Chapter 3.14 : Culture

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Leptospirosis is a zoonosis caused by spirochetes of the genus . Pathogenic leptospires are maintained in nature by chronic renal infection of carrier mammals, which excrete the organism in their urine ( ). Human infection is acquired by contact of mucous membranes or broken skin with infected animal tissues or body fluids or with water, damp soil, or sewage contaminated with the urine of infected animals ( ). The genus was previously divided into two species, comprising all pathogenic strains, and , containing saprophytic strains isolated from water; the two species were differentiated by a number of phenotypic tests ( ).

Citation: Levett P. 2016. Culture, p 3.14.1-3.14.5. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch3.14
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12. Ellinghausen HC, McCullough WG. 1965. Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am J Vet Res 26: 4551.
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15. Adler B, Faine S. 2006. The genus Leptospira, p 294317. In Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E (ed), The Prokaryotes, 3rd ed, vol 7. Springer, New York, NY.
16. Vijayachari P, Sugunan AP, Umapathi T, Sehgal SC. 2001. Evaluation of darkground microscopy as a rapid diagnostic procedure in leptospirosis. Indian J Med Res 114: 5458.
17. Johnson RC, Rogers P. 1964. 5-Fluorouracil as a selective agent for growth of leptospirae. J Bacteriol 87: 422426.
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20. Galloway RL, Levett PN. 2010. Application and validation of PFGE for serovar identification of Leptospira clinical isolates. PLoS Negl Trop Dis 4: e824.

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