1887

Chapter 3 : Transposon Tn3, 1973 to 1980

Authors: Fred Heffron1, Ronald E. Gill2
VIEW AFFILIATIONS HIDE AFFILIATIONS
Affiliations: 1: Department of Microbiology and Immunology, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland, Oregon 97201-3098; 2: Department of Microbiology and Immunology, Campus Box B175, 4200 East Ninth Avenue, Denver, Colorado 80262
Content Type: Monograph
Publication Year: 1994

Category: Microbial Genetics and Molecular Biology; Bacterial Pathogenesis

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Preview this chapter:
Preview Magnify
Zoom in
Zoomout

Transposon Tn3, 1973 to 1980, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555818340/9781555810825_Chap03-1.gif /docserver/preview/fulltext/10.1128/9781555818340/9781555810825_Chap03-2.gif

Abstract:

This chapter reviews the work on transposable drug resistance, specifically, the ampicillin resistance transposon Tn3, that was carried out in Stanley Falkow's laboratory. It starts by describing the events that occurred in the fall of 1973 and ends with the departure of Ron Gill from the laboratory in 1980. The researchers assumed that the gene for ampicillin-resistant (Ap) would show observable cross-hybridization, if there were any DNA identity. The observation that the same β-lactamase gene and some of its flanking DNA was moving from plasmid to plasmid seemed clear, but one could imagine many different mechanisms. At that time it was not clear whether this phenomenon was peculiar to Tn3 or could be generalized to other antibiotic resistance genes. Several of the antibiotic-resistant isolates were compared by DNA-DNA heteroduplex analysis. Different fractions from the gradient were used to transform by the CaCl method, and recombinants were found at an intermediate density. The insertions were mapped by heteroduplex analysis and electron microscopy following cleavage with EcoRI provided by Herb Boyer (EcoRI cleaved the target molecule once). The electron microscopic data and the results of restriction enzyme analysis were both consistent with the interpretation that complementation of these mutants resulted in a single insertion into the target plasmid.

Citation: Heffron F, Gill R. 1994. Transposon Tn3, 1973 to 1980, p 43-53. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch3
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555818340.chap3
1. Anderson, E. S.,, and M. J. Lewis. 1965. Drug resistance and its transfer in Salmonella typhimurium. Nature (London) 206: 579– 583.
2. Barth, P.,, and M. J. Grinter. 1974. Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamide. J. Bacteriol. 120: 618– 630.
3. Berg, D. E.,, J. Daviues,, B. Allet,, and J. D. Rochaix. 1975. Transposition of R factor genes to bacteriophage lambda. Proc. Natl. Acad. Sci. USA 72: 3628– 3632.
4. Carbon, J.,, T. E. Shenk,, and P. Berb. 1975. Biochemical procedure for production of small deletions in simian virus 40 DNA. Proc. Natl. Acad. Sci. USA 72: 1392– 1396.
5. Chan, R. K.,, D. Botstein,, T. Watanabe,, and U. Ogata. 1972. Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate. Virology 50: 883– 898.
6. Cohen, S. N.,, A. C. Chang,, and L. Hsu. 1972. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc. Natl. Acad. Sci. USA 69: 2110– 2114.
7. Crosa, J. H.,, D. J. Brenner,, and S. Falkow. 1973. Use of single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacterid. 115: 904– 911.
8. Datta, N. 1971. Prevalence of extrachromosomal drug resistance. R factors in Escherichia coli. Ann. N. Y. Acad. Sci. 182: 59– 64.
9. Datta, N.,, and R. W. Hedges. 1971. Compatibility groups among fi-R factors. Nature (London) 234: 222– 223.
10. Datta, N.,, and P. Kontomichalou. 1965. Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae. Nature (London) 208: 239– 241.
11. Datta, N.,, and M. H. Richmond. 1966. The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli. Biochem. J. 98: 204– 209.
11a. Dougan, G.,, M. Saul,, A. Twigg,, R. Gill,, and D. Sherratt. 1979. Polypeptides expressed in Escherichia coli K-12 minicells by transposition elements Tni and Tni. J. Bacteriol. 138: 48– 54.
12. Falkow, S.,, L. P. Elwell,, J. de Graaff,, F. Heffron,, and L. Mayer,. A possible model for the development of plasmid-mediated penicillin resistance in the gonococcus, p. 120– 134. In R. D. Catterall, and C. S. Nicol (ed.), Sexually Transmitted Diseases.
13. Gill, R. E.,, F. Heffron,, G. Dougan,, and S. Falkow. 1978. Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tni. J. Bacteriol. 136: 742– 756.
14. Gill, R. E.,, F. Heffron,, and S. Falkow. 1979. Identification of the protein encoded by the transposable element Tn3 which is required for its transposition. Nature (London) 282: 797– 801.
15. Gorai, A. P.,, F. Heffron,, S. Falkow,, R. W. Hedges,, and N. Datta. 1979. Electron microscope heteroduplex studies of sequence relationships among plasmids of the W incompatibility group. Plasmid 2: 485– 492.
16. Hedges, R. W.,, and A. E. Jacob. 1974. Transposition of ampicillin resistance from RP4 to other replicons. Mot. Gen. Genet. 132: 31– 40.
17. Heffron, F.,, P. Bedinger,, J. J. Champoux,, and S. Falkow. 1977. Deletions affecting the transposition of an antibiotic resistance gene. Proc. Natl. Acad. Sci. USA 74: 702– 706.
18. Heffron, F.,, C. Rubens,, and S. Falkow. 1975. Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc. Natl. Acad. Sci. USA 72: 3623– 3627.
19. Heffron, F.,, C. Rubens,, and S. Falkow. 1977. Transposition of a plasmid deoxyribonucleic acid sequence that mediates ampicillin resistance: identity of laboratory-constructed plasmids and clinical isolates. J. Bacteriol. 129: 530– 533.
19a. Heffron, F.,, M. So,, and B. J. McCarthy. 1978. In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites. Proc. Natl. Acad. Sci. USA 75: 6012– 6016.
20. Heffron, F.,, R. W. Sublett,, R. W. Hedges,, A. Jacob,, and S. Falkow. 1975. Origin of the TEM-beta-lactamase gene found on plasmids. J. Bacteriol. 122: 250– 256.
21. Kleckner, N.,, D. F. Barker,, D. G. Ross,, and D. Botstein. 1978. Properties of the translocatable tetracycline-resistance elements Tn/0 in Escherichia coli and bacteriophage lambda. Genetics 90: 427– 461.
22. Kleckner, N.,, R. K. Chan,, B. K. Tye,, and D. Botstein. 1975. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J. Mol. Biol. 97: 561– 575.
23. Kleckner, N.,, K. Reichardt,, and D. Botstein. 1979. Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10. J. Mol. Biol. 127: 89– 115.
24. Kleckner N.,, J. Roth,, and D. Botstein. 1977. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. J. Mol. Biol. 116: 125– 159.
25. Kopecko, D. J.,, J. Brevet,, and S. N. Cohen. 1976. Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids. J. Mol. Biol. 108: 333– 360.
26. Kopecko, D. J.,, and S. N. Cohen. 1975. Site specific recA-independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc. Natl. Acad. Sci. USA 72: 1373– 1377.
27. Mat hew, M.,, and R. W. Hedges. 1976. Analytical isoelectrofocusing of R factor-determined β-lactamases: correlation with plasmid compatibility. J. Bacteriol. 125: 713– 718.
28. Nisen, P. D.,, D. J. Kopecko,, J. Chou,, and S. N. Cohen. 1977. Site-specific DNA deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3). J. Mol. Biol. 117: 975– 978.
29. Ross, D. G.,, J. Swan,, and N. Kleckner. 1979. Physical structures of Tn10-promoted deletions and inversions: role of 1400 bp inverted repetions. Cell 16: 721– 731.
30. Rubens, C.,, F. Heffron,, and S. Falkow. 1975. Transposition of a plasmid deoxyribonucleic acid sequence that mediates ampicillin resistance: independence from host rec functions and orientation of insertion. J. Bacteriol. 128: 425– 434.
31. Saedler, H.,, J. Besemer,, B. Kemper,, B. N. Rosenwirth,, and P. Starlinger. 1972. Insertion mutations in the control region of the Gal operon of E. coli. I. Biological characterization of the mutations. Mol. Gen. Genet. 115: 258– 265.
32. Shapiro, J. A. 1979. Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proc. Natl. Acad. Sci. USA 76: 1933– 1937.
33. Shapiro, J. A. 1969. Mutations caused by the insertion of genetic material into the galactose operon of Escherichia coli. J. Mol. Biol. 40: 93– 105.
34. So, M.,, F. Heffron,, and S. Falkow. 1978. Method for the genetic labeling of cryptic plasmids. J. Bacteriol. 133: 1520– 1523.
35. Tye, B. K.,, R. K. Chan,, and D. Botstein. 1974. Packaging of an oversize transduction genome by Salmonella phage P22. J. Mol. Biol. 85: 485– 500.

Back to top
This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error