1887

Chapter 10.2 : Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures

Editor: Amy L. Leber1
VIEW AFFILIATIONS HIDE AFFILIATIONS
Affiliations: 1: Editor in chief, Department of Laboratory Medicine, Nationwide Children’s Hospital, Columbus, Ohio
Content Type: Reference
Publication Year: 2016

Category: Clinical Microbiology

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Preview this chapter:
Preview Magnify
Zoom in
Zoomout

Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap10.2-1.gif /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap10.2-2.gif

Abstract:

Viruses may contain genetic information for many functions in addition to viral structural protein genes, notably those associated with replication and immune system modulation. However, even viruses with very large genomes and extensive viral-coded functions are all obligate intracellular parasites, requiring metabolically active cells to support their replication. Many human pathogenic viruses can be cultured in readily available cell culture monolayers ( Table 10.2–1 ). However, some can be isolated only by using specialized systems ( Table 10.2–2 ) such as organ culture, suspension leukocyte culture, or animals. Additionally, there are several agents for which no system has been identified.

Citation: Leber A. 2016. Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures, p 10.2.1-10.2.11. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch10.2
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

Figures

Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures
Citation: Leber A. 2016. Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures, p 10.2.1-10.2.11. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch10.2
/content/10.1128/9781555818814.chap10.2.fig10.2-1
Figure 10.2–1
Assessment, incubation, and maintenance of uninoculated cell cultures.
10.1128/9781555818814/fig10.2-1_thmb.gif
10.1128/9781555818814/fig10.2-1.gif
Image of Figure 10.2–1
Figure 10.2–1

Assessment, incubation, and maintenance of uninoculated cell cultures.

Citation: Leber A. 2016. Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures, p 10.2.1-10.2.11. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch10.2
Permissions and Reprints Request Permissions
Download as Powerpoint
/content/10.1128/9781555818814.chap10.2.fig10.2-2
Figure 10.2–2
Focal area of replicating A-549 cells that have cross-contaminated an MRC-5 monolayer.
10.1128/9781555818814/fig10.2-2_thmb.gif
10.1128/9781555818814/fig10.2-2.gif
Image of Figure 10.2–2
Figure 10.2–2

Focal area of replicating A-549 cells that have cross-contaminated an MRC-5 monolayer.

Citation: Leber A. 2016. Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures, p 10.2.1-10.2.11. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch10.2
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555818814.chap10.2
1. Clarke L. 2010. Selection, maintenance and observation of uninoculated monolayer cell cultures. In Garcia LS (ed), Clinical Microbiology Procedures Handbook, 3rd ed. ASM Press, Washington, DC.
2. Gleaves CA, Smith TF, Shuster EA, Pearson GR. 1984. Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J Clin Microbiol 19: 917– 919.
3. Paya CV, Wold AD, Smith TF. 1987. Detection of cytomegalovirus infections in specimens other than urine by the shell vial assay and conventional tube cell cultures. J Clin Microbiol 25: 755– 757.
4. Gleaves CA, Lee CF, Bustamante CI, Meyers JD. 1988. Use of murine monoclonal antibodies for laboratory diagnosis of varicellazoster infection. J Clin Microbiol 26: 1623– 1625.
5. Schirm J, Janneke JM, Pastoor GW, vanVoorst Vader PC, Schroder FP. 1989. Rapid detection of varicella-zoster virus in clinical specimens using monoclonal antibodies on shell vials and smears. J Med Virol 28: 1– 6.
6. West PG, Aldrich B, Hartwig R, Haller GJ. 1988. Increased detection rate for varicellazoster virus with combination of two techniques. J Clin Microbiol 26: 2680– 2681.
7. Gleaves CA, Wilson DJ, Wold AD, Smith TF. 1985. Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation. J Clin Microbiol 21: 29– 32.
8. Espy MJ, Hierholzer C, Smith TF. 1987. The effect of centrifugation on the rapid detection of adenovirus in shell vials. J Clin Pathol 88: 358– 360.
9. Espy MJ, Smith TF, Haromon MW, Kendal AP. 1986. Rapid detection of influenza virus by shell vial assay with monoclonal antibodies. J Clin Microbiol 24: 677– 679.
10. Frank AL, Couch RB, Griffis CA, Baxter BD. 1979. Comparison of different tissue cultures for isolation and quantitation of influenza and parainfluenza viruses. J Clin Microbiol 10: 32– 36.
11. Johnston SLG, Siegel CS. 1990. Evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus. J Clin Microbiol 28: 2394– 2397.
12. Matthey S, Nicholson D, Ruhs S, Alden B, Knock M, Schultz K, Schmuecker A. 1992. Rapid detection of respiratory viruses by shell vial culture and direct staining by using pooled and individual monoclonal antibodies. J Clin Microbiol 30: 540– 544.
13. Olsen MA, Shuck KM, Sambol AR, Flor SM, O’Brien J, Cabrera BJ. 1993. Isolation of seven respiratory viruses in shell vials: a practical and highly sensitive method. J Clin Microbiol 31: 422– 425.
14. Smith MC, Creutz C, Huang YT. 1991. Detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique. J Clin Microbiol 29: 463– 465.
15. Waris M, Aiegler T, Kivivirta M, Ruuskananen O. 1990. Rapid detection of respiratory syncytial virus and influenza A virus in cell cultures by immunoperoxidase staining with monoclonal antibodies. J Clin Microbiol 28: 1159– 1162.
16. Minnich LL, Goodenough F, Ray CG. 1991. Use of immunofluorescence to identify measles virus infections. J Clin Microbiol 29: 1148– 1150.
17. Landry ML, Ferguson D. 1993. Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease. J Clin Microbiol 31: 2851– 2856.
18. Proffitt MR, Schindler SA. 1995. Rapid detection of HSV with an enzyme-linked virus inducible system (ELVIS) employing a genetically modified cell line. Clin Diagn Virol 4: 175– 182.
19. Buck GE, Wiesemann M, Stewart L. 2002. Comparison of mixed cell culture containing genetically engineered BGMK and CaCo-2 cells (Super E-Mix) with RT-PCR and conventional cell culture for the diagnosis of enterovirus meningitis. J Clin Virol 25( Suppl 1): S13– S18.
20. Ono N, Tatsuo H, Hidaka Y, Aoki T, Minagawa H, Yanagi Y. 2001. Measles viruses on throat swabs from measles patients use signaling lymphocytic activation molecule (CDw150) but not CD46 as a cellular receptor. J Virol 75: 4399- 4401.
21. Takeuchi K, Miyajima N, Nagata N, Takeda M, Tashiro M. 2003. Wild-type measles virus induces large syncytium formation in primary human airway epithelial cells by a SLAM(CD150)-independent mechanism. Virus Res 94: 11– 16.
22. Oh DY, Barr IG, Mosse JA, Laurie KL. 2008. MDCK-SIAT1 cells show improved isolation rates for recent human influenza viruses compared to conventional MDCK cells. J Clin Microbiol 46: 2189– 2194.
23. Albert MJ. 1986. Enteric adenoviruses. Arch Virol 88: 1– 17.
24. Takiff HE, Straus SE, Garon CF. 1981. Propagation and in vitro studies of previously noncultivatable enteral adenoviruses in 293 cells. Lancet ii: 832– 834.
25. Fouchier RA, Hartwig NG, Bestebroer TM, Niemeyer B, de Jong JC, Simon JH, Osterhaus AD. 2004. A previously undescribed coronavirus associated with respiratory disease in humans. Proc Natl Acad Sci USA 101: 6212– 6216.
26. Chan PKS, Tam JS, Lam CW, Chan E, Wu A, Li CK, Buckley TA, Ng KC, Joynt GM, Cheng FW, To KF, Lee N, Hui DS, Cheung JL, Chu I, Liu E, Chung SS, Sung JJ. 2003. Human metapneumovirus detection in patients with severe acute respiratory syndrome. Emerg Infect Dis 9: 1058– 1063.
27. Gillim-Ross L, Taylor J, Scholl DR, Ridenour J, Masters PS, Wentworth DE. 2004. Discovery of novel human and animal cells infected by the severe acute respiratory syndrome coronavirus by replication-specific multiplex reverse transcription-PCR. J Clin Microbiol 42: 3196– 3206.
28. Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, Tong S, Urbani C, Comer JA, Lim W, Rollin PE, Dowell SF, Ling AE, Humphrey CD, Shieh WJ, Guarner J, Paddock CD, Rota P, Fields B, DeRisi J, Yang JY, Cox N, Hughes JM, LeDuc JW, Bellini WJ, Anderson LJ; SARS Working Group. 2003. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med 348: 1953– 1966.
29. Lipson SM, Walderman R, Costello P, Szabo K. 1988. Sensitivity of rhabdomyosarcoma and guinea pig embryo cell cultures to field isolates of difficult-to-cultivate group A coxsackieviruses. J Clin Microbiol 26: 1298– 1303.
30. Schmidt NJ, Ho HH, Lennette EH. 1975. Propagation and isolation of group A coxsackieviruses in RD cells. J Clin Microbiol 2: 183– 185.
31. Stabell EC, O’Rourke SR, Storch GA, Olivo PD. 1993. Evaluation of a genetically engineered cell line and a histochemical β-galactosidase assay to detect herpes simplex virus in clinical specimens. J Clin Microbiol 31: 2796– 2798.
32. Tobita K, Sugiura A, Enomoto C, Furuyama M. 1975. Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Med Microbiol Immunol 162: 9– 14.
33. Boivin G, Abed Y, Pelletier G, Ruel L, Moisan D, Cote S, Peret TCT, Erdman DD, Anderson LJ. 2002. Virological features and clinical manifestations associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratory-tract infections in all age groups. J Infect Dis 186: 1330– 1334.
34. Hamelin ME, Boivin G. 2005. Human metapneumovirus: a ubiquitous and long-standing respiratory pathogen. Pediatr Infect Dis J 24: S203– S207.
35. Van den Hoogen BG, de Jong JC, Groen J, Kuiken T, de Groot R, Fouchier RA, Osterhaus AD. 2001. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med 7: 719– 724.
36. Peret TC, Boivin G, Li Y, Couillard M, Humphrey C, Osterhaus AD, Erdman DD, Anderson LJ. 2002. Characterization of human metapneumoviruses isolated from patients in North America. J Infect Dis 185: 1660– 1663.
37. Rogers J, Rohal S, Carrigan D, Kusne S, Knox K, Gayowski T, Wagener M, Fung JJ, Singh N. 2000. Human herpesvirus 6 in liver transplant recipients: role in pathogenesis of fungal infections, neurologic complications, and outcome. Transplantation 69: 2566– 2573.
1. Frank AL, Couch RB, Griffis CA, Baxter BD. 1979. Comparison of different tissue cultures for isolation and quantitation of influenza and parainfluenza viruses. J Clin Microbiol 10: 32– 36.
2. Tobita K, Sugiura A, Enomoto C, Furuyama M. 1975. Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Med Microbiol Immunol 162: 9– 14.

Tables

Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures
Citation: Leber A. 2016. Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures, p 10.2.1-10.2.11. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch10.2
/content/10.1128/9781555818814.chap10.2.table10.2-1
Table 10.2–1
Monolayer cell cultures used for viral culture
Generic image for table
Table 10.2–1

Monolayer cell cultures used for viral culture

Citation: Leber A. 2016. Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures, p 10.2.1-10.2.11. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch10.2
/content/10.1128/9781555818814.chap10.2.table10.2-2
Table 10.2–2
Viral culture systems and availability
Generic image for table
Table 10.2–2

Viral culture systems and availability

Citation: Leber A. 2016. Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures, p 10.2.1-10.2.11. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch10.2
/content/10.1128/9781555818814.chap10.2.table10.2-3
Table 10.2–3
Assessment of monolayer cell cultures and troubleshooting
Generic image for table
Table 10.2–3

Assessment of monolayer cell cultures and troubleshooting

Citation: Leber A. 2016. Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures, p 10.2.1-10.2.11. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch10.2

Back to top
This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error