Chapter 14 : Biosynthetic Pathways Related to Cell Structure and Function

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The spiral morphology of appears to confer an advantage to the bacterium in the viscous gastric mucus, since its spiral forms move more effectively in media with high viscosity than more conventional rod-shaped organisms. Peptidoglycans are the stress-bearing structures of bacteria that maintain the integrity of the cell wall and the shape of the bacterium. There is an overall decrease in monomers as the bacteria age, while the percentage of dimers, "anhydro" residues, and dipeptide monomers increases. The lack of detectable trimers or tetramers in murein indicates that cross-linking does not occur between three or more glycan chains, whereas cross-linking between three or four glycan chains is detectable in . The presence of pentapeptide as the main fraction of muropeptides indicates that it possesses little carboxypeptidase activity. On the basis of protein sequence homologies, the genome of appears to code for homologs of all the enzymes involved in the cytoplasmic synthesis of the disaccharide pentapeptide, starting with the synthesis of UDP-N-acetylmuramic acid and finishing with UDP-disaccharide pentapeptide linked to an undecaprenyl lipid carrier. The second step in building the murein sacculus is the polymerization reaction, accompanied by the insertion of the newly made material into the existing peptidoglycan (PG) layer. Analysis of the genome shows the presence of genes coding for all the enzymes of the biosynthetic pathway leading to the disaccharide pentapeptide, which is the basic building block of murein.

Citation: Krishnamurthy P, Phadnis S, Dunn B, Deloney C, Rosenthal R. 2001. Biosynthetic Pathways Related to Cell Structure and Function, p 159-166. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch14
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Figure 1

General composition and age changes in PG. As cells age from early to late log phase, the composition of their PG changes. The shaded region represents the amino acids that are removed from the PG in late log phase, essentially resulting in a PG with increased dipeptides.

Citation: Krishnamurthy P, Phadnis S, Dunn B, Deloney C, Rosenthal R. 2001. Biosynthetic Pathways Related to Cell Structure and Function, p 159-166. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch14
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1. Alm, R. A.,, L. S. Ling,, D. T. Moir,, B. L. King,, E. D. Brown,, P. C. Doig,, D. R. Smith,, B. Noonan,, B. C. Guild,, B. L. dejonge,, G. Caraiel,, P. J. Tummino,, A. Caruso,, M. Uria-Nickelsen,, D. M. Mills,, C. Ives,, R. Gibson,, D. Merberg,, S. D. Mills,, Q. Jiang,, D. E. Taylor,, G. F. Vovis,, and T. J. Trust. 1999. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397: 176 180.
2. Benaissa, M.,, P. Babin,, N. Quellard,, L. Pezennec,, Y. Cenatiempo,, and J. L. Fauchere. 1996. Changes in Helicobacter pylori ultrastructure and antigens during conversion from the bacillary to the coccoid form. Infect. Immun. 64: 2331 2335.
3. Betzner, A. S.,, and W. Keck. 1989. Molecular cloning, overexpression and mapping of the sit gene encoding the soluble lytic transglycosylase of Escherichia coli. Mol. Gen. Genet. 219: 489 491.
4. Blasco, B.,, A. G. Pisabarro,, and M. A. de Pedro. 1988. Peptidoglycan biosynthesis in stationary-phase cells of Escherichia coli. J. Bacteriol. 170: 5224 5228.
5. Blaser, M. J. 1992. Hypotheses on the pathogenesis and natural history of Helicobacter pylori-induced inflammation. Gastroenterology 102: 720 727.
6. Blaser, M. J. 1993. Helicobacter pylori: microbiology of a 'slow' bacterial infection. Trends Microbiol. 1: 255 260.
7. Blaser, M. J. 1994. Helicobacter pylori phenotypes associated with peptic ulceration. Scand. ]. Gastroenterol. Suppl. 205: 1 5.
8. Bode, G.,, F. Mauch,, and P. Malfertheiner. 1993. The coccoid forms of Helicobacter pylori. Criteria for their viability. Epidemiol. Infect. 111: 483 490.
9. Braun, V.,, H. Gnirke,, U. Henning,, and K. Rehn. 1973. Model for the structure of the shape-maintaining layer of the Escherichia coli cell envelope. J. Bacteriol. 114: 1264 1270.
10. Burman, L. G.,, and J. T. Park. 1983. Changes in the composition of Escherichia coli murein as it ages during exponential growth. J. Bacteriol. 155: 447 453.
11. Cao, P.,, M. S. McClain,, M. H. Forsyth,, and T. L. Cover. 1998. Extracellular release of antigenic proteins by Helicobacter pylori. Infect. Immun. 66: 2984 2986.
12. Cooper, S. 1991. Synthesis of the cell surface during the division cycle of rod-shaped, gram-negative bacteria. Microbiol. Rev. 55: 649 674.
13. Costa, K.,, G. Bacher,, G. Allmaier,, M. G. Dominguez-Bello,, L. Engstrand,, P. Falk,, M. A. de Pedro,, and F. Garcia-del Portillo. 1999. The morphological transition of Helicobacter pylori cells from spiral to coccoid is preceded by a substantial modification of the cell wal l. J. Bacteriol. 181: 3710 3715.
14. DeLoney, C. R.,, and N. L. Schiller. 1999. Competition of various beta-lactam antibiotics for the major penicillin-binding proteins of Helicobacter pylori: antibacterial activity and effects on bacterial morphology. Antimicrob. Agents Chemother 43: 2702 2709.
15. DeLoney, C. R.,, and N. L. Schiller. Characterization of an in vitro-selected amoxicillin-resistant strain of Helicobacter pylori. Antimicrob. Agents Chemother. 44: 3368 3373.
16. Doig, P.,, B. Dejonge,, R. A. Aim,, E. D. Brown,, M. Uria-Nickelsen,, B. Noonan,, S. D. Mills,, P. Tummino,, G. Carmel,, B. C. Guild,, D. T. Moir,, G. F. Vovis,, and T. J. Trust. 1999. Helicobacter pylori physiology predicted from genomic comparison of two strains. Microbiol. Mol. Biol. Rev. 63: 675 707.
17. Dore, M. P.,, D. Y. Graham,, and A. R. Sepulveda. 1999. Different penicillin-binding protein profiles in amoxicillin-resistant Helicobacter pylori. Helicobacter 4: 154 161.
18. Eaton, K. A.,, D. R. Morgan,, and S. Krakowka. 1989. Campylobacter pylori virulence factors in gnotobiotic piglets. Infect. Immun. 57: 1119.
19. Engel, H.,, B. Kazemier,, and W. Keck. 1991. Murein-metabolizing enzymes from Escherichia coli: sequence analysis and controlled overexpression of the sit gene, which encodes the soluble lytic transglycosylase. J. Bacteriol. 173: 6773 6782.
20. Forman, D.,, D. G. Newell,, F. Fullerton,, J. W. Yarnell,, A. R. Stacey,, N. Wald,, and F. Sitas. 1991. Association between infection with Helicobacter pylori and risk of gastric cancer: evidence from a prospective investigation. Br. Med. J. 302: 1302 1305.
21. Forsberg, C. W.,, M. K. Rayman,, J. W. Costerton,, and R. A. MacLeod. 1972. Isolation, characterization, and ultrastructure of the peptidoglycan layer of a marine pseudomonad. J. Bacteriol. 109: 895 905.
22. Georgopapadakou, N. H. 1993. Penicillin-binding proteins and bacterial resistance to beta-lactams. Antimicrob. Agents Chemother. 37: 2045 2053.
23.. Ghuysen, J. M. 1977. CellSurface Reviews. Elsevier and North Holland Publishing Co., Amsterdam, The Netherlands.
24. Ghuysen, J. M. 1988. Bacterial active-site serine penicillin-interactive proteins and domains: mechanism, structure, and evolution. Rev. Infect. Dis. 10: 726 732.
25. Ghuysen, J. M. 1991. Serine beta-lactamases and penicillin-binding proteins. Annu. Rev. Microbiol. 45: 37 67.
26. Ghuysen, J. M. 1994. Molecular structures of penicillin-binding proteins and beta-lactamases. Trends Microbiol. 2: 372 380.
27. Ghuysen, J. M.,, J. M. Frere,, M. Leyh-Bouille,, M. Nguyen-Disteche,, J. Coyette,, J. Dusart,, B. Joris,, C. Duez,, O. Dideberg,, and P. Charlier. 1984. Bacterial wall peptidoglycan, DD-peptidases and beta-lactam antibiotics. Scand. J. Infect. Dis. Suppl. 42: 17 37.
28. Ghuysen, J. M.,, and R. Hakenbeck. 1994. Bacterial Cell Wall. Elsevier Biomedical Press, Amsterdam, The Netherlands.
29. Glauner, B. 1988. Separation and quantification of neuropeptides with high-performance liquid chromatography. Anal. Biochem. 172: 451 464.
30. Glauner, B.,, and J. V. Holtje. 1990. Growth pattern of the murein sacculus of Escherichia coli. J. Biol. Chem. 265: 18988 18996.
31. Glauner, B.,, J. V. Holtje,, and U. Schwarz. 1988. The composition of the murein of Escherichia coli. J. Biol. Chem. 263: 10088 10095.
32. Gmeiner, J. 1980. Identification of peptide-cross-linked trisdisaccharide peptide trimers in murein of Escherichia coli. J. Bacteriol. 143: 510 512.
33.Goffin, C, and J. M. Ghuysen. 1998. Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs. Microbiol. Mol. Biol. Rev. 62: 10791093.
34. Goodell, E. W. 1985. Recycling of murein by Escherichia coli. J. Bacteriol. 163: 305 310.
35. Goodell, E. W.,, and C. F. Higgins. 1987. Uptake of cell wall peptides by Salmonella typhimurium and Escherichia coli. J. Bacteriol. 169: 3861 3865.
36. Goodell, E. W.,, and U. Schwarz. 1985. Release of cell wall peptides into culture medium by exponentially growing Escherichia coli. J. Bacteriol. 162: 391 397.
37. Greenway, D. L.,, and H. R. Perkins. 1985. Turnover of the cell wall peptidoglycan during growth of Neisseria gonorrhoeae and Escherichia coli. Relative stability of newly synthesized material. J. Gen. Microbiol. 131: 253 263.
38. Harris, A. G.,, S. L. Hazell,, and A. G. Netting. 2000. Use of digoxigenin-labeled ampicillin in the identification of penicillin-binding proteins in Helicobacter pylori. J . Antimicrob. Chemother. 45: 591 598.
39. Harz, H.,, K. Burgdorf,, and J. V. Holtje. 1990. Isolation and separation of the glycan strands from murein of Escherichia coli by reversed-phase high-performance liquid chromatography. Anal. Biochem. 190: 120 128.
40. Hazell, S. L.,, A. Lee,, L. Brady,, and W. Hennessy. 1986 Campylobacter pyloridis and gastritis association with intercellular spaces and adaptation to an environment of mucus as important factors in colonization of the gastric epithelium. J. Infect. Dis. 153: 658.
41. Heilmann, H. D. 1972. On the peptidoglycan of the cell walls of Pseudomonas aeruginosa. Eur. ]. Biochem. 31: 456 463.
42. Holtje, J. V. 1998. Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli. Microbiol. Mol. Biol. Rev. 62: 181 203.
43. Holtje, J. V.,, D. Mirelman,, N. Sharon,, and U. Schwarz. 1975. Novel type of murein transglycosylase in Escherichia coli. J. Bacteriol. 124: 1067 1076.
44. Ikeda, F.,, Y. Yokota,, Y. Mine,, and M. Tatsuta. 1990. Activity of cefixime against Helicobacter pylori and affinities for the penicillin-binding proteins. Antimicrob. Agents Chemother. 34: 2426 2428.
45. Ishino, F.,, and M. Matsuhashi. 1981. Peptidoglycan synthetic enzyme activities of highly purified penicillin-binding protein 3 in Escherichia coli: a septum-forming reaction sequence. Biochem. Biophys. Res. Commun. 101: 905 911.
46. Kato, K.,, S. Iwata,, H. Suginaka,, K. Namba,, and S. Kotani. 1976. Chemical structure of the peptidoglycan of Vibrio parahaemolyticus A55 with special reference to the extent of interpeptide cross-linking. Biken. J. 19: 139 150.
47. Katz, W.,, and H. H. Martin. 1970. Peptide crosslinkage in cell wall murein of Proteus mirabilis and its penicillin-induced unstable L-form. Biochem. Biophys. Res. Commun. 39: 744 749.
48. Kreig, N. R. (ed.). 1984. Bergey's Manual of Systematic Bacteriology. Williams &C Wilkins, Baltimore, Md. 11: 119 211
49. Krishnamurthy, P.,, B. E. Dunn,, and S. H. Phadnis. 2000. Unpublished data.
50. Krishnamurthy, P.,, B. E. Dunn,, and S. H. Phadnis. 2000. Unpublished data.
51. Krishnamurthy, P.,, M. H. Parlow,, R. S. Rosenthal,, B. L. deJonge,, S. H. Phadnis,, and B. E. Dunn. 2000. Unpublished data.
52. Krishnamurthy, P.,, M. H. Parlow,, J. Schneider,, S. Burroughs,, C. Wickland,, N. B. Vakil,, B. E. Dunn,, and S. H. Phadnis. 1999. Identification of a novel penicillin-binding protein from Helicobacter pylori. J. Bacteriol. 181: 5107 5110.
53. Mai, U. E. H.,, M. Shahamat,, and R. R. Colwell,. 1991. Survival of Helicobacter pylori in the aquatic environment. In H. Menge,, M. Gregor,, G. N. J. Tytgat,, B. J. Marshall,, and C. A. M. McNulty (ed.), Helicobacter pylori 1990. Springer-Verlag, Berlin, Germany.
54. Martin, J. P.,, J. Fleck,, M. Mock,, and J. M. Ghuysen. 1973. The wall peptidoglycans of Neisseria perflava, Moraxella glucidolytica, Pseudomonas alcaligenes, and Proteus vulgaris strain P18. Eur. J. Biochem. 38: 301 306.
55. Mittl, P. R.,, L. Luthy,, P. Hunziker,, and M. G. Grutter. 2000. The cysteine-rich protein A from Helicobacter pylori is a beta-lactamase. J. Biol. Chem. 275: 17693 17699.
56. Nanninga, N. 1991. Cell division and peptidoglycan assembly in Escherichia coli. Mol. Microbiol. 5: 791 795.
57. Nilius, M.,, A. Strohle,, G. Bode,, and P. Malfertheiner. 1993. Coccoid like forms (CLF) of Helicobacter pylori. Enzyme activity and antigenicity. Zentralbl. Bakteriol. 280: 259 272.
58. Nomura, A.,, G. N. Stemmermann,, P. H. Chyou,, I. Kato,, G. I. Perez-Perez,, and M. J. Blaser. 1991. Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii. N. Engl. J. Med. 325: 1132 1136.
59. Parsonnet, J.,, G. D. Friedman,, D. P. Vandersteen,, Y. Chang,, J. H. Vogelman,, N. Orentreich,, and R. K. Sibley. 1991. Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl.]. Med. 325: 1127 1131.
60. Parsonnet, J.,, S. Hansen,, L. Rodriguez,, A. B. Gelb,, R. A. Warnke,, E. Jellum,, N. Orentreich,, J. H. Vogelman,, and G. D. Friedman. 1994. Helicobacter pylori infection and gastric lymphoma. n. Engl. J. Med. 330: 1267 1271.
61. Pisabarro, A. G.,, M. A. de Pedro,, and D. Vazquez. 1985. Structural modifications in the peptidoglycan of Escherichia coli associated with changes in the state of growth of the culture. J. Bacteriol. 161: 238 242.
62. Rogers, H. J.,, and H. R. Perkins (ed.). 1980. Microbial Cell Walls and Membranes. Chapman and Hall, London, England.
63. Rosenthal, R. S.,, R. M. Wright,, and R. K. Sinha. 1980. Extent of peptide cross-Unking in the peptidoglycan of Neisseria gonorrhoeae. Infect. Immun. 28: 867 875.
64. Schleifer, K. H.,, and O. Kandler. 1972. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36: 407 477.
65. Seidl, P. H.,, and K. H. Schleifer (ed.). 1986. Biological Properties of Peptidoglycan. Walter De Gruyter, Berlin, Germany.
66. Shockman, G. D.,, and J. F. Barrett. 1983. Structure, function, and assembly of cell walls of gram-positive bacteria. Annu. Rev. Microbiol 37: 501 527.
67. Spratt, B. G. 1975. Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12. Proc. Natl. Acad. Sci. USA 72: 2999 3003.
68. Spratt, B. G.,, and K. D. Cromie. 1988. Penicillin-binding proteins of gram-negative bacteria. Rev. Infect. Dis. 10: 699 711.
69.Takebe, 1. 1965. Extent of cross-linkage in the murein sacculus of Escherichia coli B cell wall. Biochim. Biophys. Acta 101: 124126.
70. Tomb, J.-F.,, O. White,, A. R. Kerlavage,, R. A. Clayton,, G. G. Sutton,, R. D. Fleischmann,, K. A. Ketchum,, H. P. Klenk,, S. Gill,, B. A. Dougherty,, K. Nelson,, J. Quackenbush,, L. Zhou,, E. F. Kirkness,, S. Peterson,, B. Loftus,, D. Richardson,, R. Dodson,, H. G. Khalak,, A. Glodek,, K. McKenney,, L. M. Fitzegerald,, N. Lee,, M. D. Adams,, and J. C. Venter. 1997. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 38: 8539 547.
71.. Tomioka, S.,, T. Nikaido,, T. Miyakawa,, and M. Matsuhashi. 1983. Mutation of the N-acetylmuramyl-L-alanine amidase gene of Escherichia coli K12. J. Bacterial. 156: 463 465.
72. Van Heijenoort, J.,, C. Parquet,, B. Flouret,, and Y. Van Heijenoort. 1975. Envelope-bound-N-acetylmuramyl-L-alanine amidase of Escherichia coli K12. Purification and properties of the enzyme. Eur. J. Biochem. 58: 611 619.
73. Weidel, W.,, and H. Peizer. 1964. Bagshaped macromolecules: a new outlook on bacterial cell walls. Adv. Enzymol. 26: 193 232.
74. Winter, A. J.,, W. Katz,, and H. H. Martin. 1971. Murein (peptidoglycan) structure of Vibrio fetus. Comparison of a venereal and an intestinal strain. Biochim. Biophys. Acta 244: 58 64.


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Table 1

Structure of peptidoglycan isolated from H. strains SP1, 84–183, NCTC 11637 and MC 4100

Citation: Krishnamurthy P, Phadnis S, Dunn B, Deloney C, Rosenthal R. 2001. Biosynthetic Pathways Related to Cell Structure and Function, p 159-166. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch14
Generic image for table
Table 2

List of putative genes that are involved in PG biosynthesis

Citation: Krishnamurthy P, Phadnis S, Dunn B, Deloney C, Rosenthal R. 2001. Biosynthetic Pathways Related to Cell Structure and Function, p 159-166. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch14
Generic image for table
Table 3

26695 ORFs that show sequence motifs of PBPs

Citation: Krishnamurthy P, Phadnis S, Dunn B, Deloney C, Rosenthal R. 2001. Biosynthetic Pathways Related to Cell Structure and Function, p 159-166. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch14

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