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Chapter 60 : Analysis of Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies

Authors: Junko Amemura-Maekawa1, Fumiaki Kura1, Haruo Watanabe1, Fumio Gondaira2, Jun-ichi Sugiyama2
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Affiliations: 1: Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; 2: Denka Seiken Co., Ltd., Gosen-shi, Niigata 959-1836, Japan
Content Type: Monograph
Publication Year: 2002

Category: Bacterial Pathogenesis

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Analysis of Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies, Page 1 of 2

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Abstract:

serogroup 1 is the serogroup of species most frequently isolated from infected patients in Japan and all over the world. The recent findings suggest that one of the most discriminative epidemiological methods is pulsed-field gel electrophoresis (PFGE). To develop the method of epidemiological surveillance, PFGE and monoclonal antibodies were used for the analysis of 27 clinical and 20 environmental serogroup 1 isolates in Japan. Researchers reported five anti- serogroup 1 monoclonal antibodies that were specific for serogroup 1 but showed different reactivities, previously. Monoclonal antibody-sensitized latex was prepared and the latex agglutination test on slide plate was performed. There was a close correlation between monoclonal antibody subtyping and PFGE analysis. The result by the two analyses suggested that the clinical isolates, the isolates from cooling towers, and the isolates from hot springs in Japan formed the distinctive genetically or antigenic clusters.

Citation: Amemura-Maekawa J, Kura F, Watanabe H, Gondaira F, Sugiyama J. 2002. Analysis of Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies, p 302-304. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch60

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Pulsed-Field Gel Electrophoresis
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Latex Agglutination Test
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Legionella pneumophila
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Analysis of Legionella pneumophila Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies
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Citation: Amemura-Maekawa J, Kura F, Watanabe H, Gondaira F, Sugiyama J. 2002. Analysis of Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies, p 302-304. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch60
/content/10.1128/9781555817985.chap60.fig60-1
FIGURE 1

Cluster dendrogram of Japanese L. pneumophila serogroup 1 isolates generated from Sfil restriction fragments separated by PFGE and analyzed with the program Molecular Analyst (Bio-Rad). Designations of strains were indicated along with original ones by providers. Source is as follows: E, environmental; HS, hot spring; CT, cooling tower; C, clinical.

10.1128/9781555817985/fig60-1_thmb.gif
10.1128/9781555817985/fig60-1.gif
Image of FIGURE 1
FIGURE 1

Cluster dendrogram of Japanese L. pneumophila serogroup 1 isolates generated from Sfil restriction fragments separated by PFGE and analyzed with the program Molecular Analyst (Bio-Rad). Designations of strains were indicated along with original ones by providers. Source is as follows: E, environmental; HS, hot spring; CT, cooling tower; C, clinical.

Citation: Amemura-Maekawa J, Kura F, Watanabe H, Gondaira F, Sugiyama J. 2002. Analysis of Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies, p 302-304. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch60
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References

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1. De Zoysa, A. S.,, and T. G. Harrison. 1999. Molecular typing of Legionella pneumophila sero group 1 by pulsed-field gel electrophoresis with Sfil and comparison of this method with restriction fragment-length polymorphism analysis. J. Med. Microbiol. 48:269– 278.
2. Hunter, P. R.,, and M. A. Gaston. 1988. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J. Clin. Microbiol. 26: 2465– 2466.
3. Gondaira, F.,, and J. Sugiyama. 1996. Subserogrouping of 49 Legionella pneumophila serogroup 1 strains with monoclonal antibodies by slide latex agglutination method and its usefulness for epidemiologic study. Kansenshogaku Zasshi. 70: 673– 680.
4. Izumiya, H.,, J. Terajima,, A. Wada,, Y. Inagaki,, K.-I. Itoh,, K. Tamura,, and H. Watanabe. 1997. Molecular typing of enterohemorrhagic Escherichia coli 0157:H7 isolates in Japan by using pulsed-field gel electrophoresis. J. Clin. Microbiol. 35: 1675– 1680.
5. Pruckler, J. M.,, L. A. Mermel,, R. F. Benson,, C. Giorgio,, P. K. Cassiday,, R. F. Breiman,, C. G. Whitney,, and B. S. Fields. 1995. Comparison of Legionella pneumophila isolates by arbitrarily primed PCR and pulsed-field gel electrophoresis: analysis from seven epidemic investigations. J Clin. Microbiol. 33: 2872– 2875.
6. Schoonmaker, D.,, T. Heimberger,, and G. Birkhead. 1992. Comparison of ribotyping and restriction enzyme analysis using pulsed-field gel electrophoresis for distinguishing Legionella pneumophila isolates obtained during a nosocomial outbreak. J. Clin. Microbiol. 30: 1491– 1498.
7. Sugiyama, J.,, F. Gondaira,, J. Matsuda,, M. Saga,, and Y. Terada. 1987. New method for serological typing of Vibrio cholerae O: 1 using a monoclonal antibody-sensitized latex agglutination test. Microbiol. Immunol. 31: 387– 391.

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