Chapter 16 : Introduction

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Introduction, Page 1 of 2

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Since the last publication of the almost a decade ago, developments in computer technology, digital electronics, and laser and fluorochrome chemistry have led to the manufacture, adoption, and common availability of clinical flow cytometers with the capacity to routinely analyze 10 (or more) parameters simultaneously. This “polychromatic flow cytometry” capability requires significant expertise in all phases of clinical flow cytometry, from antibody panel design and sample preparation to cytometer setup and acquisition and, finally, the complex task of analyzing highly parametric data files. The first chapter of this section, from Philip McCoy's laboratory at the National Institutes of Health, Bethesda, MD, provides a comprehensive overview of the technology, sample preparation, quality control, and analytical challenges involved in the design and analysis of polychromatic flow cytometry. This chapter provides insight into the considerations required for the design and analysis of highly parametric flow cytometry assays and alerts the reader to the quality control and quality assurance practices necessary to ensure consistent and reliable polychromatic flow cytometry assays.

Citation: O'Gorman M. 2016. Introduction, p 145-148. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch16
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1. Fletcher M, Sutherland DR, Whitby L, Whitby A, Richards S, Acton A, Keeney M, Borowitz M, Illingworth A, Reilly JT, Barnett D. 22 March 2014. Standardising leucocyte PNH clone detection: an international study. Cytometry B Clin Cytom doi:10.1002/cytob.21174.[CrossRef] http://dx.doi.org/doi:10.1002/cytob.21174
2. Davis BH, Keeney M, Brown R, Illingworth AJ, King MJ, Kumpel B, Meier ER, Sandler SG, Shaz BH, Sutherland DR. 2014. Red Blood Cell Diagnostic Testing Using Flow Cytometry; Approved Guideline, 2nd ed. Document H52-A2. Clinical and Laboratory Standards Institute, Wayne, PA.[CrossRef].[PubMed]
3. Sutherland DR, Acton E, Keeney M, Davis BH, Illingworth A. 2014. Use of CD157 in FLAER-based assays for high-sensitivity PNH neutrophil and PNH monocyte detection. Cytometry B Clin Cytom 86B : 44 55.

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