Chapter 6 : Immunoglobulin Genes

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Immunoglobulin Genes, Page 1 of 2

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Immunoglobulins are a heterogeneous group of glycoproteins produced by B lymphocytes and plasma cells. A single person can synthesize 10 million to 100 million different immunoglobulin molecules, each having distinct antigen-binding specificities. This great diversity in the so-called humoral immune system allows us to generate antibodies specific for a variety of substances, including synthetic molecules not naturally present in our environment. Despite the diversity in the specificities of antibody molecules, the binding of an antibody to an antigen initiates a limited series of biologically important effector functions, such as complement activation and/or adherence of the immune complex to receptors on leukocytes (1). Resolution of the immunoglobulin structure has revealed how these molecules can have such great diversity in antigen-binding activities while maintaining conserved effector functions, such as complement activation.

Citation: Kipps T, Ghia E, Rassenti L. 2016. Immunoglobulin Genes, p 51-64. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch6
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Image of FIGURE 1

Immunoglobulin heavy-chain gene complex. The heavy-chain genes encoding the constant regions are represented by blue boxes. Switch regions are represented by a filled circle upstream of the IGHC genes. Enhancers are represented by light blue circles. Each IGHV, IGHD, and IGHJ gene is labeled on the right of each symbol. Functional IGHV, IGHD, and IGHJ genes are represented by green boxes, blue lines, and yellow lines, respectively. IGHV, IGJH, and IGHC pseudogenes are represented by red boxes, orange boxes, and blue open boxes, respectively. IGHV and IGHC open reading frames are represented by yellow boxes and blue dashed boxes, respectively. Unrelated pseudogenes are represented by purple open boxes. Colors are according to the international ImMunoGeneTics information system (IMGT) color menu for genes. Reproduced with the kind authorization of Marie-Paule Lefranc (IMGT [http://www.imgt.org]).

Citation: Kipps T, Ghia E, Rassenti L. 2016. Immunoglobulin Genes, p 51-64. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch6
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Image of FIGURE 2

Immunoglobulin light-chain gene complexes. (Left) κ light-chain gene complex on chromosome 2p11-12. The blue box represents the functional IGLC gene. The IGKJ gene segments are indicated by yellow lines labeled “J1 to 5.” The κ light-chain enhancers are represented by light blue circles. IGKV functional genes, pseudogenes, and open reading frame are indicated by green, red, and yellow boxes, respectively. The IGKV genes of the p region are designated by a number for the subgroup, followed by a hyphen and a number for the localization from 3′ to 5′ in the locus. The IGKV genes of the d region are designated by the same numbers as the corresponding genes in the p region, with the letter D added. Arrows show the IGKV genes whose orientation is opposite to that of the IGKJ gene segments. (Right) λ light-chain gene complex on chromosome 22q11.2. The blue boxes represent functional IGLJ and IGLC gene segments, whereas blue open boxes represent IGLJ and IGLC pseudogenes. IGLV functional genes, pseudogenes, and open reading frame are indicated by green, red, and yellow boxes, respectively. IGLV pseudogenes that could not be assigned to subgroups with functional genes are represented by red boxes and designated by a roman number in parentheses, corresponding to the clans, followed by a dash and a number for the localization from 3′ to 5′ in the locus. The IGLV genes are organized into three clusters, designated A, B, and C, which are indicated to the left of each cluster. Unrelated pseudogenes are represented by purple open boxes. The λ light-chain enhancer is represented by a light blue circle. Colors are according to the IMGT color menu for genes. Reproduced with the kind authorization of Marie-Paule Lefranc (IMGT, the international ImMunoGeneTics information system [http://www.imgt.org]).

Citation: Kipps T, Ghia E, Rassenti L. 2016. Immunoglobulin Genes, p 51-64. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch6
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Physical properties of human immunoglobulins

Citation: Kipps T, Ghia E, Rassenti L. 2016. Immunoglobulin Genes, p 51-64. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch6
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Sense and antisense strand oligonucleotide primers to amplify the rearranged V gene in genomic DNA or cDNA

Citation: Kipps T, Ghia E, Rassenti L. 2016. Immunoglobulin Genes, p 51-64. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch6

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