Chapter 28 : Genetic Exchange

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has a recombinational, or panmictic, population structure, which is indicative of frequent genetic exchange among strains. Horizontal gene exchange may occur via three classical mechanisms: natural transformation, conjugation, and transduction. The aim of this chapter is to review what is known about each of these mechanisms of exchange in the context of . Transformation is defined as the mechanism by which exogenous DNA is taken up by bacteria and the DNA becomes heritable. In a study, a chromosomal antibiotic resistance marker was transferred from one strain to another. Conjugation may provide an important method for performing genetic manipulations in strains resistant to transformation. The use of conjugation as a mechanism for DNA delivery may have the advantage of bypassing restriction barriers that could inhibit genetic exchange by natural transformation. Several potential barriers exist to genetic exchange for . Although the information regarding genetic exchange in continues to expand, there remain many unanswered questions. Finally, although there is one study that indicates gene transfer can occur in vivo based on strains isolated from a human subject, in vivo animal studies need to be performed to shed light on the significance of all three methods for genetic exchange in an environment that recapitulates human gastric mucosa. Ultimately, elucidating the mechanisms of genetic exchange in will lead to a better understanding of the immense diversity that exists, as well as further development of genetic tools for the study of .

Citation: Israel D. 2001. Genetic Exchange, p 313-319. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch28
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Generic image for table
Table 1

Characteristics of mechanisms for genetic exchange

Citation: Israel D. 2001. Genetic Exchange, p 313-319. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch28
Generic image for table
Table 2

predicted proteins with putative roles in transformation or conjugation

Citation: Israel D. 2001. Genetic Exchange, p 313-319. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch28

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