Section 4 : Specimen Management Summary Tables

Table 15a

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15b

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15c

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15d

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15e

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15f

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15g

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15h

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15i

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15j

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15k

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15l

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15m

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15n

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 15o

Bacteriology and mycology specimen collection guidelines ^a

^a EtOH, ethanol; RT, room temperature; i.v., intravenous; AFB, acid-fast bacilli; CSF, cerebrospinal fluid; BAP, blood agar plate; CHOC, chocolate agar; BHI, brain heart infusion; PVA, polyvinyl alcohol fixative.

^b All specimens should be transported in leakproof plastic bags having a separate compartment for the requisition.

Table 16

Specimen management for infrequently encountered organisms (4, 25) ^a

^a SPS, sodium polyanethol sulfonate; AO, acridine orange; FA, fluorescent antibody stain; CSF, cerebrospinal fluid; IFA, indirect fluorescent antibody stain.

^b Laboratory safety hazard.

Table 17

Specimen guide for virus isolation ^a

^a CNS, central nervous system; HIV, human immunodeficiency virus; CMV, cytomegalovirus; DFA, direct fluorescent antibody; EBV, Epstein-Barr virus; THR, throat; LES, lesion; URN, urine; CSF, cerebrospinal fluid; FEC, feces.

^b Virus isolation: method of choice for diagnosis. ‘‘Enterovirus’’ includes the historical designation of echovirus and coxsackie A and B viruses.

^c Serology: method of choice for diagnosis.

^d Includes western equine, eastern equine, St. Louis, and California encephalitis viruses.

^e Antigen / nucleic acid detection methods available

Table 18

Virology specimen collection guidelines ^a

^a BAL, bronchoalveolar lavage; CSF, cerebral spinal fluid; VTM, viral transport medium; RT, room temperature; CMV, cytomegalovirus; HSV, herpes simplex virus; EBV, Epstein-Barr virus; HIV-1, human immunodeficiency virus type 1; LCMV, lymphocytic choriomeningitis virus; EIA, enzyme immunoassay; RSV, respiratory synctial virus; ELISA, enzyme-linked immunosorbent assay; VZV, varicella-zoster virus.

^b Dacron-, rayon-, or cotton-tipped swabs with plastic or aluminum shafts are acceptable; calcium alginate swabs or swabs with wooden shafts are not acceptable.

Table 19

Parasitology: anatomic sites containing diagnostic stages ^a

^a BLD, blood; CNS, central nervous system; GI, gastrointestinal; L/ S, liver / spleen; LN, lymph node; MUS, muscle; RBC, red blood cell; WBC, white blood cell.

^b Includes L. tropica, L. mexicana complex, and L. braziliensis complex.

^c Wuchereria brancrofti and Brugia malayi.

^d Skin snips for M. streptocerca and O. volvulus.

^e Naegleria fowleri.

^f Clonorchis sinensis.

^g Cysticerci.

^h IncludesTrypanosoma brucei gambiense and T. brucei rhodesiense.

Table 20a

Parasitology specimen collection guidelines ^a

^a RT, room temperature; CSF, cerebrospinal fluid; CNS, central nervous system; FOR, 10% formalin; MIF, merthiolate–iodine-formalin; SAF, sodium acetate-formalin; PVA, polyvinyl alcohol.

^b Collect 10 ml of heparinized blood for buffy coat concentration.

^c Additional smears obtained 6, 12, or 24 h after admission may be necessary.

^d CSF is specimen of choice for patients infected >6 months.

Table 20b

Parasitology specimen collection guidelines ^a

^a RT, room temperature; CSF, cerebrospinal fluid; CNS, central nervous system; FOR, 10% formalin; MIF, merthiolate–iodine-formalin; SAF, sodium acetate-formalin; PVA, polyvinyl alcohol.

^b Collect 10 ml of heparinized blood for buffy coat concentration.

^c Additional smears obtained 6, 12, or 24 h after admission may be necessary.

^d CSF is specimen of choice for patients infected >6 months.

Table 20c

Parasitology specimen collection guidelines ^a

^a RT, room temperature; CSF, cerebrospinal fluid; CNS, central nervous system; FOR, 10% formalin; MIF, merthiolate–iodine-formalin; SAF, sodium acetate-formalin; PVA, polyvinyl alcohol.

^b Collect 10 ml of heparinized blood for buffy coat concentration.

^c Additional smears obtained 6, 12, or 24 h after admission may be necessary.

^d CSF is specimen of choice for patients infected >6 months.

Table 20d

Parasitology specimen collection guidelines ^a

^a RT, room temperature; CSF, cerebrospinal fluid; CNS, central nervous system; FOR, 10% formalin; MIF, merthiolate–iodine-formalin; SAF, sodium acetate-formalin; PVA, polyvinyl alcohol.

^b Collect 10 ml of heparinized blood for buffy coat concentration.

^c Additional smears obtained 6, 12, or 24 h after admission may be necessary.

^d CSF is specimen of choice for patients infected >6 months.

Table 20e

Parasitology specimen collection guidelines ^a

^a RT, room temperature; CSF, cerebrospinal fluid; CNS, central nervous system; FOR, 10% formalin; MIF, merthiolate–iodine-formalin; SAF, sodium acetate-formalin; PVA, polyvinyl alcohol.

^b Collect 10 ml of heparinized blood for buffy coat concentration.

^c Additional smears obtained 6, 12, or 24 h after admission may be necessary.

^d CSF is specimen of choice for patients infected >6 months.

Table 20f

Parasitology specimen collection guidelines ^a

^a RT, room temperature; CSF, cerebrospinal fluid; CNS, central nervous system; FOR, 10% formalin; MIF, merthiolate–iodine-formalin; SAF, sodium acetate-formalin; PVA, polyvinyl alcohol.

^b Collect 10 ml of heparinized blood for buffy coat concentration.

^c Additional smears obtained 6, 12, or 24 h after admission may be necessary.

^d CSF is specimen of choice for patients infected >6 months.

Table 20g

Parasitology specimen collection guidelines ^a

^a RT, room temperature; CSF, cerebrospinal fluid; CNS, central nervous system; FOR, 10% formalin; MIF, merthiolate–iodine-formalin; SAF, sodium acetate-formalin; PVA, polyvinyl alcohol.

^b Collect 10 ml of heparinized blood for buffy coat concentration.

^c Additional smears obtained 6, 12, or 24 h after admission may be necessary.

^d CSF is specimen of choice for patients infected >6 months.