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Chapter 35 : The Chromosome Map of Streptomycescoelicolor A3 (2)

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Abstract:

Genetic mapping of the chromosome of A3(2) began with the analysis of haploid recombinants selected from matings. A physical map of the chromosome has recently been constructed, using pulsed-field gel electrophoresis of AseI and DraI fragments. The genetic map was found to be reasonably congruent with the physical map over the well-marked quadrants. It was also apparent that the silent regions are indeed long stretches of DNA. It certainly appears at present that wild-type carry considerable stretches of DNA that presumably has selective value in natural habitats-otherwise it would not have persisted-but has only subtle effects on phenotype when deleted in laboratory variants. The arrangement of genes in the well-marked segments seems to be extensively conserved even between that are not very closely related, as in the well-known case of K-12 and LT2, or in pseudomonads, which show extensive map similarities for groups of genes of primary metabolism but not for segments determining specialized functions peculiar to individual species.

Citation: Hopwood D, Kieser H, Kieser T. 1993. The Chromosome Map of Streptomycescoelicolor A3 (2), p 497-504. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch35
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Figure 1

Combined genetic and physical map of the S. A3(2) chromosome. The physical map for the restriction enzymes AseI and ( ) is shown as outer and inner bold circles, respectively. Restriction fragment sizes are given in Table 2 (which also includes the sizes of the unmapped fragments). Evidence on which the order of fragments is based (including linking clones) is in Kieser et al. ( ). On the inside are markers located physically but not genetically; their positions are indicated at the midpoint of the restriction fragment to which they have been assigned (sometimes a notional fragment). The outside circle shows the genetic map, corrected by expansion of the 3 o'clock silent region (between the loci of and by one-third (see text). The spacing of loci on the genetic map is approximate only. Dotted lines connect physically mapped loci (shown in large type) to their approximate positions on the physical map; the three continuous lines indicate the precise positions of IS-A, IS-B, and the cluster (which contain sites). Braces indicate groups of loci whose order has not been determined. Loci outside of short dotted lines have not been ordered with respect to loci inside such lines.

Citation: Hopwood D, Kieser H, Kieser T. 1993. The Chromosome Map of Streptomycescoelicolor A3 (2), p 497-504. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch35
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References

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Tables

Generic image for table
Table 1

Mapped genes of S. A3(2)

Citation: Hopwood D, Kieser H, Kieser T. 1993. The Chromosome Map of Streptomycescoelicolor A3 (2), p 497-504. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch35
Generic image for table
Table 2

Restriction fragments of the chromosome of S. ?145

Citation: Hopwood D, Kieser H, Kieser T. 1993. The Chromosome Map of Streptomycescoelicolor A3 (2), p 497-504. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch35

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