
Full text loading...
Category: Clinical Microbiology
Rapid Systems and Instruments for the Identification of Bacteria, Page 1 of 2
< Previous page | Next page > /docserver/preview/fulltext/10.1128/9781555817961/9781555811891_Chap03-1.gif /docserver/preview/fulltext/10.1128/9781555817961/9781555811891_Chap03-2.gifAbstract:
Preliminary detection of bacterial pathogens and isolation in pure culture on solid media was followed by culture confirmation using one of the early-generation kits for bacterial identification. In more recent years, the development and, in some cases, very common use of direct antigen testing has revolutionized the algorithms for specimen testing which are used today in physicians’ office laboratories, community hospitals, and tertiary-care medical centers. Early antigen tests sometimes lacked the sensitivity which was offered by conventional testing. Researchers tested 345 strains of coryneform bacteria and 33 strains of Listeria spp. (representing 49 taxa). In this study, 80.9% were identified to the species level and 12.2% were identified to the genus level, with 3.7% of the strains misidentified and 3.2% of the strains not identified. Prepackaged commercially available kit systems for the identification of clinically significant gram-negative bacteria have been available for a number of years and offer the clinical microbiologist several distinct advantages over previously available methodologies. The identification systems have evolved over time and have been based on either growth dependent or enzyme-mediated substrate utilization or on the detection of specific nucleic acid sequences. A section talks about fastidious bacteria that include Haemophilus spp., Neisseria spp., Gardnerella vaginalis, Bordetella spp., and Legionella spp.
Full text loading...
BACTi Staph latex agglutination test kit (courtesy of Remel).
BACTi Staph latex agglutination test kit (courtesy of Remel).
BD LINK 2 Strep A Rapid Test (courtesy of BD Diagnostic Systems).
BD LINK 2 Strep A Rapid Test (courtesy of BD Diagnostic Systems).
RIM A.R.C. Strep A test strips with negative test (top) and positive test (bottom) results (courtesy of Remel).
RIM A.R.C. Strep A test strips with negative test (top) and positive test (bottom) results (courtesy of Remel).
BactiCard Strep with positive test results (courtesy of Remel).
BactiCard Strep with positive test results (courtesy of Remel).
API Coryne system (courtesy of bioMerieux).
API Coryne system (courtesy of bioMerieux).
IDS RapID CB Plus system (courtesy of Remel).
IDS RapID CB Plus system (courtesy of Remel).
MicroPlate test format (courtesy of Biolog).
MicroPlate test format (courtesy of Biolog).
MicroStation (courtesy of Biolog).
MicroStation (courtesy of Biolog).
API 20E, API Rapid 20E, API 20 NE, and Vitek GNI Plus (courtesy of bioMerieux).
API 20E, API Rapid 20E, API 20 NE, and Vitek GNI Plus (courtesy of bioMerieux).
BactiCard E. coli with positive test results (courtesy of Remel).
BactiCard E. coli with positive test results (courtesy of Remel).
Premier EHEC (courtesy of Meridian Diagnostics).
Premier EHEC (courtesy of Meridian Diagnostics).
ImmunoCard STAT! E. coli O157 Plus (courtesy of Meridian Diagnostics).
ImmunoCard STAT! E. coli O157 Plus (courtesy of Meridian Diagnostics).
RIM E. coli O157:H7 latex test kit (courtesy of Remel).
RIM E. coli O157:H7 latex test kit (courtesy of Remel).
Campyslide (courtesy of BD Diagnostic Systems).
Campyslide (courtesy of BD Diagnostic Systems).
ProSpecT Campylobacter Microplate Assay (courtesy of Alexon-Trend).
ProSpecT Campylobacter Microplate Assay (courtesy of Alexon-Trend).
RIM Immuno Campylobacter Microplate Assay (courtesy of Remel).
RIM Immuno Campylobacter Microplate Assay (courtesy of Remel).
RIM Immuno Campylobacter Microplate Assay tray with positive wells (courtesy of Remel).
RIM Immuno Campylobacter Microplate Assay tray with positive wells (courtesy of Remel).
Premier Platinum HpSA test (courtesy of Meridian Diagnostics).
Premier Platinum HpSA test (courtesy of Meridian Diagnostics).
Catarrhalis test disk with positive test results (courtesy of Remel).
Catarrhalis test disk with positive test results (courtesy of Remel).
Micro ID system with inoculated wells, used primarily for Enterobacteriaceae (courtesy of Remel).
Micro ID system with inoculated wells, used primarily for Enterobacteriaceae (courtesy of Remel).
API Quad-Ferm+ (courtesy of bioMerieux).
API Quad-Ferm+ (courtesy of bioMerieux).
Neisseria-Kwik tray (courtesy of Micro-Bio-Logics).
Neisseria-Kwik tray (courtesy of Micro-Bio-Logics).
BactiCard Neisseria with positive test results (courtesy of Remel).
BactiCard Neisseria with positive test results (courtesy of Remel).
Characteristics of agglutination assays for identification of S. aureus a
Characteristics of agglutination assays for identification of S. aureus a
Performance of agglutination assays for identification of S. aureus
Performance of agglutination assays for identification of S. aureus
Systems for identification of Staphylococcus spp. and other gram-positive cocci a
Systems for identification of Staphylococcus spp. and other gram-positive cocci a
Commercial serogrouping assays for identification of beta-hemolytic streptococci
Commercial serogrouping assays for identification of beta-hemolytic streptococci
Rapid, direct group A streptococcal detection tests granted waived status
Rapid, direct group A streptococcal detection tests granted waived status
Rapid, direct group A streptococcal detection tests which do not have waived status
Rapid, direct group A streptococcal detection tests which do not have waived status
Commercial identification systems for Corynebacterium spp.
Commercial identification systems for Corynebacterium spp.
Commercial systems for identification of members of the family Enterobacteriaceae and gram-negative nonmembers
Commercial systems for identification of members of the family Enterobacteriaceae and gram-negative nonmembers
Performance of Premier E. coli O157 test in the field and in resolved results after repeat testing
Performance of Premier E. coli O157 test in the field and in resolved results after repeat testing
Sensitivities, specificities, and predictive values for the detection of E. coli O157:H7 a
Sensitivities, specificities, and predictive values for the detection of E. coli O157:H7 a
Commercial systems for the identification of Haemophilus spp., Neisseria spp., and other fastidious bacteria
Commercial systems for the identification of Haemophilus spp., Neisseria spp., and other fastidious bacteria
Performance characteristics of culture, DFA, and PCR for the detection of B. pertussis in nasopharyngeal-swab specimens a
Performance characteristics of culture, DFA, and PCR for the detection of B. pertussis in nasopharyngeal-swab specimens a
Comparison of the sensitivities and specificities of the multiplex PCR-based assay and culture for B. pertussis following the resolution of discrepant results a
Comparison of the sensitivities and specificities of the multiplex PCR-based assay and culture for B. pertussis following the resolution of discrepant results a
Diagnostic methods for Legionella evaluation a
Diagnostic methods for Legionella evaluation a