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ANA DETECTION BY IIF ON HEp-2 CELLS

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(A) Homogeneous nuclear staining: antibodies to DNA (double or single stranded), to deoxynucleoproteins, or to histone proteins. Note the fluorescence of the chromosomal regions of the mitotic cells.

Citation: Folds J, Normansell D. 1999. ANA DETECTION BY IIF ON HEp-2 CELLS, In Pocket Guide to Clinical Immunology. ASM Press, Washington, DC.
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(B) Speckled nuclear staining: mixed coarse and fine speckling. Absence of staining in the nucleoli and chromosomal regions of mitotic cells suggests antibodies to RNP.

Citation: Folds J, Normansell D. 1999. ANA DETECTION BY IIF ON HEp-2 CELLS, In Pocket Guide to Clinical Immunology. ASM Press, Washington, DC.
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(C) Nucleolar staining: coarse speckling within the nucleoli suggests antibodies to 4-6S RNA.

Citation: Folds J, Normansell D. 1999. ANA DETECTION BY IIF ON HEp-2 CELLS, In Pocket Guide to Clinical Immunology. ASM Press, Washington, DC.
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(D) Centromere staining: antibodies to kinetochore proteins. Note the characteristic staining of the mitotic cells.

Citation: Folds J, Normansell D. 1999. ANA DETECTION BY IIF ON HEp-2 CELLS, In Pocket Guide to Clinical Immunology. ASM Press, Washington, DC.
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(E) Speckled nuclear staining: antibodies to the Smith antigen. Mixed coarse and fine speckling, no nucleolar speckling, often seen in combination with speckling patterns as illustrated in plate B.

Citation: Folds J, Normansell D. 1999. ANA DETECTION BY IIF ON HEp-2 CELLS, In Pocket Guide to Clinical Immunology. ASM Press, Washington, DC.
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(F) Finely speckled nuclear and cytoplasmic staining: antibodies to SSA/Ro. The chromosomal regions of mitotic cells are not stained.

Citation: Folds J, Normansell D. 1999. ANA DETECTION BY IIF ON HEp-2 CELLS, In Pocket Guide to Clinical Immunology. ASM Press, Washington, DC.
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(G) Mixed coarse and fine nuclear speckling, including nucleolar speckling (coarse) and cytoplasmic speckling (fine): antibodies to SSA/Ro and SSB/La. The nucleolar staining is no more intense than that of the rest of the nuclear staining; the chromosomal regions of mitotic cells are not stained.

Citation: Folds J, Normansell D. 1999. ANA DETECTION BY IIF ON HEp-2 CELLS, In Pocket Guide to Clinical Immunology. ASM Press, Washington, DC.
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(H) Fine nuclear speckling, including nucleoli (often intense) and chromosomal regions of mitotic cells: antibodies to Scl-70 (topoisomerase I).

Citation: Folds J, Normansell D. 1999. ANA DETECTION BY IIF ON HEp-2 CELLS, In Pocket Guide to Clinical Immunology. ASM Press, Washington, DC.
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(I) Diffuse cytoplasmic speckling, usually negative for nuclear staining: antibodies to Jo-1. Note that this patient also shows anti-nucleolar staining.

Citation: Folds J, Normansell D. 1999. ANA DETECTION BY IIF ON HEp-2 CELLS, In Pocket Guide to Clinical Immunology. ASM Press, Washington, DC.
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(J) Staining of the kinetoplast of by antibodies to dsDNA.

Citation: Folds J, Normansell D. 1999. ANA DETECTION BY IIF ON HEp-2 CELLS, In Pocket Guide to Clinical Immunology. ASM Press, Washington, DC.
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