1998. Genetic subtyping and epidemiological study of feline immunodeficiency virus by nested polymerase chain reaction-restriction fragment length polymorphism analysis of the gag gene. J. Virol. Methods 70: 107– 111.

46. Horiuchi, M.,, K. Yuri,, T. Soma,, H. Katae,, H. Nagasawa,, and M. Shinagawa. 1996. Differentiation of vaccine virus from field isolates of feline panleukopenia virus by polymerase chain reaction and restriction fragment length polymorphism analysis. Vet. Microbiol. 53: 283– 293.

47. Hughes, J. H. 1993. Physical and chemical methods for enhancing rapid detection of viruses and other agents. Clin. Microbiol. Rev. 6: 150– 175.

48. Inzana, T. J.,, and B. Iritani. 1989. Rapid detection of group C streptococci from animals by latex agglutination. J. Clin. Microbiol. 27: 309– 312.

49. Ishikawa, K.,, H. Sekiguchi,, T. Ogino,, and S. Suzuki. 1997. Direct and rapid detection of porcine epidemic diarrhea virus by RT-PCR. J. Virol. Methods 69: 191– 195.

50. Ivens, V. R.,, D. L. Mark,, and N. D. Levine. 1978. Principal Parasites of Domestic Animals in the United States. Biological and Diagnostic Information. University of Illinois Press, Urbana-Champaign..

51. Jackson, M. L.,, D. M. Haines,, S. M. Meric,, and V. Misra. 1993. Feline leukemia virus detection by immunohistochemistry and polymerase chain reaction in formalin-fixed, paraffin-embedded tumor tissue from cats with lymphosarcoma. Can. J. Vet. Res. 57: 269– 276.

52. James, K. 1990. Immunoserology of infectious diseases. Clin. Microbiol. Rev. 3: 132– 152.

53. Jasper, D. E.,, F. Infante,, and J. D. Dellinger. 1985. Efficacy of the API Staph-Ident system for identification of Staphylococcus species from milk. Am. J. Vet. Res. 46: 1263– 1267.

54. Jayarao, B.,, S. P. Oliver,, K. R. Matthews,, and S. H. King. 1991. Comparative evaluation of Vitek gram-positive identification system and API Rapid Strep system for identification of Streptococcus species of bovine origin. Vet. Microbiol. 26: 301– 308.

55. Johnson, F. B. 1990. Transport of viral specimens. Clin. Microbiol. Rev. 3: 120– 131.

56. Johnson, M.,, F. Rommel,, and J. Mone. 1998. Development of a syncytia inhibition assay for the detection of antibodies to bovine leukemia virus in naturally infected cattle; comparison with Western blot and agar gel immunodiffusion. J. Virol. Methods 70: 177– 182.

57. Jones, R. L.,, W. S. Adney,, M. A. Davis,, H. Vonbyren,, and G. Thompson. 1987. Evaluation of Quantum II microbiology system for identification of gram-negative bacteria of veterinary origin. J. Clin. Microbiol. 25: 2071– 2074.

58. Kessler, C.,, H. J. Holtke,, R. Seibl,, J. Burg,, and K. Muhlegger. 1990. Non-radioactive labeling and detection of nucleic acids. I. A novel DNA labeling and detection system based on digoxigenin: anti-digoxigenin ELISA principle (digoxigenin system). Biol. Chem. Hoppe-Seyler 371: 917– 927.

59. Kies, L. M.,, M. C. DeBey,, and R. F. Ross. 1991. Identification of porcine mycoplasmas using commercial enzyme assay systems. J. Vet. Diagn. Investig. 3: 348– 350.

60. Kim, H. S.,, H. S. Joo,, W. T. Christianson,, and R. B. Morrison. 1991. Evaluation of serologic methods for the detection of antibodies to encephalomyocarditis virus in swine fetal thoracic fluids. J. Vet. Diagn. Investig. 3: 283– 286.

61. Kim, O.,, and C. Chae. 2000. In situ hybridization for the detection and localization of porcine epidemic diarrhea virus in the intestinal tissues from naturally infected piglets. Vet. Pathol. 37: 62– 67.

62. Kloos, W. E.,, and J. F. Wolfshohl. 1982. Identification of Staphylococcus species with the API STAPH-IDENT system. J. Clin. Microbiol. 16: 509– 516.

63. Krell, P. J.,, T. Salas,, and R. P. Johnson. 1988. Mapping of porcine parvovirus DNA and development of a diagnostic DNA probe. Vet. Microbiol. 17: 29– 43.

64. Langer, P. R.,, A. A. Waldrop,, and D. C. Ward. 1981. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc. Natl. Acad. Sci. USA 78: 6633– 6637.

65. Langlois, B. E.,, R. J. Harmon,, and K. Akers. 1983. Identification of Staphylococcus species of bovine origin with the API Staph-Ident system. J. Clin. Microbiol. 18: 1212– 1219.

66. Langlois, B. E.,, R. J. Harmon,, and K. Akers. 1984. Identification of Staphylococcus species of bovine origin with the DMS Staph-Trac system. J. Clin. Microbiol. 20: 227– 230.

67. Larochelle, R.,, H. Mardassi,, S. Dea,, and R. Magar. 1996. Detection of porcine reproductive and respiratory syndrome virus in cell cultures and formalin-fixed tissues by in situ hybridization using a digoxigeninlabeled probe. J. Vet. Diagn. Investig. 8: 3– 10.

68. Larochelle, R.,, M. Antaya,, M. Morin,, and R. Magar. 1999. Typing of porcine circovirus in clinical specimens by multiplex PCR. J. Virol. Methods 80: 69– 75.

69. Latimer, K. S.,, F. D. Niagro,, O. C. Williams,, A. Ramis,, M. A. Goodwin,, B. W. Ritchie,, and R. P. Campagnoli. 1997. Diagnosis of avian adenovirus infections using DNA in situ hybridization. Avian Dis. 41: 773– 782.

70. Lennette, D. A., 1995. Collection and preparation of specimens for virological examination, p. 868– 875. In P. R. Murray,, E. J. Baron,, M. A. Pfaller,, F. C. Tenover,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 6th ed. American Society for Microbiology, Washington, D.C..

71. Lennette, E. H.,, P. Halonen,, and F. A. Murphy. 1988. Laboratory Diagnosis of Infectious Diseases. Principles and Practices, vol. 2. Springer-Verlag, New York, N.Y..

72. Leutenegger, C. M.,, D. Klein,, R. Hofmann-Lehmann,, C. Mislin,, U. Hummel,, J. Boni,, F. Boretti,, W. H. Guenzburg,, and H. Lutz. 1999. Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan fluorogenic real-time detection system. J. Virol. Methods 78: 105– 116.

73. Levy, D.,, L. Deshayes,, B. Guillemain,, and A. L. Parodi. 1977. Bovine leukemia virus specific antibodies among French cattle. I. Comparison of complement fixation and hematological tests. Int. J. Cancer 19: 822– 827.

74. Lin, T. M.,, G. Y. Shi,, S. J. Jiang,, C. F. Tsai,, B. J. Hwang,, C. T. Hsieh,, and H. L. Wu. 1999. Persistent infection of bovine herpesvirus type 4 in bovine endothelial cell cultures. Vet. Microbiol. 70: 41– 53.

75. Liu, H. J.,, M. H. Liao,, C. D. Chang,, J. H. Chen,, M. Y. Lin,, and M. C. Tung. 1999. Comparison of two molecular techniques for the detection of avian reoviruses in formalin-fixed, paraffin-embedded chicken tissues. J. Virol. Methods 80: 197– 201.

76. Liu, X.,, J. J. Giambrone,, and E. J. Hoerr. 2000. In situ hybridization, immunohistochemistry, and in situ reverse transcription-polymerase chain reaction for detection of infectious bursal disease virus. Avian Dis. 44: 161– 169.

77. Lun, Z.,, S. Parker,, and A. A. Gajadhar. 2000. Comparison of growth rates of Tritrichomonas foetus isolates from various geographic regions using three different culture media. Vet. Parasitol. 89: 199– 208.

78. Maddox, R. L.,, and G. Koehne. 1982. Identification of Staphylococcus hyicus with the API Staph strip. J. Clin. Microbiol. 15: 984– 986.

79. Magar, R.,, and R. Larochelle. 1992. Immunohistochemical detection of porcine rotavirus using immunogold silver staining (IGSS). J. Vet. Diagn. Investig. 4: 3– 7.

80. Magar, R.,, R. Larochelle,, Y. Robinson,, and C. Dubuc. 1993. Immunohistochemical detection of porcine reproductive and respiratory syndrome virus using colloidal gold. Can. J. Vet. Res. 57: 300– 304.

81. Mandell, G. L.,, J. E. Bennett,, and R. Dolin. 1995. Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases, 4th ed., vol 1. Churchill Livingstone, New York, N.Y..

82. Manni, V.,, F. Roperto,, G. Di Guardo,, D. Galati,, R. U. Condoleo,, and A. Venuti. 1998. Presence of papillomavirus-like DNA sequences in cutaneous fibropapillomas of the goat udder. Vet. Microbiol. 61: 1– 6.

83. Marshall, J. A.,, M. L. Kennett,, S. M. Rodger,, M. J. Studdert,, W. L. Thompson,, and I. D. Gust. 1987. Virus and virus-like particles in the feces of cats with and without diarrhea. Aust. Vet. J. 64: 100– 105.

84. Martinez, M. L.,, and R. C. Weiss. 1993. Detection of feline infectious peritonitis virus infection in cell cultures and peripheral blood mononuclear leukocytes of experimentally infected cats using a biotinylated cDNA probe. Vet. Microbiol. 34: 259– 271.

85. Martini, M.,, G. Capelli,, G. Poglayen,, F. Bertotti,, and C. Turilli. 1996. The validity of some haematological and ELISA methods for the diagnosis of canine heartworm disease. Vet. Res. Commun. 20: 331– 339.

86. Matthews, K. R.,, S. P. Oliver,, and S. H. King. 1990. Comparison of Vitek gram-positive identification system with API Staph-Trac for species identification of staphylococci of bovine origin. J. Clin. Microbiol. 28: 1649– 1651.

87. McGoldrick, A.,, J. P. Lowings,, G. Ibata,, J. J. Sands,, S. Belak,, and D. J. Paton. 1998. A novel approach to the detection of classical swine fever virus by RT-PCR with a fluorogenic probe (TaqMan). J. Virol. Methods 72: 125– 135.

88. McIntosh, K., 1996. Diagnostic virology, p. 401– 430. In B. N. Fields,, D. M. Knipe,, P. M. Howley,, R. M. Chanock,, J. L. Melnick,, T. P. Monath,, B. Roizman,, and A. E. Straus (ed.), Fields Virology, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, Pa..

89. McNeilly, F.,, S. Kennedy,, D. Moffett,, B. M. Meehan,, J. C. Foster,, E. G. Clarke,, J. A. Ellis,, D. M. Haines,, B. M. Adair,, and G. M. Allan. 1999. A comparison of in situ hybridization and immunohistochemistry for the detection of a new porcine circovirus in formalin-fixed tissues from pigs with postweaning multisystemic wasting syndrome (PMWS). J. Virol. Methods 80: 123– 128.

90. Meng, X.-J.,, P. S. Paul,, E. M. Vaughn,, and J. J. Zimmerman. 1993. Development of a radiolabeled nucleic acid probe for the detection of encephalomyocarditis virus of swine. J. Vet. Diagn. Investig. 5: 254– 258.

91. Meng, X.-J.,, P. S. Paul,, P. G. Halbur,, and M. A. Lum. 1996. Characterization of a high-virulence US isolate of porcine reproductive and respiratory syndrome virus in a continuous cell line, ATCC CRL11171. J. Vet. Diagn. Investig. 8: 374– 381.

92. Moody, A.,, S. Sellers,, and N. Bumstead. 2000. Measuring infectious bursal disease virus RNA in blood by multiplex real-time quantitative RT-PCR. J. Virol. Methods 85: 55– 64.

93. Morrison, J. R. A.,, and G. S. Tillotson. 1988. Identification of Actinomyces (Corynebacterium) pyogenes with the API 20 Strep system. J. Clin. Microbiol. 26: 1865– 1866.

94. Mulder, W. A.,, F. van Poelwijk,, R. J. Moormann,, B. Reus,, G. L. Kok,, J. M. Pol,, and A. Dekker. 1997. Detection of early infection of swine vesicular disease virus in porcine cells and skin sections. A comparison of immunohistochemistry and in-situ hybridization. J. Virol. Methods 68: 169– 175.

95. Murphy, F. A.,, E. P. J. Gibbs,, M. C. Horzinek,, and M. J. Studdert. 1999. Veterinary Virology, 3rd ed. Academic Press, New York, N.Y..

96. Nussbaum, D. J.,, J. R. Salord,, and D. D. Rimmele. 1999. Evaluation of quantitative latex agglutination for detection of Cryptosporidium parvum, E. coli K99, and rotavirus in calf feces. J. Vet. Diagn. Investig. 11: 314– 318.

97. Oliver, R. E.,, A. I. Donaldson,, C. F. Gibson,, P. L. Roeder,, P. M. Le Blanc Smith,, and C. Hamblin. 1988. Detection of foot-and-mouth disease antigen in bovine epithelial samples: comparison of sites of sample collection by an enzyme linked immunosorbent assay (ELISA) and complement fixation test. Res. Vet. Sci. 44: 315– 319.

98. Palmer, E. L.,, and M. L. Martin. 1988. Electron Microscopy in Viral Diagnosis. CRC Press, Boca Raton, Fla..

99. Papp, J. R.,, and C. A. Muckle. 1991. Evaluation of the Sceptor system for identification of bacteria of veterinary origin. J. Clin. Microbiol. 29: 10– 15.

100. Parwani, A. V.,, B. I. Rosen,, J. Flores,, M. A. McCrae,, M. Gorziglia,, and L. J. Saif. 1992. Detection and differentiation of bovine group A rotavirus serotypes using polymerase chain reaction-generated probes to the VP7 gene. J. Vet. Diagn. Investig. 4: 148– 158.

101. Patten, V. H.,, S. J. Shin,, J. Cole,, C. W. Watson,, and W. H. Fales. 1995. Evaluation of a commercial automated system and software for the identification of veterinary bacterial isolates. J. Vet. Diagn. Investig. 7: 506– 508.

102. Patterson, J. S.,, R. K. Maes,, T. P. Mullaney,, and C. L. Benson. 1996. Immunohistochemical diagnosis of eastern equine encephalomyelitis. J. Vet. Diagn. Investig. 8: 156– 160.

103. Peele, D.,, J. Bradford,, W. Pryor,, and S. Vore. 1997. Comparison of identifications of human and animal source gram-negative bacteria by API 20E and Crystal E/NF systems. J. Clin. Microbiol. 35: 213– 216.

104. Podzorski, R. P.,, and D. H. Persing,. 1995. Molecular detection and identification of microorganisms, p. 130– 157. In P. R. Murray,, E. J. Baron,, M. A. Pfaller,, F. C. Tenover,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 6th ed. American Society for Microbiology, Washington, D.C..

105. Post, K. W.,, S. C. Murphy,, J. B. Boyette,, and P. M. Resseguie. 1999. Evaluation of a commercial system for the identification of Ornithobacterium rhinotracheale. J. Vet. Diagn. Investig. 11: 97– 99.

106. Poultrel, B.,, and H. Z. Ryniewicz. 1984. Evaluation of the API 20 Strep system for species identification of streptococci isolated from bovine mastitis. J. Clin. Microbiol. 19: 213– 214.

107. Pratelli, A.,, M. Tempesta,, G. Greco,, V. Martella,, and C. Buonavoglia. 1999. Development of a nested PCR assay for the detection of canine coronavirus. J. Virol. Methods 80: 11– 15.

108. Quinn, P. J.,, J. C. Donnelly,, M. E. Carter,, B. K. J. Markey,, P. R. Torgerson,, and R. M. S. Breathnach. 1997. Microbial and Parasitic Diseases of the Dog and Cat. W. B. Saunders, London, United Kingdom.

109. Rather, P. N.,, A. P. Davis,, and B. J. Wilkinson. 1986. Slime production by bovine milk Staphylococcus aureus and identification of coagulase-negative staphylococcal isolates. J. Clin. Microbiol. 23: 858– 862.

110. Renshaw, R. W.,, R. Ray,, and E. J. Dubovi. 2000. Comparison of virus isolation and reverse transcription polymerase chain reaction assay for detection of bovine viral diarrhea virus in bulk milk tank samples. J. Vet. Diagn. Investig. 12: 184– 186.

111. Reubel, G. H.,, B. S. Crabb,, and M. J. Studdert. 1995. Diagnosis of equine gammaherpesvirus 2 and 5 infections by polymerase chain reaction. Arch. Virol. 140: 1049– 1060.

112. Rimstad, E.,, N. East,, E. DeRock,, J. Higgins,, and N. C. Pedersen. 1994. Detection of antibodies to caprine arthritis-encephalitis virus using recombinant gag proteins. Arch. Virol. 134: 345– 356.

113. Rocha, M. A.,, E. F. Barbosa,, S. E. Guimaraes,, E. D. Neto,, and A. M. Gouveia. 1998. A high sensitivity-nested PCR assay for BHV-1 detection in semen of naturally infected bulls. Vet. Microbiol. 63: 1– 11.

114. Salmon, S. A.,, J. L. Watts,, R. D. Walker,, and R. J. Yancey. 1995. Evaluation of a commercial system for the identification of gram-negative, nonfermenting bacteria of veterinary importance. J. Vet. Diagn. Investig. 7: 161– 164.

115. Salmon, S. A.,, J. L. Watts,, and R. J. Yancey. 1993. Evaluation of the RapID NH system for identification of Haemophilus somnus, Pasteurella multocida, Pasteurella haemolytica and Actinobacillus pleuropneumoniae isolated from cattle and pigs with respiratory disease. J. Clin. Microbiol. 31: 1362– 1363.

116. Sander, J.,, R. Williams,, R. Novak,, and W. Ragland. 1997. In situ hybridization on blood smears for diagnosis of chicken anemia virus in broiler breeder flocks. Avian Dis. 41: 988– 992.

117. Sanderson, T. P.,, and A. A. Andersen. 1992. Evaluation of a commercial solid-phase enzyme immunoassay for the detection of ovine Chlamydia psittaci. J. Vet. Diagn. 4: 192– 193.

118. Sanderson, T. P.,, and A. A. Andersen. 1989. Evaluation of an enzyme immunoassay for detection of Chlamydia psittaci in vaginal secretions, placentas, and fetal tissues from aborting ewes. J. Vet. Diagn. 1: 309– 315.

119. Sandvik, T.,, and J. Krogsrud. 1995. Evaluation of an antigen-capture ELISA for detection of bovine viral diarrhea virus in cattle blood samples. J. Vet. Diagn. Investig. 7: 65– 71.

120. Sanekata, T.,, E. Kishimoto,, K. Sato,, H. Honma,, K. Otsuki,, and M. Tsubokura. 1991. Detection of porcine rotavirus in stools by a latex agglutination test. Vet. Microbiol. 27: 245– 251.

121. Scheld, W. M.,, D. Armstrong,, and J. M. Hughes (ed.). 1997. Emerging Infections 1. ASM Press, Washington, D.C..

122. Scheld, W. M.,, W. A. Craig,, and J. M. Hughes (ed.). 1998 Emerging Infections 2. ASM Press, Washington, D.C..

123. Scheld, W. M.,, W. A. Craig,, and J. M. Hughes (ed.). 1999. Emerging Infections 3. ASM Press, Washington, D.C..

124. Schoenthaler, S. L.,, and S. Kapil. 1999. Development and applications of a bovine coronavirus antigen detection enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol. 6: 130– 132.

125. Schulze, C.,, and W. Baumgartner. 1998. Nested polymerase chain reaction and in situ hybridization for diagnosis of canine herpesvirus infection in puppies. Vet. Pathol. 35: 209– 217.

126. Sentsui, H.,, T. Nishimori,, I. Nagai,, and N. Nishioka. 1996. Detection of sheep-associated malignant catarrhal fever virus antibodies by complement fixation tests. J. Vet. Med. Sci. 58: 1– 5.

127. Shockley, L. J.,, P. A. Kapke,, W. Lapps,, D. A. Brian,, L. N. Potgieter,, and R. Woods. 1987. Diagnosis of porcine and bovine enteric coronavirus infections using cloned cDNA probes. J. Clin. Microbiol. 25: 1591– 1596.

128. Simard, C.,, S. Richardson,, P. Dixon,, C. Belanger,, and P. Maxwell. 2000. Enzyme-linked immunosorbent assay for the diagnosis of bovine leukosis: comparison with the agar gel immunodiffusion test approved by the Canadian Food Inspection Agency. Can. J. Vet. Res. 64: 101– 106.

129. Sloss, M. W.,, R. L. Kemp,, and A. M. Zajac. 1994. Veterinary Clinical Parasitology. Iowa State University Press, Ames..

130. Snyder, P. S.,, J. K. Levy,, M. E. Salute,, S. P. Gorman,, P. S. Kubilis,, P. W. Smail,, and L. L. George. 2000. Performance of serologic tests used to detect heartworm infection in cats. J. Am. Vet. Med. Assoc. 216: 693– 700.

131. Sockett, D. C.,, D. J. Carr,, and M. T. Collins. 1992. Evaluation of conventional and radiometric fecal culture and a commercial DNA probe for diagnosis of Mycobacterium paratuberculosis infections in cattle. Can. J. Vet. Res. 56: 148– 153.

132. Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503– 517.

133. Swanson, E. C.,, and M. T. Collins. 1980. Use of the API 20E system to identify veterinary Enterobacteriaceae. J. Clin. Microbiol. 12: 10– 14.

134. Sweeney, R. W.,, R. H. Whitlock,, C. L. Buckley,, and P. A. Spencer. 1995. Evaluation of a commercial enzyme-linked immunosorbent assay for the diagnosis of paratubersulosis in dairy cattle. J. Vet. Diagn. Investig. 7: 488– 493.

135. Teska, J. D.,, E. B. Shotts,, and T. Hsu. 1989. Automated biochemical identification of bacterial fish pathogens using the Abbott Quantum II. J. Wildl. Dis. 25: 103– 107.

136. Ture, O.,, Y. M. Saif,, and D. J. Jackwood. 1998. Restriction fragment length polymorphism analysis of highly virulent strains of infectious bursal disease viruses from Holland, Turkey, and Taiwan. Avian Dis. 42: 470– 479.

137. Van Reeth, K. 1997. Pathogenesis and clinical aspects of a respiratory porcine reproductive and respiratory syndrome virus infection. Vet. Microbiol. 55: 223– 230.

138. Vanrompay, D.,, A. van Nerom,, R. Ducatelle,, and F. Haesebrouck. 1994. Evaluation of five immunoassays for detection of Chlamydia psittaci in cloacal and conjunctival specimens from Turkeys. J. Clin. Microbiol. 32: 1470– 1474.

139. Vincent, L. L.,, B. H. Janke,, P. S. Paul,, and P. G. Halbur. 1997. A monoclonal-antibody-based immunohistochemical method for the detection of swine influenza virus in formalin-fixed, paraffin-embedded tissues. J. Vet. Diagn. Investig. 9: 191– 195.

140. Wagstrom, E. A.,, K. J. Yoon,, C. Cook,, and J. J. Zimmerman. 2000. Diagnostic performance of a reverse transcription-polymerase chain reaction test for porcine reproductive and respiratory syndrome virus. J. Vet. Diagn. Investig. 12: 75– 78.

141. Walker, R. L.,, R. J. Greene,, and T. M. Gerig. 1990. Evaluation of four commercial anaerobic systems for identification of Eubacterium suis. J. Vet. Diagn. Investig. 2: 318– 322.

142. Wang, C.,, B. Miguel,, F. W. Austin,, and R. W. Keirs. 1999. Comparison of the immunofluorescent assay and reverse transcription-polymerase chain reaction to detect and type infectious bronchitis virus. Avian Dis. 43: 590– 596.

143. Wang, X.,, and M. I. Khan. 1999. A multiplex PCR for Massachusetts and Arkansas serotypes of infectious bronchitis virus. Mol. Cell. Probes 13: 1– 7.

144. Watts, J. L. 1989. Evaluation of the Minitek grampositive set for identification of streptococci isolated from bovine mammary glands. J. Clin. Microbiol. 27: 1008– 1010.

145. Watts, J. L. 1989. Evaluation of the Rapid Strep system for identification of gram-positive, catalase negative cocci isolated from bovine intramammary infections. J. Dairy Sci. 72: 2728– 2732.

146. Watts, J. L.,, and S. C. Nickerson. 1986. A comparison of the Staph-Ident and the Staph-Trac systems to conventional methods in the identification of staphylococci isolated from bovine udders. Vet. Microbiol. 12: 179– 187.

147. Watts, J. L.,, W. E. Owens,, and S. C. Nickerson. 1986. Evaluation of the Minitek gram-positive set for identification of staphylococci isolated from the bovine mammary gland. J. Clin. Microbiol. 23: 873– 875.

148. Watts, J. L.,, J. W. Pankey,, and S. C. Nickerson. 1984. Evaluation of the Staph-Ident and STAPH-ase systems for identification of staphylococci from bovine mammary infections. J. Clin. Microbiol. 20: 448– 452.

149. Watts, J. L.,, and R. J. Yancey. 1994. Identification of veterinary pathogens by use of commercial identification systems and new trends in antimicrobial susceptibility testing of veterinary pathogens. Clin. Microbiol. Rev. 7: 346– 356.

150. Watts, J. L.,, M. M. Chengappa,, J. R. Cole,, J. M. Cooper,, T. J. Inzana,, M. R. Plaunt,, T. R. Shrycok,, C. Thornsberry,, R. D. Walker,, and C. C. Wu. 1999. Development of In Vitro Susceptibility Testing Criteria and Quality Control Parameters for Veterinary Antimicrobial Agents. Approved Guideline. Document M37- A. , Wayne, Pa..

151. Watts, J. L.,, M. M. Chengappa,, J. R. Cole,, J. M. Cooper,, T. J. Inzana,, M. R. Plaunt,, T. R. Shrycok,, C. Thornsberry,, R. D. Walker,, and C. C. Wu. 1999. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Approved Standard. Document M31-A. , Wayne, Pa..

152. Wesley, R. D.,, W. L. Mengeling,, K. M. Lager,, D. F. Clouser,, J. G. Landgraf,, and M. L. Frey. 1998. Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5. J. Vet. Diagn. Investig. 10: 140– 144.

153. West, K.,, J. Bogdan,, A. Hamel,, G. Nayar,, P. S. Morley,, D. M. Haines,, and J. A. Ellis. 1998. A comparison of diagnostic methods for the detection of bovine respiratory syncytial virus in experimental clinical specimens. Can. J. Vet. Res. 62: 245– 250.

154. Whipple, D. L.,, D. R. Callahan,, and J. L. Jarnigan. 1991. Cultivation of Mycobacterium paratuberculosis from bovine fecal specimens and a suggested standardized procedure. J. Vet. Diagn. Investig. 3: 368– 373.

155. Whittington, R. J.,, I. Marsh,, S. McAllister,, M. J. Turner,, D. J. Marshall,, and C. A. Fraser. 1999. Evaluation of modified BACTEC 12B radiometric medium and solid media for culture of Mycobacterium avium subsp. paratuberculosis from sheep. J. Clin. Microbiol. 37: 1077– 1083.

156. Wood, M. M.,, and P. Timms. 1992. Comparison of nine antigen detection kits for diagnosis of urogenital infections due to Chlamydia psittaci in koalas. J. Clin. Microbiol. 30: 3200– 3205.

157. Zhang, S.,, W. Xue,, C. Wood,, Q. Chen,, S. Kapil,, and H. C. Minocha. 1997. Detection of bovine immunodeficiency virus antibodies in cattle by western blot assay with recombinant gag protein. J. Vet. Diagn. Investig. 9: 347– 351.

158. Zhang, Z.,, G. A. Andrews,, C. Chard-Bergstrom,, H. C. Minocha,, and S. Kapil. 1997. Application of immunohistochemistry and in situ hybridization for detection of bovine coronavirus in paraffin-embedded, formalin-fixed intestines. J. Clin. Microbiol. 35: 2964– 2965.

159. Zhou, E. M.,, M. Chan,, R. A. Heckert,, J. Riva,, and M. F. Cantin. 1998. Evaluation of a competitive ELISA for detection of antibodies against avian influenza virus nucleoprotein. Avian Dis. 42: 517– 522.

Tables

Commercial Methods in Clinical Veterinary Microbiology

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TABLE 1

Selected commercial diagnostic kits for antigen or antibody detection of veterinary viruses of major domestic animal species.

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TABLE 1

Selected commercial diagnostic kits for antigen or antibody detection of veterinary viruses of major domestic animal species.

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Table 2

Heartworm antigen tests approved for use in dogs

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Table 2

Heartworm antigen tests approved for use in dogs