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Chapter 27 : Cryopreservation of Peripheral Blood Mononuclear Cells
Author:
Adriana Weinberg1
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Affiliations:
1: Department of Pediatrics, Medicine and Pathology, University of Colorado Health Sciences Center, 4200 E. Ninth Ave., Campus Box C 227, Denver, CO 80262
Content Type: Reference
Publication Year:
2016
Category: Immunology
Book DOI:
10.1128/9781555818722
Chapter DOI:
10.1128/9781555818722.ch27
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Cryopreservation of Peripheral Blood Mononuclear Cells, Page 1 of 2
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Abstract:
The utility of cryopreserved peripheral blood mononuclear cells (PBMC) in clinical and diagnostic immunology is widely recognized. The use of cryopreserved PBMC offers multiple advantages for in vitro studies. The ability to batch specimens permits significant cost reductions through efficient utilization of labor and reagents and permits testing of multiple samples in a single run, thus avoiding interassay variability and providing more meaningful comparisons in longitudinal studies.
Citation:
Weinberg A.
2016.
Cryopreservation of Peripheral Blood Mononuclear Cells,
p 263-268.
In
Detrick B, Schmitz J, Hamilton R (ed),
Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555818722.ch27
Figures
Cryopreservation of Peripheral Blood Mononuclear Cells
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Citation:
Weinberg A.
2016.
Cryopreservation of Peripheral Blood Mononuclear Cells,
p 263-268.
In
Detrick B, Schmitz J, Hamilton R (ed),
Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555818722.ch27
/content/10.1128/9781555818722.ch27.ch27fig1
FIGURE 1
CMV-specific RCF in cryopreserved versus fresh PBMC from HIV-infected patients and uninfected controls. Data were derived from samples from HIV-infected patients (HIV+, triangles) and uninfected controls (HIV−, squares). The diagonal represents the slope of equivalence between fresh- and frozen-cell assays.
10.1128/9781555818722/8722ch27fig1_thmb.gif
10.1128/9781555818722/8722ch27fig1.gif
FIGURE 1
CMV-specific RCF in cryopreserved versus fresh PBMC from HIV-infected patients and uninfected controls. Data were derived from samples from HIV-infected patients (HIV+, triangles) and uninfected controls (HIV−, squares). The diagonal represents the slope of equivalence between fresh- and frozen-cell assays.
Citation:
Weinberg A.
2016.
Cryopreservation of Peripheral Blood Mononuclear Cells,
p 263-268.
In
Detrick B, Schmitz J, Hamilton R (ed),
Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555818722.ch27
/content/10.1128/9781555818722.ch27.ch27fig2
FIGURE 2
Effect of viability on cryopreserved PBMC LPA results. Data were derived from samples from HIV-infected patients. The panels on the left show that when analyzing all samples, the LPA results significantly increase with higher viability. A breakpoint in the distribution pattern can be observed at a viability of 70%. The panels on the right show that when analyzing only samples with a viability of ≥70%, the LPA results are independent of the PBMC viability. SI, stimulation index.
10.1128/9781555818722/8722ch27fig2_thmb.gif
10.1128/9781555818722/8722ch27fig2.gif
FIGURE 2
Effect of viability on cryopreserved PBMC LPA results. Data were derived from samples from HIV-infected patients. The panels on the left show that when analyzing all samples, the LPA results significantly increase with higher viability. A breakpoint in the distribution pattern can be observed at a viability of 70%. The panels on the right show that when analyzing only samples with a viability of ≥70%, the LPA results are independent of the PBMC viability. SI, stimulation index.
Citation:
Weinberg A.
2016.
Cryopreservation of Peripheral Blood Mononuclear Cells,
p 263-268.
In
Detrick B, Schmitz J, Hamilton R (ed),
Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555818722.ch27
/content/10.1128/9781555818722.ch27.ch27fig3
FIGURE 3
CMV-specific IFN-γ in cryopreserved versus fresh PBMC from HIV-infected patients (triangles) and controls (squares). Data were derived from samples from HIV-infected patients and uninfected controls. The diagonal represents the slope of equivalence between fresh- and frozen-cell assays.
10.1128/9781555818722/8722ch27fig3_thmb.gif
10.1128/9781555818722/8722ch27fig3.gif
FIGURE 3
CMV-specific IFN-γ in cryopreserved versus fresh PBMC from HIV-infected patients (triangles) and controls (squares). Data were derived from samples from HIV-infected patients and uninfected controls. The diagonal represents the slope of equivalence between fresh- and frozen-cell assays.
Citation:
Weinberg A.
2016.
Cryopreservation of Peripheral Blood Mononuclear Cells,
p 263-268.
In
Detrick B, Schmitz J, Hamilton R (ed),
Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555818722.ch27
/content/10.1128/9781555818722.ch27.ch27fig4
FIGURE 4
Effect of cryopreservation on flow cytometric immunophenotyping. Data were derived from samples from HIV-infected subjects (triangles) and controls (squares). The diagonal represents the slope of equivalence between fresh- and frozen-cell assays. (A) Example of a cell surface marker that is well preserved during cryopreservation (CD95). (B) Loss of CD62L during cryopreservation.
10.1128/9781555818722/8722ch27fig4_thmb.gif
10.1128/9781555818722/8722ch27fig4.gif
FIGURE 4
Effect of cryopreservation on flow cytometric immunophenotyping. Data were derived from samples from HIV-infected subjects (triangles) and controls (squares). The diagonal represents the slope of equivalence between fresh- and frozen-cell assays. (A) Example of a cell surface marker that is well preserved during cryopreservation (CD95). (B) Loss of CD62L during cryopreservation.
Citation:
Weinberg A.
2016.
Cryopreservation of Peripheral Blood Mononuclear Cells,
p 263-268.
In
Detrick B, Schmitz J, Hamilton R (ed),
Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition.
ASM Press, Washington, DC.
doi: 10.1128/9781555818722.ch27
References
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