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Chapter 11.21 : Bead- and Plate-Based Cytokine Enzyme-Linked Immunosorbent Assays

Editor: Amy L. Leber1
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Affiliations: 1: Editor in chief, Department of Laboratory Medicine, Nationwide Children’s Hospital, Columbus, Ohio
Content Type: Reference
Publication Year: 2016

Category: Clinical Microbiology

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Citation: Leber A. 2016. Bead- and Plate-Based Cytokine Enzyme-Linked Immunosorbent Assays, p 11.21.1.1-11.21.2.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch11.21
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Bead- and Plate-Based Cytokine Enzyme-Linked Immunosorbent Assays
Citation: Leber A. 2016. Bead- and Plate-Based Cytokine Enzyme-Linked Immunosorbent Assays, p 11.21.1.1-11.21.2.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch11.21
/content/10.1128/9781555818814.chap11.21.fig11.21.1-1
Figure 11.21.1–1
Bead-based ELISA methods allow for the analysis of multiple analytes with a single sample. Microspheres are conjugated to the desired capture antibody that detects the analyte of interest. A second sandwich biotinylated antibody is subsequently used for detection following addition of fluorochrome-conjugated streptavidin.
10.1128/9781555818814/fig11.21.1-1_thmb.gif
10.1128/9781555818814/fig11.21.1-1.gif
Image of Figure 11.21.1–1
Figure 11.21.1–1

Bead-based ELISA methods allow for the analysis of multiple analytes with a single sample. Microspheres are conjugated to the desired capture antibody that detects the analyte of interest. A second sandwich biotinylated antibody is subsequently used for detection following addition of fluorochrome-conjugated streptavidin.

Citation: Leber A. 2016. Bead- and Plate-Based Cytokine Enzyme-Linked Immunosorbent Assays, p 11.21.1.1-11.21.2.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch11.21
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/content/10.1128/9781555818814.chap11.21.fig11.21.1-2
Figure 11.21.1–2
Layout for 96-well plate. This set-up allows for multiple simultaneous measurements and up to 40 test samples in duplicate. The number of analytes depends on the kit.
10.1128/9781555818814/fig11.21.1-2_thmb.gif
10.1128/9781555818814/fig11.21.1-2.gif
Image of Figure 11.21.1–2
Figure 11.21.1–2

Layout for 96-well plate. This set-up allows for multiple simultaneous measurements and up to 40 test samples in duplicate. The number of analytes depends on the kit.

Citation: Leber A. 2016. Bead- and Plate-Based Cytokine Enzyme-Linked Immunosorbent Assays, p 11.21.1.1-11.21.2.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch11.21
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References

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1. Wong HL, Pfeiffer RM, Fears TR, Vermeulen R, Ji S, Rabkin CS. 2008. Reproducibility and correlations of multiplex cytokine levels in asymptomatic persons. Cancer Epidemiol Biomarkers Prev 17: 3450– 3456.
2. Leng SX, McElhaney JE, Walston JD, Xie D, Fedarko NS, Kuchel GA. 2008. ELISA and multiplex technologies for cytokine measurements in inflammation and aging research. J Gerantol A Bio Sci Med 8: 879– 884.

Tables

Bead- and Plate-Based Cytokine Enzyme-Linked Immunosorbent Assays
Citation: Leber A. 2016. Bead- and Plate-Based Cytokine Enzyme-Linked Immunosorbent Assays, p 11.21.1.1-11.21.2.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch11.21
/content/10.1128/9781555818814.chap11.21.table11.21.1-1
Table 11.21.1–1
Substrates
Generic image for table
Table 11.21.1–1

Substrates

Citation: Leber A. 2016. Bead- and Plate-Based Cytokine Enzyme-Linked Immunosorbent Assays, p 11.21.1.1-11.21.2.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch11.21

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