Chapter 23 : Solving Genetic Puzzles

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The author found the logic of Koch's approach for proving that a particular organism caused a particular disease particularly appealing, as well as the description of figuring out how to make single colonies on a potato slice. The author's postdoctoral project was on the site-specific excision of bacteriophage lambda. Mark Shulman, who had preceded her in the lab, had developed a lambda derivative that allowed an intramolecular excision reaction. The aim was to reconstitute the reaction in vitro; Howard Nash was using similar approaches to look at the integration reaction. Some progress was made, and the researchers found out something about the in vivo reaction as well. The researchers wanted to understand what sort of cellular substrates Lon degraded and how proteolysis is used by the cell to regulate gene expression. The ultimate test was the demonstration that suspected targets were rapidly degraded but were stabilized in mutants.

Citation: Gottesman S. 2000. Solving Genetic Puzzles, p 179-185. In Atlas R (ed), Many Faces, Many Microbes. ASM Press, Washington, DC. doi: 10.1128/9781555818128.ch23

Key Concept Ranking

Microbial Genetics
Bacterial Genetics
Missense Mutation
Gene Expression
ATP-Dependent Protease
Serratia marcescens
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Citation: Gottesman S. 2000. Solving Genetic Puzzles, p 179-185. In Atlas R (ed), Many Faces, Many Microbes. ASM Press, Washington, DC. doi: 10.1128/9781555818128.ch23
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1. Gottesman, S.,, S. Wickner,, and M. R. Maurizi. 1997. Protein quality control: triage by chaperones and proteases. Genes Dev. 11: 815 823.
2. Sledjeski, D. D.,, A. Gupta,, and S. Gottesman. 1996. The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli. EMBO J. 15: 3993 4000.
3. Wickner, S.,, S. Gottesman,, D. Skowyra,, J. Hoskins,, K. McKenney,, and M. Maurizi. 1994. A molecular chaperone, ClpA, functions like DnaK and DnaJ. Proc. Natl. Acad. Sci. USA 91: 12218 12222.
4. Maurizi, M. R.,, W. P. Clark,, S. -H Kim,, and S. Gottesman. 1990. CIpP represents a unique family of serine proteases. J. Biol. Chem. 265: 12546 12552.
5. Gottesman, S.,, C. Squires,, E. Pichersky,, M. Carrington,, M. Hobbs,, J. S. Mattick,, B. Dalrymple,, H. Kuramitsu,, T. Shiroza,, T. Foster,, W. P. Clark,, B. Ross,, C. Squires,, and M. R. Maurizi. 1990. Conservation of the regulatory subunit for the Clp ATP-dependent protease in prokaryotes and eukaryotes. Proc. Natl. Acad. Sci. USA 87: 3513 3517.
6. Torres-Cabassa, A. S.,, and S. Gottesman. 1987. Capsule synthesis in Escherichia coli K-12 is regulated by proteolysis. J. Bacteriol. 169: 981 989.
7. Mizusawa, S.,, and S. Gottesman. 1983. Protein degradation in Escherichia coli: The lon gene controls the stability of SulA protein. Proc. Natl. Acad. Sci. USA 80: 358 362.

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