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Chapter 22 : and Interleukin-12 Production: In Vitro Infection Model

Authors: Kazuto Matsunaga1, Thomas W. Klein1, Catherine Newton1, Herman Friedman1, Yoshimasa Yamamoto1
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Affiliations: 1: Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa, FL 33612-4799
Content Type: Monograph
Publication Year: 2002

Category: Bacterial Pathogenesis

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and Interleukin-12 Production: In Vitro Infection Model, Page 1 of 2

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Abstract:

Interleukin-12 (IL-12), one of the key cytokines in regulating the development of Th1 response, is a heterodimeric cytokine composed of two disulfide-linked p35 and p40 subunits. Both subunits have to be expressed within the same cell to produce biologically active p70 heterodimer. The production of IL-12 by monocytes/macrophages is induced by exposing responsive cells to a variety of microbial products such as lipopolysaccharide (LPS). The suppression of IL-12 by infection may be occurring at the level of gene transcription. The analysis of the steady-state levels of IL-12 p35 and IL-12 p40 mRNA determined by reverse transcriptase-PCR (RT-PCR) showed that infection induced the suppression of mRNA accumulation for the IL-12 p40 gene in response to LPS stimulation, but not the IL-12 p35 gene, which was constitutive in all macrophages treated. infection induced production of MCP-1 protein and up-regulated the LPS-induced production of MCP-1 protein regardless of the virulence of and its viability in the macrophages. The experimental mouse infection with resulted in certain levels of IL-12. Since IL-12 production is regulated by multiple mechanisms, including cytokines produced by other cells, it can be speculated that the suppressed IL-12 by infection may be overcome by other systems in vivo, such as the host defense system.

Citation: Matsunaga K, Klein T, Newton C, Friedman H, Yamamoto Y. 2002. and Interleukin-12 Production: In Vitro Infection Model, p 120-123. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch22

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References

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Tables

Legionella pneumophila and Interleukin-12 Production: In Vitro Infection Model
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Citation: Matsunaga K, Klein T, Newton C, Friedman H, Yamamoto Y. 2002. and Interleukin-12 Production: In Vitro Infection Model, p 120-123. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch22
/content/10.1128/9781555817985.chap22.tab22-1
TABLE 1

Effect of infection of macrophages on the protein levels of IL-12 p40/p70 production induced by LPS

10.1128/9781555817985/tab22-1_thmb.gif
10.1128/9781555817985/tab22-1.gif
Generic image for table
TABLE 1

Effect of infection of macrophages on the protein levels of IL-12 p40/p70 production induced by LPS

Citation: Matsunaga K, Klein T, Newton C, Friedman H, Yamamoto Y. 2002. and Interleukin-12 Production: In Vitro Infection Model, p 120-123. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch22
/content/10.1128/9781555817985.chap22.tab22-2
TABLE 2

Effect of virulent (LP-V) infection of macrophages on the protein levels of IL-l, IL-6, and IL-10 production induced by LPS

10.1128/9781555817985/tab22-2_thmb.gif
10.1128/9781555817985/tab22-2.gif
Generic image for table
TABLE 2

Effect of virulent (LP-V) infection of macrophages on the protein levels of IL-l, IL-6, and IL-10 production induced by LPS

Citation: Matsunaga K, Klein T, Newton C, Friedman H, Yamamoto Y. 2002. and Interleukin-12 Production: In Vitro Infection Model, p 120-123. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch22

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