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Category: Bacterial Pathogenesis; Microbial Genetics and Molecular Biology
Temperate Bacteriophages, Page 1 of 2
< Previous page | Next page > /docserver/preview/fulltext/10.1128/9781555818388/9781555810535_Chap57-1.gif /docserver/preview/fulltext/10.1128/9781555818388/9781555810535_Chap57-2.gifAbstract:
Bacillus subtilis is susceptible to a variety of bacteriophages, both virulent (lytic) and temperate (capable of forming lysogens). Mutations in the operator or in the repressor sequence alter binding in ways that agree with evidence that the repressor interacts with the canonical operator sequence with specific amino acid-nucleotide interactions. An unusual incompatibility phenomenon involving prophage SPO2 and the Staphylococcus aureus plasmid pC194, which is often used in B. subtilis, has been described in this chapter. The amino acid sequence of the SPO2 DNA polymerase has some similarity to those of the carboxyl ends of E. coli DNA polymerase I and coliphage T7 DNA polymerase in the region that is believed to encode the DNA-binding domain and to various other motifs in the Klenow fragment of E. coli DNA polymerase I and other polymerases. Genes for thymidylate synthetase have been cloned from ø3T (gene thyP3) and from p11 (gene thyP11). The cloned genes carry their own promoters and are expressed in B. subtilis and E. coli. The particular cytidylic acids affected differ from phage to phage and lie within the sequences corresponding to one or another restriction endonuclease target. Sequence comparisons of multispecific DNA methyltransferases are being studied to shed light on the evolution of these enzymes. The widespread distribution of group V defective prophages in strains of Bacillus has led to speculation that the prophages may carry out some essential function in the lives of the bacteria.
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.?105 operator region. The six known sites to which the ?105 repressor binds are indicated by dark bars. The PM and PR promoters, the gene that encodes the repressor protein (c ? 105), and the first gene of the late operon (orf-3) are shown. The direction of each operator sequence is indicated by short arrows beneath the bars. See reference 127 for further details.
.?105 operator region. The six known sites to which the ?105 repressor binds are indicated by dark bars. The PM and PR promoters, the gene that encodes the repressor protein (c ? 105), and the first gene of the late operon (orf-3) are shown. The direction of each operator sequence is indicated by short arrows beneath the bars. See reference 127 for further details.
Characteristics of B. subtilis phages a
Characteristics of B. subtilis phages a
DNA methyltransferases of group III phages a
DNA methyltransferases of group III phages a