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Cell Culture Techniques: Serial Propagation and Maintenance of Monolayer Cell Cultures, Page 1 of 2
< Previous page Next page > /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap10.3-1.gif /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap10.3-2.gifAbstract:
The majority of diagnostic laboratories that perform virus culture obtain the requisite cells from a commercial vendor, already seeded in the vessels that will be inoculated. Alternatively, they receive them in a near-ready state, with minimal manipulations required before the cultures are ready to inoculate. Few laboratories still maintain in-house seed stocks and routinely culture, passage, and prepare cultures for virus culture work. For those that choose to, it is imperative that they have the appropriate facilities and adhere strictly to detailed, well-established protocols, to ensure an uninterrupted supply of healthy cell cultures for the diagnostic laboratory. The procedure detailed below provides information on these procedures and is revised from the previously published edition ( 1 ).