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Chapter 29 : Functional Assays for B Cells and Antibodies

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Abstract:

The primary cells of the adaptive immune system are T cells, B cells, and natural killer cells. These lymphocytes assist the host in eliminating both intracellular pathogens (T cells and NK cells) and extracellular pathogens (B cells) through B cell-T cell interactions, as well as interactions with other cells and molecules of the innate immune system. B cells recognize foreign antigen by the B-cell receptor (BCR), a membrane-bound immunoglobulin generated through a complex genetic recombination process (1). The BCR recognizes conformational protein antigens as well as nonprotein antigens. Two types of B cells have been described based on expression of cell surface molecules and function. B1 (CD5) B cells are thought to be a more “natural” type of B cell which respond to T-cell-independent forms of antigen (i.e., bacterial polysaccharides) (2, 3). B2 B cells respond to T-cell-dependent antigens, such as the classic protein antigens tetanus and diphtheria toxoids. Both classes of B cells respond to BCR binding of antigen by proliferation, differentiation into antibody-secreting plasma cells, and formation of memory B cells.

Citation: Nahm M, Burton R. 2016. Functional Assays for B Cells and Antibodies, p 280-289. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch29
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FIGURE 1

Assay plate layout.

Citation: Nahm M, Burton R. 2016. Functional Assays for B Cells and Antibodies, p 280-289. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch29
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Tables

Generic image for table
TABLE 1

B-cell markers used in flow cytometry

Citation: Nahm M, Burton R. 2016. Functional Assays for B Cells and Antibodies, p 280-289. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch29
Generic image for table
TABLE 2

Associations with humoral or B-lymphocyte deficiencies

Citation: Nahm M, Burton R. 2016. Functional Assays for B Cells and Antibodies, p 280-289. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch29
Generic image for table
TABLE 3

activators of B cell proliferation and immunoglobulin production

Citation: Nahm M, Burton R. 2016. Functional Assays for B Cells and Antibodies, p 280-289. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch29
Generic image for table
TABLE 4

Pneumococcal target strains for the MOPA

Citation: Nahm M, Burton R. 2016. Functional Assays for B Cells and Antibodies, p 280-289. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch29

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