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Chapter 6 : Iron Requirements of and Acquisition of Iron by

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Abstract:

This chapter discusses the current understanding of the relationship between and the metal iron. It summarizes the work of a number of laboratories that demonstrated the importance of iron for , and highlights recent advances toward uncovering mechanisms of iron acquisition. The mutant displayed a 42% reduction in hemin binding, confirming that potentiates hemin acquisition by . Within U937 cells, NU216 and its allelic equivalent NU216R were approximately 100-fold more sensitive than the wild type to treatment with desferoxamine, confirming that they are defective for intracellular iron acquisition. The EDDA-hypersensitive mutant NU208 was also dramatically impaired for replication in U937 cells, and its infectivity defect was exacerbated by treatment of the macrophages with desferoxamine. A reconstruction of the NU208 mutation confirmed that the iron acquisition and infectivity defects were due to the transposon insertion and not a spontaneous second-site mutation. Sequence analysis demonstrated that the transposon disruption lies within a gene that is highly similar to the cytochrome c maturation gene, . is generally recognized for its role in the heme export step of cytochrome biogenesis. Quantitative infection assays demonstrated that NU229 was impaired ca. 80-fold in intracellular growth. Reconstruction of the mutant by allelic exchange proved that the defect was due to the inactivation of frgA and not a spontaneous second-site mutation. Subsequently, trans-complementation of the mutation demonstrated that the infectivity defect was directly due to the loss of FrgA.

Citation: Cianciotto N, Kurtz S, Krcmarik K, Mody S, Prasad U, Robey M, Salerno J, Viswanathan V. 2002. Iron Requirements of and Acquisition of Iron by , p 31-37. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch6
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FIGURE 1

Potential iron acquisition pathways of . See text for details.

Citation: Cianciotto N, Kurtz S, Krcmarik K, Mody S, Prasad U, Robey M, Salerno J, Viswanathan V. 2002. Iron Requirements of and Acquisition of Iron by , p 31-37. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch6
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References

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