Chapter 11.18 : Lymphocyte Proliferation Assay

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Measuring the proliferative capability of lymphocytes is important in the evaluation of an individual’s immune system. This can be achieved in an assay which cultures lymphocytes with either a mitogen or an antigen that can stimulate a proliferative response and then labels the cells with a radioactive marker to allow for measurement of response ( ).

Citation: Leber A. 2016. Lymphocyte Proliferation Assay, p 11.18.1-11.18.5. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch11.18
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1. Fletcher MA, Klimas N, Morgan R, Gjerset G. 1992. Lymphocyte proliferation, p 213–219. In Rose NR, Conway de Macario E, Fahey JL, Friedman H, Penn GM (ed), Manual of Clinical Laboratory Immunology, 4th ed. American Society for Microbiology, Washington, DC.
2. Froebel KS, Pakker NG, Aiuti F, Bofill M, Choremi-Papadopoulou H, Economidou J, Rabian C, Roos MTL, Ryder LP, Miedema F. 1999. Standardization and quality assurance of lymphocyte proliferation assays for use in the assessment of immune function. J Immunol Methods 227: 8597.

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