Chemotactic Signal Transduction in Escherichia coli and Salmonella typhimurium

Charles D. Amsler; Philip Matsumura

doi:10.1128/9781555818319.ch6

Chapter 6 : Chemotactic Signal Transduction in Escherichia coli and Salmonella typhimurium

Authors: Charles D. Amsler¹, Philip Matsumura²

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Affiliations: 1: Department of Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294-1170; 2: Department of Microbiology and Immunology (M/C 790), University of Illinois at Chicago, Chicago, Illinois 60612-7344

Content Type: Monograph

Publication Year: 1995

Category: Microbial Genetics and Molecular Biology

Book DOI: 10.1128/9781555818319

Chapter DOI: 10.1128/9781555818319.ch6

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Abstract:

Bacterial chemotaxis is an important model for two-component regulatory systems. The flow of information through the chemotactic signal transduction pathway and the proteins responsible for it have been characterized in great detail. The most detailed knowledge of the chemotactic signal transduction pathway comes from studies of the closely related enteric bacteria Escherichia coli and Salmonella typhimurium. The information pathway in these species serves as the model to which all other species are usually compared. Consequently, the chapter focuses exclusively on chemotaxis in E. coli and S. typhimurium. The components of the signal pathway in these two species are virtually interchangeable. The transmembrane receptors discussed in the chapter detect compounds in the periplasm and transmit this information to the cytoplasm, but separate transport proteins are required for uptake. The chapter provides an overview of the function of components and the pathway as a whole while calling attention to some recent advances. Although chemotaxis is probably the most thoroughly understood of all signal transduction pathways, significant gaps remain in our understanding of it. Computer-based quantitative assays of chemotactic behavior are becoming available and will facilitate quantitative analysis of chemotaxis in whole free-swimming cells.

Citation: Amsler C, Matsumura P. 1995. Chemotactic Signal Transduction in Escherichia coli and Salmonella typhimurium, p 89-103. In Hoch J, Silhavy T (ed), Two-Component Signal Transduction. ASM Press, Washington, DC. doi: 10.1128/9781555818319.ch6

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Chemotactic Signal Transduction in Escherichia coli and Salmonella typhimurium

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FIGURE 1

Overall model of chemotactic signal transduction. Abbreviations: AL, CheAL; As, CheAs; B, CheB; BB, flagellar basal body; CCW, counterclockwise flagellar rotation; CW, clockwise flagellar rotation; FF, flagellar filament; FM, flagellar motor;MCP, methyl-accepting chemotaxis protein; P, phosphate; R, CheR; W, CheW; Y, CheY; Z, CheZ.

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FIGURE 1

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FIGURE 2

Flagellar patterns during smooth swimming and tumble behaviors.

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FIGURE 2

Flagellar patterns during smooth swimming and tumble behaviors.

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FIGURE 3

Swimming behavior in homogeneous versus heterogeneous environments. Smooth swimming and tumbles as in Fig. 2 , with length of arrows indicating the duration of smooth swimming. Bacteria swim smoothly longer when their environment is getting better than when it is unchanging. There is no difference in smooth swimming duration between homogeneous environments and gradients when the environment is getting worse.

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FIGURE 3

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