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Chapter 7 : Current Laboratory Methods for the Diagnosis of Tuberculosis
Authors:
Leonid B. Heifets1,
Robert C. Good2
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Affiliations:
1: National Jewish Center for Immunology and Respiratory Medicine, 1400 Jackson Street, Denver, Colorado 80206;
2: Centers for Disease Control and Prevention, 1600 Clifton Road, N.E., Mailstop C-09, Atlanta, Georgia 30333
Content Type: Monograph
Publication Year:
1994
Category: Fungi and Fungal Pathogenesis; Clinical Microbiology
Book DOI:
10.1128/9781555818357
Chapter DOI:
10.1128/9781555818357.ch7
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Current Laboratory Methods for the Diagnosis of Tuberculosis, Page 1 of 2
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Abstract:
This chapter presents a broad picture of activities in a diagnostic mycobacteriology laboratory. It addresses the methodology currently available in most laboratories as well as the techniques that require further standardization and therefore have been implemented in only a few laboratories, mostly in conjunction with research and development. It provides short descriptions of these new methods in order to review their possible application in clinical laboratories. It also gives a condensed description of biosafety practices. In order to present a comprehensive review of all activities that take place in a clinical mycobacteriology laboratory, the chapter focuses on biosafety in the mycobacteriology laboratory, and specimen collection, smear examination, and inoculation of primary culture media. It then describes methods for identification of Mycobacterium tuberculosis. Finally, the chapter talks about drug susceptibility testing for detection of initial and acquired drug resistance.
Citation:
Heifets L, Good R.
1994.
Current Laboratory Methods for the Diagnosis of Tuberculosis,
p 85-110.
In
Bloom B (ed),
Tuberculosis.
ASM Press, Washington, DC.
doi: 10.1128/9781555818357.ch7
Figures
Current Laboratory Methods for the Diagnosis of Tuberculosis
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Citation:
Heifets L, Good R.
1994.
Current Laboratory Methods for the Diagnosis of Tuberculosis,
p 85-110.
In
Bloom B (ed),
Tuberculosis.
ASM Press, Washington, DC.
doi: 10.1128/9781555818357.ch7
/content/10.1128/9781555818357.chap7.fig7-1
Figure 1
M. tuberculosis in a smear from a concentrated sputum specimen. Photography by N. Mor. (A and B) Ziehl-Nielsen stain; Olympus microscope model BH2 with l00x objective. Total magnification, ×1,125. (C) Auramine O stain; fluorescence microscope, Olympus model BH2 with 40x objective. Total magnification, × 1, 440. (D) Auramine O stain; fluorescence microscope, model Leitz-Wetzlar with 40x objective. Total magnification, × 2, 000.
10.1128/9781555818357/fig7-1_thmb.gif
10.1128/9781555818357/fig7-1.gif
Figure 1
M. tuberculosis in a smear from a concentrated sputum specimen. Photography by N. Mor. (A and B) Ziehl-Nielsen stain; Olympus microscope model BH2 with l00x objective. Total magnification, ×1,125. (C) Auramine O stain; fluorescence microscope, Olympus model BH2 with 40x objective. Total magnification, × 1, 440. (D) Auramine O stain; fluorescence microscope, model Leitz-Wetzlar with 40x objective. Total magnification, × 2, 000.
Citation:
Heifets L, Good R.
1994.
Current Laboratory Methods for the Diagnosis of Tuberculosis,
p 85-110.
In
Bloom B (ed),
Tuberculosis.
ASM Press, Washington, DC.
doi: 10.1128/9781555818357.ch7
/content/10.1128/9781555818357.chap7.fig7-2
Figure 2
M. tuberculosis in cultures. Ziehl-Nielsen stain; Olympus microscope model BH2 with l00x objective. Total magnification, × 1,125. Photography by N. Mor. (A) Smear from a culture grown on 7H11 agar; (B) "cord" formation in 7H12 broth; (C and D) intracellular growth in human-monocyte-derived macrophages.
10.1128/9781555818357/fig7-2_thmb.gif
10.1128/9781555818357/fig7-2.gif
Figure 2
M. tuberculosis in cultures. Ziehl-Nielsen stain; Olympus microscope model BH2 with l00x objective. Total magnification, × 1,125. Photography by N. Mor. (A) Smear from a culture grown on 7H11 agar; (B) "cord" formation in 7H12 broth; (C and D) intracellular growth in human-monocyte-derived macrophages.
Citation:
Heifets L, Good R.
1994.
Current Laboratory Methods for the Diagnosis of Tuberculosis,
p 85-110.
In
Bloom B (ed),
Tuberculosis.
ASM Press, Washington, DC.
doi: 10.1128/9781555818357.ch7
References
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