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Analysis of Legionella pneumophila Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies, Page 1 of 2
< Previous page Next page > /docserver/preview/fulltext/10.1128/9781555817985/9781555812300_Chap60-1.gif /docserver/preview/fulltext/10.1128/9781555817985/9781555812300_Chap60-2.gifAbstract:
Legionella pneumophila serogroup 1 is the serogroup of Legionella species most frequently isolated from infected patients in Japan and all over the world. The recent findings suggest that one of the most discriminative epidemiological methods is pulsed-field gel electrophoresis (PFGE). To develop the method of epidemiological surveillance, PFGE and monoclonal antibodies were used for the analysis of 27 clinical and 20 environmental L. pneumophila serogroup 1 isolates in Japan. Researchers reported five anti-L. pneumophila serogroup 1 monoclonal antibodies that were specific for serogroup 1 but showed different reactivities, previously. Monoclonal antibody-sensitized latex was prepared and the latex agglutination test on slide plate was performed. There was a close correlation between monoclonal antibody subtyping and PFGE analysis. The result by the two analyses suggested that the clinical isolates, the isolates from cooling towers, and the isolates from hot springs in Japan formed the distinctive genetically or antigenic clusters.