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Category: Immunology
Functional Assays for B Cells and Antibodies, Page 1 of 2
< Previous page | Next page > /docserver/preview/fulltext/10.1128/9781555818722/9781555818715_CH29-1.gif /docserver/preview/fulltext/10.1128/9781555818722/9781555818715_CH29-2.gifAbstract:
The primary cells of the adaptive immune system are T cells, B cells, and natural killer cells. These lymphocytes assist the host in eliminating both intracellular pathogens (T cells and NK cells) and extracellular pathogens (B cells) through B cell-T cell interactions, as well as interactions with other cells and molecules of the innate immune system. B cells recognize foreign antigen by the B-cell receptor (BCR), a membrane-bound immunoglobulin generated through a complex genetic recombination process (1). The BCR recognizes conformational protein antigens as well as nonprotein antigens. Two types of B cells have been described based on expression of cell surface molecules and function. B1 (CD5+) B cells are thought to be a more “natural” type of B cell which respond to T-cell-independent forms of antigen (i.e., bacterial polysaccharides) (2, 3). B2 B cells respond to T-cell-dependent antigens, such as the classic protein antigens tetanus and diphtheria toxoids. Both classes of B cells respond to BCR binding of antigen by proliferation, differentiation into antibody-secreting plasma cells, and formation of memory B cells.
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Assay plate layout.
Assay plate layout.
B-cell markers used in flow cytometry
B-cell markers used in flow cytometry
Associations with humoral or B-lymphocyte deficiencies
Associations with humoral or B-lymphocyte deficiencies
In vitro activators of B cell proliferation and immunoglobulin production
In vitro activators of B cell proliferation and immunoglobulin production
Pneumococcal target strains for the MOPA a