Chapter 106 : Monitoring Autoimmune Reactivity within the Retina

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The identification of immune reactivity against self components is a key element in the characterization of autoimmunity and autoimmune diseases. This autoimmune reactivity is most frequently monitored by the detection of specific autoantibodies or the presence of T-cell reactivity to self peptides. Numerous studies have now demonstrated that distinct profiles of autoantibodies are seen in clinically distinct autoimmune syndromes, such as systemic lupus erythematosus, myositis, and insulin-dependent diabetes mellitus. Moreover, the autoantigens are targets of an adaptive immune response.

Citation: Hooks J, Chan C, Sen H, Nussenblatt R, Detrick B. 2016. Monitoring Autoimmune Reactivity within the Retina, p 998-1003. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch106
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Image of FIGURE 1

Immunofluorescence staining for antiretinal antibodies. Frozen sections of normal mouse retina were incubated with “normal” nonreactive human sera (A) or with sera from a patient with AIR (B). Both sera were tested at a 1:160 dilution. Note reactivity with several marked areas of the retina: retinal ganglia cell layer (RGC), INL, ONL, and RPE.

Citation: Hooks J, Chan C, Sen H, Nussenblatt R, Detrick B. 2016. Monitoring Autoimmune Reactivity within the Retina, p 998-1003. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch106
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1. Hooks JJ, Nagineni CN, Hooper LC, Hayashi K, Detrick B . 2008. IFN-β provides immuno-protection in the retina by inhibiting ICAM-1 and CXCL9 in retinal pigment epithelial cells. J Immunol 180 : 3789 3796.[PubMed].[CrossRef]
2. Nussenblatt RB, Whitcup SM . 2003. Uveitis: Fundamentals and Clinical Practice, 3rd ed. Mosby, Philadelphia, PA.
3. Resnikoff S, Pascolini D, Etya'ale D, Kocur I, Pararajasegaram R, Pokharel GP, Mariotti SP . 2004. Global data on visual impairment in the year 2002. Bull World Health Organ 82 : 844 851.[CrossRef].[PubMed]
4. Forooghian F, Macdonald IM, Heckenlively JR, Héon E, Gordon LK, Hooks JJ, Detrick B, Nussenblatt RB . 2008. The need for standardization of antiretinal antibody detection and measurement. Am J Ophthalmol 146 : 489 495.[CrossRef]
5. Chan CC, Mochizuki M . 1999. Sympathetic ophthalmia: an autoimmune ocular inflammatory disease. Springer Semin Immunopathol 21 : 125 134.[PubMed].[CrossRef]
6. Chan CC, Nussenblatt RB, Fujikawa LS, Palestine AG, Stevens G Jr, Parver LM, Luckenbach MW, Kuwabara T . 1986. Sympathetic ophthalmia. Immunopathological findings. Ophthalmology 93 : 690 695.[PubMed].[CrossRef]
7. Kuiper JJ, Van Setten J, Ripke S, Van 't Slot R, Mulder F, Missotten T, Baarsma GS, Francioli LC, Pulit SL, De Kovel CG, Ten Dam-Van Loon N, Den Hollander AI, Huis in het Veld P, Hoyng CB, Cordero-Coma M, Martín J, Llorenç V, Arya B, Thomas D, Bakker SC, Ophoff RA, Rothova A, De Bakker PI, Mutis T, Koeleman BP . 2014. A genome-wide association study identifies a functional ERAP2 haplotype associated with birdshot chorioretinopathy. Hum Mol Genet 23 : 6081 6087.[CrossRef].[PubMed]
8. Kuiper JJ, Emmelot ME, Rothova A, Mutis T . 2013. Interleukin-17 production and T helper 17 cells in peripheral blood mononuclear cells in response to ocular lysate in patients with birdshot chorioretinopathy. Mol Vis 19 : 2606 2614.[PubMed]
9. Caspi RR . 2011. Understanding autoimmune uveitis through animal models. The Friedenwald Lecture. Invest Ophthalmol Vis Sci 52 : 1872 1879.[CrossRef].[PubMed]
10. Detrick B, Hooks JJ . 2010. Immune regulation in the retina. Immunol Res 47 : 153 161.[CrossRef].[PubMed]
11. Detrick B, Hooks JJ, Nussenblatt R, . 2014. Infections associated with retinal autoimmunity, p 943 957. In Rose NR, Shoenfeld Y (ed), Infection and Autoimmunity, 2nd ed. Elsevier, Amsterdam, The Netherlands.
12. Hooks JJ, Detrick B, Nussenblatt R, . 2004. Infections associated with retinal autoimmunity, p 691 700. In Shoenfeld Y, Rose NR (ed), Infection and Autoimmunity. Elsevier, Amsterdam, The Netherlands.
13. Detrick B, Lee MT, Chin MS, Hooper LC, Chan CC, Hooks JJ . 2008. Experimental coronavirus retinopathy (ECOR): retinal degeneration susceptible mice have an augmented interferon and chemokine (CXCL9, CXCL10) response early after virus infection. J Neuroimmunol 193 : 28 37.[CrossRef].[PubMed]
14. Sawyer RA, Selhorst JB, Zimmerman LE, Hoyt WF . 1976. Blindness caused by photoreceptor degeneration as a remote effect of cancer. Am J Ophthalmol 81 : 606 613.[PubMed].[CrossRef]
15. Adamus G, Machnicki M, Elerding H, Sugden B, Blocker YS, Fox DA . 1998. Antibodies to recoverin induce apoptosis of photoreceptor and bipolar cells in vivo. J Autoimmun 11 : 523 533.[CrossRef].[PubMed]
16. Hooks JJ, Tso MO, Detrick B . 2001. Retinopathies associated with antiretinal antibodies. Clin Diagn Lab Immunol 8 : 853 858.[CrossRef].[PubMed]
17. Murphy MA, Thirkill CE, Hart WM Jr . 1997. Paraneoplastic retinopathy: a novel autoantibody reaction associated with small-cell lung carcinoma. J Neuroophthalmol 17 : 77 83.[PubMed]
18. Thirkill CE, FitzGerald P, Sergott RC, Roth AM, Tyler NK, Keltner JL . 1989. Cancer-associated retinopathy (CAR syndrome) with antibodies reacting with retinal, optic-nerve, and cancer cells. N Engl J Med 321 : 1589 1594.[CrossRef].[PubMed]
19. Lei B, Bush RA, Milam AH, Sieving PA . 2000. Human melanoma-associated retinopathy (MAR) antibodies alter the retinal ON-response of the monkey ERG in vivo. Invest Ophthalmol Vis Sci 41 : 262 266.[PubMed]
20. Milam AH, Saari JC, Jacobson SG, Lubinski WP, Feun LG, Alexander KR . 1993. Autoantibodies against retinal bipolar cells in cutaneous melanoma-associated retinopathy. Invest Ophthalmol Vis Sci 34 : 91 100.[PubMed]
21. Dalal MD, Morgans CW, Duvoisin RM, Gamboa EA, Jeffrey BG, Garg SJ, Chan CC, Sen HN . 2013. Diagnosis of occult melanoma using transient receptor potential melastatin 1 (TRPM1) autoantibody testing: a novel approach. Ophthalmology 120 : 2560 2564.[CrossRef].[PubMed]
22. Dhingra A, Fina ME, Neinstein A, Ramsey DJ, Xu Y, Fishman GA, Alexander KR, Qian H, Peachey NS, Gregg RG, Vardi N . 2011. Autoantibodies in melanoma-associated retinopathy target TRPM1 cation channels of retinal ON bipolar cells. J Neurosci 31 : 3962 3967.[CrossRef].[PubMed]
23. Chin MS, Caruso RC, Detrick B, Hooks JJ . 2006. Autoantibodies to p75/LEDGF, a cell survival factor, found in patients with atypical retinal degeneration. J Autoimmun 27 : 17 27.[CrossRef].[PubMed]
24. Comlekoglu DU, Thompson IA, Sen HN . 2013. Autoimmune retinopathy. Curr Opin Ophthalmol 24 : 598 605.[CrossRef].[PubMed]
25. Ferreyra HA, Jayasundera T, Khan NW, He S, Lu Y, Heckenlively JR . 2009. Management of autoimmune retinopathies with immunosuppression. Arch Ophthalmol 127 : 390 397.[CrossRef].[PubMed]
26. Grange L, Dalal M, Nussenblatt RB, Sen HN . 2014. Autoimmune retinopathy. Am J Ophthalmol 157 : 266 272.e1. doi:10.1016/j.ajo.2013.09.019.[CrossRef].[PubMed] http://dx.doi.org/doi:10.1016/j.ajo.2013.09.019
27. Chan CC, Nussenblatt RB, Kim MK, Palestine AG, Awadzi K, Ottesen EA . 1987. Immunopathology of ocular onchocerciasis. 2. Anti-retinal autoantibodies in serum and ocular fluids. Ophthalmology 94 : 439 443.[PubMed].[CrossRef]
28. Braun G, McKechnie NM, Gürr W . 1995. Molecular and immunological characterization of hr44, a human ocular component immunologically cross-reactive with antigen Ov39 of Onchocerca volvulus. J Exp Med 182 : 1121 1131.[PubMed].[CrossRef]
29. McKechnie NM, Gürr W, Braun G . 1997. Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia. J Infect Dis 176 : 1334 1343.[PubMed].[CrossRef]
30. Whittle RM, Wallace GR, Whiston RA, Dumonde DC, Stanford MR . 1998. Human antiretinal antibodies in toxoplasma retinochoroiditis. Br J Ophthalmol 82 : 1017 1021.[PubMed].[CrossRef]
31. Nussenblatt RB, Gery I, Ballintine EJ, Wacker WB . 1980. Cellular immune responsiveness of uveitis patients to retinal S-antigen. Am J Ophthalmol 89 : 173 179.[PubMed].[CrossRef]
32. Morohoshi K, Ohbayashi M, Patel N, Chong V, Bird AC, Ono SJ . 2012. Identification of anti-retinal antibodies in patients with age-related macular degeneration. Exp Mol Pathol 93 : 193 199.[CrossRef].[PubMed]
33. Tan EM, Feltkamp TE, Smolen JS, Butcher B, Dawkins R, Fritzler MJ, Gordon T, Hardin JA, Kalden JR, Lahita RG, Maini RN, McDougal JS, Rothfield NF, Smeenk RJ, Takasaki Y, Wiik A, Wilson MR, Koziol JA . 1997. Range of antinuclear antibodies in “healthy” individuals. Arthritis Rheum 40 : 1601 1611.[CrossRef].[PubMed]
34. Chin MS, Hooper LC, Hooks JJ, Detrick B . 2014. Identification of α-fodrin as an autoantigen in experimental coronavirus retinopathy (ECOR). J Neuroimmunol 272 : 42 50.[CrossRef].[PubMed]
35. Chan CC, Wallace DJ . 2004. Intraocular lymphoma: update on diagnosis and management. Cancer Control 11 : 285 295.[PubMed]
36. Hormigo A, Abrey L, Heinemann MH, DeAngelis LM . 2004. Ocular presentation of primary central nervous system lymphoma: diagnosis and treatment. Br J Haematol 126 : 202 208.[CrossRef].[PubMed]
37. Cassoux N, Merle-Beral H, Lehoang P, Herbort C, Chan CC . 2001. Interleukin-10 and intraocular-central nervous system lymphoma. Ophthalmology 108 : 426 427.[PubMed]
38. Chan CC, Whitcup SM, Solomon D, Nussenblatt RB . 1995. Interleukin-10 in the vitreous of patients with primary intraocular lymphoma. Am J Ophthalmol 120 : 671 673.[PubMed].[CrossRef]
39. Whitcup SM, Stark-Vancs V, Wittes RE, Solomon D, Podgor MJ, Nussenblatt RB, Chan CC . 1997. Association of interleukin 10 in the vitreous and cerebrospinal fluid and primary central nervous system lymphoma. Arch Ophthalmol 115 : 1157 1160.[PubMed].[CrossRef]
40. Wolf LA, Reed GF, Buggage RR, Nussenblatt RB, Chan CC . 2003. Vitreous cytokine levels. Ophthalmology 110 : 1671 1672.[CrossRef].[PubMed]
41. Wang Y, Shen D, Wang VM, Sen HN, Chan CC . 2011. Molecular biomarkers for the diagnosis of primary vitreoretinal lymphoma. Int J Mol Sci 12 : 5684 5697.[CrossRef].[PubMed]


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Antibody responses directed against retinal antigens in sera from patients with retinal diseases

Citation: Hooks J, Chan C, Sen H, Nussenblatt R, Detrick B. 2016. Monitoring Autoimmune Reactivity within the Retina, p 998-1003. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch106

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