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Chapter 17 : Proliferation Is Not Dependent on Intracellular Replication

Authors: Susanne Surman1, Glyn Morton2, Bill Keevil3, Roy Fitzgeorge3
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Affiliations: 1: London Food Water & Environmental Microbiology Laboratory, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom; 2: Department of Applied Biology, University of Central Lancashire, Preston PR1 2HE, United Kingdom; 3: Centre for Applied Microbiology and Research, Porton Down, Salisbury SP4 OJG, United Kingdom
Content Type: Monograph
Publication Year: 2002

Category: Bacterial Pathogenesis

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Proliferation Is Not Dependent on Intracellular Replication, Page 1 of 2

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Abstract:

The complexities of the relationship between and other microorganisms for the growth and survival of legionellae in the environment is still not fully understood, though it is generally believed that amoebae play an important role in the natural environment. Although strains of varying virulence are isolated from environmental sources, it is still generally accepted that intracellular replication is important for the ability of to proliferate in the natural environment. The model water system allowed the development of reproducible biofilms. A diverse but fairly constant consortium of aquatic microorganisms including fungi, bacteria, and protozoa could be maintained in this system. To investigate if legionellae could have the potential to replicate without multiplication within a protozoal host, an avirulent strain of serogroup 1 Pontiac (Corby Strain) (CAC) was added to the system. If intracellular multiplication is essential for the proliferation of in aquatic systems, then elimination of trophozoites in the model system would prevent any further growth of legionellae, and consequently numbers would decrease due to dilution by the continuous culture medium.

Citation: Surman S, Morton G, Keevil B, Fitzgeorge R. 2002. Proliferation Is Not Dependent on Intracellular Replication, p 86-89. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch17

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Legionella pneumophila Proliferation Is Not Dependent on Intracellular Replication
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Citation: Surman S, Morton G, Keevil B, Fitzgeorge R. 2002. Proliferation Is Not Dependent on Intracellular Replication, p 86-89. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch17
/content/10.1128/9781555817985.chap17.fig17-1
FIGURE 1

The effect of cycloheximide on the planktonic bacterial population of the chemostat. Total counts of the heterotrophic population (♦) and the population (■) in the planktonic phase of the continuous culture model system, before and following addition of cycloheximide (arrow), are shown 3).

10.1128/9781555817985/fig17-1_thmb.gif
10.1128/9781555817985/fig17-1.gif
Image of FIGURE 1
FIGURE 1

The effect of cycloheximide on the planktonic bacterial population of the chemostat. Total counts of the heterotrophic population (♦) and the population (■) in the planktonic phase of the continuous culture model system, before and following addition of cycloheximide (arrow), are shown 3).

Citation: Surman S, Morton G, Keevil B, Fitzgeorge R. 2002. Proliferation Is Not Dependent on Intracellular Replication, p 86-89. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch17
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/content/10.1128/9781555817985.chap17.fig17-2
FIGURE 2

The effect of cycloheximide on the biofilm bacterial population of the chemostat. Total biofilm counts (♦) and numbers of (■) in the biofilm following cycloheximide addition are shown ( = 3).

10.1128/9781555817985/fig17-2_thmb.gif
10.1128/9781555817985/fig17-2.gif
Image of FIGURE 2
FIGURE 2

The effect of cycloheximide on the biofilm bacterial population of the chemostat. Total biofilm counts (♦) and numbers of (■) in the biofilm following cycloheximide addition are shown ( = 3).

Citation: Surman S, Morton G, Keevil B, Fitzgeorge R. 2002. Proliferation Is Not Dependent on Intracellular Replication, p 86-89. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch17
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References

/content/book/10.1128/9781555817985.chap17
1. Brock, T. D. 1971. Microbial growth rates in nature. Bacteriol. Rev. 35: 39– 58.
2. Fitzgeorge, R. B.,, A. Baskerville,, M. G. Broster,, P. Hambleton,, and P. J. Dennis. 1983. Aerosol infection of animals with strains of Legionella pneumophila of different virulence: comparison with intraperitoneal and intranasal route of infection. J. Hyg. Cambridge 90: 81– 90.
3. Jepras, R. I.,, R. B. Fitzgeorge,, and A. Baskerville. 1985. A comparison of virulence of two strains of L. pneumophila based on experimental aerosol infection of guinea pigs. J. Hyg. Cambridge 95: 29– 38.
4. Lee, J. V.,, and A. A. West. 1991. Survival and growth of Legionella species in the environment. Soc. Appl. Bacteriol. Symp. Ser. 20: 121S– 129S.
5. Oleinick, N. L. 1977. Initiation and elongation of protein synthesis in growing cells: differential inhibition by cycloheximide and emetine. Arch. Biochem. Biophys. 182: 171.
6. Rogers, J.,, and C. W. Keevil. 1992. Immunogold and flouroscein immunolabelling of Legionella pneumophila within an aquatic biofilm visualized using episcopic differential interference contrast microscopy. Appl. Environ. Microbiol. 58: 2326– 2330.
7. Skaliy, P.,, and H. V. McEachern. 1979. Survival of Legionnaires' disease bacterium in water. Ann. Intern. Med. 90: 662– 663.
8. Surman, S. 1994. The integration of an avirulent Legionella pneumophila into aquatic biofilms. Ph.D. thesis, University of Central Lancashire, Lancashire, United Kingdom.
9. Tremaine, S. C.,, and A. L. Mills. 1987. Inadequacy of the eukaryote inhibitor cycloheximide in studies of protozoan grazing on bacteria at the freshwater-sediment interface. Appl. Environ. Microbiol. 53: 8, 1969– 1972.
10. Tully, M. A.,, A. Williams,, and R. B. Fitzgeorge. 1992. Transposon mutagenesis in Legionella pneumophila. 11 mutants exhibiting impaired intracellular growth within cultured alveolar macrophages and reduced virulence in vivo. Res. Microbiol. 5: 143, 481– 489.
11. Walker, J. T.,, J. Rogers,, and C. W. Keevil. 1994. An investigation of the efficacy of a bromine containing biocide on an aquatic consortium of planktonic and biofilm microorganisms including Legionella pneumophila. Biofouling 8: 47– 54.

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