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Chapter 23 : Holistic Perspective on the Escherichia coli Hemolysin

Author: Rodney A. Welch1
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Affiliations: 1: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin 53706
Content Type: Monograph
Publication Year: 1994

Category: Microbial Genetics and Molecular Biology; Bacterial Pathogenesis

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Abstract:

This chapter reviews the knowledge of the prototype of the RTX family of pore-forming toxins, the hemolysin, and proposes that new efforts be made to study the significance and roles of the exotoxins, together with those of endotoxins, as an integrated synergistic system of multiple virulence factors. The fundamental approach taken by biochemists and cell biologists interested in understanding the role in pathogenesis of a bacterial virulence factor such as an exoprotein has been to purify the exoprotein and observe its activity on different substrates, cells, and hosts. The RTX-associated diseases include pertussis, juvenile periodontitis, pneumonia, urinary tract infections, and wound infections. The epidemiological evidence for an association between RTX toxin production and isolates that cause disease is strong for all of the organisms except i, , and . The solubilization of hemolysin aggregates by a chaotropic agent such as urea causes an increase in the specific lytic activity of the hemolysin. The erythrocytes transform into echinocytes, in which gross cytoskeletal or membrane irregularities result in rounding of the cell and the formation of multiple teardrop-shaped projections from the cell surface. The cellular transformation is similar to that caused by the treatment of erthrocytes by a calcium ionophore. The motivation for covering the older literature in the chapter is to stimulate thought, experimentation, and discussion about the significance of the ever-present lipopolysaccharide (LPS) molecules on the structure and activity of the hemolysin.

Citation: Welch R. 1994. Holistic Perspective on the Escherichia coli Hemolysin, p 351-364. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch23

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Holistic Perspective on the Escherichia coli Hemolysin
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Citation: Welch R. 1994. Holistic Perspective on the Escherichia coli Hemolysin, p 351-364. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch23
/content/10.1128/9781555818340.chap23.fig23-1
Figure 1

After a 5-min treatment with the hemolysin, sheep erythrocytes were fixed and visualized by scanning electron microscopy ( ). The photograph shows the loss of the concave cellular structure typical of erythrocytes and the formation of multiple teardrop-shaped projections from the cell surface. Bar, 2 μm. The photograph was the gift of Sally Jorgensen, University of Minnesota.

10.1128/9781555818340/fig23-1_thmb.gif
10.1128/9781555818340/fig23-1.gif
Image of Figure 1
Figure 1

After a 5-min treatment with the hemolysin, sheep erythrocytes were fixed and visualized by scanning electron microscopy ( ). The photograph shows the loss of the concave cellular structure typical of erythrocytes and the formation of multiple teardrop-shaped projections from the cell surface. Bar, 2 μm. The photograph was the gift of Sally Jorgensen, University of Minnesota.

Citation: Welch R. 1994. Holistic Perspective on the Escherichia coli Hemolysin, p 351-364. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch23
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Tables

Holistic Perspective on the Escherichia coli Hemolysin
[com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/author/10.1128/9781555818340.chap23-1,webId=/content/author/10.1128/9781555818340.chap23-1,properties={foaf_givenname=Rodney A., foaf_name=Rodney A. Welch, foaf_surname=Welch, pub_isAffiliatedWith=[com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/affiliation/10.1128/9781555818340.chap23-aff1]]}]]
Citation: Welch R. 1994. Holistic Perspective on the Escherichia coli Hemolysin, p 351-364. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch23
/content/10.1128/9781555818340.chap23.tab23-1
Table 1

Selected features of toxins from the RTX toxin branch of the RTX exoprotein family

The predicted pi, based on the predicted amino acid sequence of the toxin gene product.

PMNs, polymorphonuclear leukocytes.

A weak hemolytic activity is observed.

No hemolytic activity is observed.

10.1128/9781555818340/tab23-1_thmb.gif
10.1128/9781555818340/tab23-1.gif
Generic image for table
Table 1

Selected features of toxins from the RTX toxin branch of the RTX exoprotein family

The predicted pi, based on the predicted amino acid sequence of the toxin gene product.

PMNs, polymorphonuclear leukocytes.

A weak hemolytic activity is observed.

No hemolytic activity is observed.

Citation: Welch R. 1994. Holistic Perspective on the Escherichia coli Hemolysin, p 351-364. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch23

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