Chapter 67 : Arboviruses

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The arthropod-borne viruses (arboviruses) represent a diverse amalgam of more than 500 animal viruses that are grouped together because of their ability to replicate in both vertebrates and arthropods. While more than 150 arboviruses are known to cause human illness or infection, the list of the most medically important viruses is considerably shorter (Table 1) (1–3). Because of the vast number of agents that potentially should be considered in a differential diagnosis, the use of epidemiologic, ecologic, and clinical data to guide the choice and interpretation of clinical laboratory diagnostic tests is essential. Most arboviruses are transmitted seasonally in specific geographic locations or ecological habitats. The patient's history of travel, activities, and potential exposures to arthropods or habitats associated with arbovirus transmission provides vital data for selection of appropriate antigens and relevant diagnostic approaches. Knowledge of the patient's immunizations against yellow fever (YF), Japanese encephalitis (JE), or tick-borne encephalitis (TBE) viruses is also important for the proper interpretation of serological results. Although the laboratory diagnosis of arboviral infections still relies chiefly on serology, other approaches that directly detect viral antigen or genomic material and not antibodies are now routine.

Citation: Lanciotti R, Roehrig J. 2016. Arboviruses, p 648-657. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch67
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1. Karabatsos N . 1985. International Catalogue of Arboviruses. American Society for Tropical Medicine and Hygiene, San Antonio, TX.
2. Monath TP . 1988. Arboviruses: Epidemiology and Ecology, vol 1. CRC Press, Boca Raton, FL.
3. Tsai TF, . 1995. Arboviruses, p 980 996. In Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (ed), Manual of Clinical Microbiology, 6th ed. American Society for Microbiology, Washington, DC.
4. Burke DS, Nisalak A, Ussery MA, Laorakpongse T, Chantavibul S . 1985. Kinetics of IgM and IgG responses to Japanese encephalitis virus in human serum and cerebrospinal fluid. J Infect Dis 151 : 1093 1099.[PubMed].[CrossRef]
5. Roehrig JT, Brown TM, Johnson AJ, Karabatsos N, Martin DM, Mitchell CJ, Nasci R, . 1998. Alphaviruses, p 7 18. In Stephenson JR, Warnes A (ed), Methods in Molecular Biology: Diagnostic Virology Protocols. Humana Press, Totowa, NJ.
6. Wong SJ, Boyle RH, Demarest VL, Woodmansee AN, Kramer LD, Li H, Drebot M, Koski RA, Fikrig E, Martin DA, Shi PY . 2003. Immunoassay targeting nonstructural protein 5 to differentiate West Nile virus infection from dengue and St. Louis encephalitis virus infections and from flavivirus vaccination. J Clin Microbiol 41 : 4217 4223.[PubMed].[CrossRef]
7. Wong SJ, Demarest VL, Boyle RH, Wang T, Ledizet M, Kar K, Kramer LD, Fikrig E, Koski RA . 2004. Detection of human anti-flavivirus antibodies with a west nile virus recombinant antigen microsphere immunoassay. J Clin Microbiol 42 : 65 72.[CrossRef].[PubMed]
8. Basile AJ, Horiuchi K, Panella AJ, Laven J, Kosoy O, Lanciotti RS, Venkateswaran N, Biggerstaff BJ . 2013. Multiplex microsphere immunoassays for the detection of IgM and IgG to arboviral diseases. PLoS One 8( 9) : e75670. doi:10.1371/journal.pone.0075670[CrossRef].[PubMed] http://dx.doi.org/doi:10.1371/journal.pone.0075670
9. Johnson AJ, Noga AJ, Kosoy O, Lanciotti RS, Johnson AA, Biggerstaff BJ . 2005. Duplex microsphere-based immunoassay for detection of anti-West Nile virus and anti-St. Louis encephalitis virus immunoglobulin m antibodies. Clin Diagn Lab Immunol 12 : 566 574.[CrossRef].[PubMed]
10. Johnson AJ, Martin DA, Karabatsos N, Roehrig JT . 2000. Detection of anti-arboviral immunoglobulin G by using a monoclonal antibody-based capture enzyme-linked immunosorbent assay. J Clin Microbiol 38 : 1827 1831.[PubMed]
11. Innis BL, Nisalak A, Nimmannitya S, Kusalerdchariya S, Chongswasdi V, Suntayakorn S, Puttisri P, Hoke CH . 1989. An enzyme-linked immunosorbent assay to characterize dengue infections where dengue and Japanese encephalitis co-circulate. Am J Trop Med Hyg 40 : 418 427.[PubMed]
12. Thibodeaux BA, Liss NM, Panella AN, Roehrig JT . 2011. Development of a human-murine chimeric immunoglobulin M for use in the serological detection of human alphavirus antibodies. Clin Vaccine Immunol 18 : 2181 2182.[CrossRef].[PubMed]
13. Thibodeaux BA, Panella AN, Roehrig JT . 2010. Development of human-murine chimeric immunoglobulin G for use in the serological detection of human flavivirus and alphavirus antibodies. Clin Vaccine Immunol 17 : 1617 1623.[CrossRef].[PubMed]
14. Thibodeaux BA, Roehrig JT . 2009. Development of a human-murine chimeric immunoglobulin M antibody for use in the serological detection of human flavivirus antibodies. Clin Vaccine Immunol 16 : 679 685.[CrossRef].[PubMed]
15. Davis BS, Chang GJ, Cropp B, Roehrig JT, Martin DA, Mitchell CJ, Bowen R, Bunning ML . 2001. West Nile virus recombinant DNA vaccine protects mouse and horse from virus challenge and expresses in vitro a noninfectious recombinant antigen that can be used in enzyme-linked immunosorbent assays. J Virol 75 : 4040 4047.[CrossRef].[PubMed]
16. Chiou SS, Fan YC, Crill WD, Chang RY, Chang GJ . 2012. Mutation analysis of the cross-reactive epitopes of Japanese encephalitis virus envelope glycoprotein. J Gen Virol 93( Pt 6) : 1185 1192.[CrossRef].[PubMed]
17. Beaty BJ, Calisher CH, Shope RW, . 1989. Arboviruses, p 797 856. In Schmidt NJ, Emmons RW (ed), Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 6th ed. American Public Health Association, Washington, DC.
18. Roehrig JT, Hombach J, Barrett AD . 2008. Guidelines for plaque-reduction neutralization testing of human antibodies to dengue viruses. Viral Immunol 21 : 123 132.[CrossRef].[PubMed]
19. Johnson BW, Kosoy O, Wang E, Delorey M, Russell B, Bowen RA, Weaver SC . 2011. Use of sindbis/eastern equine encephalitis chimeric viruses in plaque reduction neutralization tests for arboviral disease diagnostics. Clin Vaccine Immunol 18 : 1486 1491.[CrossRef].[PubMed]
20. Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, Matheus S, Baril L . 2008. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. PLoS Negl Trop Dis 2( 8) : e280. doi:10.1371/journal.pntd.0000280[CrossRef].[PubMed] http://dx.doi.org/doi:10.1371/journal.pntd.0000280
21. Tsai TF, Bolin RA, Montoya M, Bailey RE, Francy DB, Jozan M, Roehrig JT . 1987. Detection of St. Louis encephalitis virus antigen in mosquitoes by capture enzyme immunoassay. J Clin Microbiol 25 : 370 376.[PubMed]
22. De Brito T, Siqueira SA, Santos RT, Nassar ES, Coimbra TL, Alves VA . 1992. Human fatal yellow fever. Immunohistochemical detection of viral antigens in the liver, kidney and heart. Pathol Res Pract 188 : 177 181.[CrossRef].[PubMed]
23. Hunt AR, Roehrig JT . 1985. Biochemical and biological characteristics of epitopes on the E1 glycoprotein of western equine encephalitis virus. Virology 142 : 334 346.[PubMed].[CrossRef]
24. Roehrig JT, . 1986. The use of monoclonal antibodies in studies of the structural proteins of alphaviruses and flaviviruses, p 251 278. In Schlesinger S, Schlesinger MJ (ed), The Viruses: The Togaviridae and Flaviviridae. Plenum Press, New York, NY.
25. Roehrig JT, Bolin RA . 1997. Monoclonal antibodies capable of distinguishing epizootic from enzootic varieties of subtype 1 Venezuelan equine encephalitis viruses in a rapid indirect immunofluorescence assay. J Clin Microbiol 35 : 1887 1890.[PubMed]
26. Roehrig JT, Hunt AR, Chang GJ, Sheik B, Bolin RA, Tsai TF, Trent DW . 1990. Identification of monoclonal antibodies capable of differentiating antigenic varieties of eastern equine encephalitis viruses. Am J Trop Med Hyg 42 : 394 398.[PubMed]
27. Roehrig JT, Mathews JH, Trent DW . 1983. Identification of epitopes on the E glycoprotein of Saint Louis encephalitis virus using monoclonal antibodies. Virology 128 : 118 126.[PubMed].[CrossRef]
28. Monath TP, Ballinger ME, Miller BR, Salaun JJ . 1989. Detection of yellow fever viral RNA by nucleic acid hybridization and viral antigen by immunocytochemistry in fixed human liver. Am J Trop Med Hyg 40 : 663 668.[PubMed]
29. Lanciotti RS, Kosoy OI, Bosco-Lauth AM, Pohl J, Stuchlik O, Reed M, Lambert AJ . 2013. Isolation of a novel orthobunyavirus (Brazoran virus) with a 1.7 kb S segment that encodes a unique nucleocapsid protein possessing two putative functional domains. Virology 444 : 55 63.[CrossRef].[PubMed]
30. Lanciotti RS, Kerst AJ, Nasci RS, Godsey MS, Mitchell CJ, Savage HM, Komar N, Panella NA, Allen BC, Volpe KE, Davis BS, Roehrig JT . 2000. Rapid detection of west nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay. J Clin Microbiol 38 : 4066 4071.[PubMed]
31. Shi PY, Kauffman EB, Ren P, Felton A, Tai JH, Dupuis AP II, Jones SA, Ngo KA, Nicholas DC, Maffei J, Ebel GD, Bernard KA, Kramer LD . 2001. High-throughput detection of West Nile virus RNA. J Clin Microbiol 39 : 1264 1271.[CrossRef].[PubMed]
32. Kuno G . 1998. Universal diagnostic RT-PCR protocol for arboviruses. J Virol Methods 72 : 27 41.[PubMed].[CrossRef]
33. Kuno G, Mitchell CJ, Chang GJ, Smith GC . 1996. Detecting bunyaviruses of the Bunyamwera and California serogroups by a PCR technique. J Clin Microbiol 34 : 1184 1188.[PubMed]
34. Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV . 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30 : 545 551.[PubMed]
35. Pfeffer M, Proebster B, Kinney RM, Kaaden OR . 1997. Genus-specific detection of alphaviruses by a semi-nested reverse transcription-polymerase chain reaction. Am J Trop Med Hyg 57 : 709 718.[PubMed]
36. Scaramozzino N, Crance J-M, Jouan A, DeBriel DA, Stoll F, Garin D . 2001. Comparison of flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse transcription-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequences. J Clin Microbiol 39 : 1922 1927.[CrossRef].[PubMed]
37. Lambert AJ, Martin DA, Lanciotti RS . 2003. Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays. J Clin Microbiol 41 : 379 385.[PubMed].[CrossRef]
38. Lanciotti RS, Kosoy OL, Laven JJ, Panella AJ, Velez JO, Lambert AJ, Campbell GL . 2007. Chikungunya virus in US travelers returning from India, 2006. Emerg Infect Dis 13 : 764 767.[CrossRef].[PubMed]
39. Laue T, Emmerich P, Schmitz H . 1999. Detection of dengue virus RNA in patients after primary or secondary dengue infection by using the TaqMan automated amplification system. J Clin Microbiol 37 : 2543 2547.[PubMed]
40. Wicki R, Sauter P, Mettler C, Natsch A, Enzler T, Pusterla N, Kuhnert P, Egli G, Bernasconi M, Lienhard R, Lutz H, Leutenegger CM . 2000. Swiss Army Survey in Switzerland to determine the prevalence of Francisella tularensis, members of the Ehrlichia phagocytophila genogroup, Borrelia burgdorferi sensu lato, and tick-borne encephalitis virus in ticks. Eur J Clin Microbiol Infect Dis 19 : 427 432.[PubMed].[CrossRef]
41. Wu SJ, Lee EM, Putvatana R, Shurtliff RN, Porter KR, Suharyono W, Watts DM, King CC, Murphy GS, Hayes CG, Romano JW . 2001. Detection of dengue viral RNA using a nucleic acid sequence-based amplification assay. J Clin Microbiol 39 : 2794 2798.[CrossRef].[PubMed]
42. Lanciotti RS, Kerst AJ . 2001. Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J Clin Microbiol 39 : 4506 4513.[CrossRef].[PubMed]
43. Mori Y, Kanda H, Notomi T . 2013. Loop-mediated isothermal amplification (LAMP): recent progress in research and development. J Infect Chemother 19 : 404 411.[CrossRef].[PubMed]
44. Hayasaka D, Aoki K, Morita K . 2013. Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification. Virol J 10 : 68.[CrossRef].[PubMed]
45. Kwallah AO, Inoue S, Muigai AW, Kubo T, Sang R, Morita K, Mwau M . 2013. A real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus. J Virol Methods 193 : 23 27.[CrossRef].[PubMed]
46. Le Roux CA, Kubo T, Grobbelaar AA, van Vuren PJ, Weyer J, Nel LH, Swanepoel R, Morita K, Paweska JT . 2009. Development and evaluation of a real-time reverse transcription-loop-mediated isothermal amplification assay for rapid detection of Rift Valley fever virus in clinical specimens. J Clin Microbiol 47 : 645 651.[CrossRef].[PubMed]
47. Li S, Fang M, Zhou B, Ni H, Shen Q, Zhang H, Han Y, Yin J, Chang W, Xu G, Cao G . 2011. Simultaneous detection and differentiation of dengue virus serotypes 1-4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay. Virol J 8 : 360.[CrossRef].[PubMed]
48. Osman HA, Eltom KH, Musa NO, Bilal NM, Elbashir MI, Aradaib IE . 2013. Development and evaluation of loop-mediated isothermal amplification assay for detection of Crimean Congo hemorrhagic fever virus in Sudan. J Virol Methods 190 : 4 10.[CrossRef].[PubMed]
49. Parida MM, Santhosh SR, Dash PK, Tripathi NK, Lakshmi V, Mamidi N, Shrivastva A, Gupta N, Saxena P, Babu JP, Rao PV, Morita K . 2007. Rapid and real-time detection of Chikungunya virus by reverse transcription loop-mediated isothermal amplification assay. J Clin Microbiol 45 : 351 357.[CrossRef].[PubMed]
50. Teoh BT, Sam SS, Tan KK, Johari J, Danlami MB, Hooi PS, Md-Esa R, AbuBakar S . 2013. Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification. BMC Infect Dis 13 : 387.[CrossRef].[PubMed]


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Characteristics of selected medically important arbovirus disease

Citation: Lanciotti R, Roehrig J. 2016. Arboviruses, p 648-657. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch67
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Selected arbovirus monoclonal antibodies for serologic and antigen detection assays

Citation: Lanciotti R, Roehrig J. 2016. Arboviruses, p 648-657. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch67

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