Chapter 8 : Animal Models of Enterohemorrhagic Infection

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Since the first recognized O157:H7 outbreak over 3 decades ago ( ), investigators have sought to identify suitable animal hosts that allow study of enterohemorrhagic (EHEC)-mediated disease. The value of any animal infection model ultimately relies on its ability to reproduce the human disease and enable the mechanistic processes that lead to clinical disease, pathogen carriage, and transmission to be examined. As yet, no single animal model mimics the full spectrum of disease caused by EHEC infection. However, since Moxley and Francis's review in 1998 ( ), several advances have been made in the field, including the generation of a Shiga toxin (Stx)-producing -murine model, a human intestine xenograft murine model, and a renewed interest in the use of rabbit models. This article reviews what is known about EHEC-mediated disease from human outbreaks and biopsy studies, and within a historical context, describes the features and limitations of EHEC infection models that are based on the three most commonly used species (pigs, rabbits, and mice). Recent new advances are highlighted and discussed in light of mounting evidence for the need to study the biology and virulence strategies of EHEC in the context of its niche within the intestine. The reader is directed elsewhere for excellent reviews on the environmental sources of EHEC infection ( ), EHEC interactions with the intestinal epithelium ( ), the molecular basis of pathogenicity ( ), and the current status of treatment options ( ).

Citation: Ritchie J. 2015. Animal Models of Enterohemorrhagic Infection, p 175-195. In Sperandio V, Hovde C (ed), Enterohemorrhagic and Other Shiga Toxin-Producing . ASM Press, Washington, DC. doi: 10.1128/microbiolspec.EHEC-0022-2013
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Comparative murine models of EHEC-mediated disease

Citation: Ritchie J. 2015. Animal Models of Enterohemorrhagic Infection, p 175-195. In Sperandio V, Hovde C (ed), Enterohemorrhagic and Other Shiga Toxin-Producing . ASM Press, Washington, DC. doi: 10.1128/microbiolspec.EHEC-0022-2013
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Comparative models of EHEC-mediated disease in species other than pigs, rabbits, and mice

Citation: Ritchie J. 2015. Animal Models of Enterohemorrhagic Infection, p 175-195. In Sperandio V, Hovde C (ed), Enterohemorrhagic and Other Shiga Toxin-Producing . ASM Press, Washington, DC. doi: 10.1128/microbiolspec.EHEC-0022-2013

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