Commercial Methods for Identification and Susceptibility Testing of Fungi

Donna M. Wolk; Glenn D. Roberts

doi:10.1128/9781555817961.ch9

Chapter 9 : Commercial Methods for Identification and Susceptibility Testing of Fungi

Authors: Donna M. Wolk, Glenn D. Roberts

Content Type: Reference

Publication Year: 2002

Category: Clinical Microbiology

Book DOI: 10.1128/9781555817961

Chapter DOI: 10.1128/9781555817961.ch9

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Abstract:

The methods described in this chapter are meant to represent those that are commercially available, Food and Drug Administration-approved, and published in peer-reviewed journals. The advantages of the nonautomated and automated identification systems discussed in the chapter include the fact that identification is based on databases that include a variety of substrate utilization patterns and that contain many yeast biotypes. Reproducibility was determined as results were compared in an interlaboratory study with broth macrodilution as the reference standard. In this report, the E test quality control limits were 1 dilution greater (4 dilutions overall) than those of the NCCLS broth tube macrodilution method for the following combinations: ketoconazole with C. krusei and amphotericin B and ketoconazole with C. parapsilosis. When the Premier EIA method was compared to the microimmunodiffusion (MID) test and the laboratory CF test for a total of 168 sera, including 68 from proven cases of histoplasmosis, the sensitivity of the EIA for IgG was 97%and that of the MID was 100%, and the specificities were 84 and 100%, respectively. In this study, three sera from histoplasmosis patients which were positive for the histoplasmin antigens tested negative for IgG, specific precipitins, and complement-fixing antibodies. Due to the interest in rapid, cost-effective testing for fungi, the pace and number of publications devoted to diagnostic practices in clinical mycology laboratories are growing.

Citation: Wolk D, Roberts G. 2002. Commercial Methods for Identification and Susceptibility Testing of Fungi, p 225-255. In Truant A (ed), Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555817961.ch9

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Commercial Methods for Identification and Susceptibility Testing of Fungi

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FIGURE 1

BactiCardCandida. Courtesy of Remel.

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FIGURE 1

BactiCardCandida. Courtesy of Remel.

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FIGURE 2

Uni-N/F Tek and Uni-Yeast-Tek plates (uninoculated and inoculated). Courtesy of Remel.

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FIGURE 2

Uni-N/F Tek and Uni-Yeast-Tek plates (uninoculated and inoculated). Courtesy of Remel.

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FIGURE 3

(A) Inoculation of the API 20 C (Biomerieux Vitek) strip for identification of yeast isolates. Each strip contains microcupules containing dehydrated substrates, which are rehydrated and examined to determine their ability to support the growth of yeast isolates. (B) Close-up view of substrate utilization by a yeast isolate. Positive reactions appear cloudy in comparison to the negative (0) control.

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FIGURE 3

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FIGURE 4

The RapID Yeast Plus System (Innovative Diagnostic Systems and Remel).

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FIGURE 4

The RapID Yeast Plus System (Innovative Diagnostic Systems and Remel).

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FIGURE 5

API Yeast-Ident.

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FIGURE 5

API Yeast-Ident.

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FIGURE 6

Identicult-Albicans.

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FIGURE 6

Identicult-Albicans.

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FIGURE 7

The Microscan Rapid Yeast Identification Panel. Courtesy of Dade Microscan.

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FIGURE 7

The Microscan Rapid Yeast Identification Panel. Courtesy of Dade Microscan.

References

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Tables

Commercial Methods for Identification and Susceptibility Testing of Fungi

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TABLE 1

Comparison of commercial methods for cryptococcal antigen testing

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TABLE 1

Comparison of commercial methods for cryptococcal antigen testing

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TABLE 2

Comparison of commercial methods for C. albicans antigen testing

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TABLE 2

Comparison of commercial methods for C. albicans antigen testing

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TABLE 3

Comparison of Isolator method (Wampole) with other blood culture methods and systems

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TABLE 3

Comparison of Isolator method (Wampole) with other blood culture methods and systems

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Table 4

Comparison of commercial fungal identification methods

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Table 4

Comparison of commercial fungal identification methods

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Table 5

Agreement of methods for susceptibility testing of yeasts

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Table 5

Agreement of methods for susceptibility testing of yeasts