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Category: Clinical Microbiology
Selection, Maintenance, and Observation of Uninoculated Monolayer Cell Cultures, Page 1 of 2
< Previous page | Next page > /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap10.2-1.gif /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap10.2-2.gifAbstract:
Viruses may contain genetic information for many functions in addition to viral structural protein genes, notably those associated with replication and immune system modulation. However, even viruses with very large genomes and extensive viral-coded functions are all obligate intracellular parasites, requiring metabolically active cells to support their replication. Many human pathogenic viruses can be cultured in readily available cell culture monolayers ( Table 10.2–1 ). However, some can be isolated only by using specialized systems ( Table 10.2–2 ) such as organ culture, suspension leukocyte culture, or animals. Additionally, there are several agents for which no in vitro system has been identified.
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Assessment, incubation, and maintenance of uninoculated cell cultures.
Focal area of replicating A-549 cells that have cross-contaminated an MRC-5 monolayer.
Monolayer cell cultures used for viral culture
Assessment of monolayer cell cultures and troubleshooting