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Chapter 3.17 : Biochemical Tests for the Identification of Aerobic Bacteria

Author: Deirdre L. Church
Content Type: Reference
Publication Year: 2016

Category: Clinical Microbiology

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Biochemical Tests for the Identification of Aerobic Bacteria, Page 1 of 2

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Abstract:

Acetamide agar is used to test an organism’s ability to utilize acetamide by deamidation. The medium contains acetamide as the sole carbon source and inorganic ammonium salts as the sole source of nitrogen. Growth is indicative of a positive test for acetamide utilization. When the bacterium metabolizes acetamide by the enzymatic action of an acylamidase, the ammonium salts are broken down to ammonia, which increases alkalinity. The shift in pH turns the bromthymol blue indicator in the medium from green to blue, indicative of a positive test. Assimilation of acetamide will result in a yellow color and should not be mistaken for a positive result ( ). In general, deamidation is limited to only a few organisms. This medium is recommended for differentiating from other non-glucose-fermenting, Gram-negative rods ( ).

Citation: Church D. 2016. Biochemical Tests for the Identification of Aerobic Bacteria, p 3.17.1.1-3.17.48.3. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch3.17
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Biochemical Tests for the Identification of Aerobic Bacteria
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Citation: Church D. 2016. Biochemical Tests for the Identification of Aerobic Bacteria, p 3.17.1.1-3.17.48.3. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch3.17
/content/10.1128/9781555818814.chap3.17.fig3.17.8-1
Figure 3.17.8–1
Demonstration of positive CAMP and reverse CAMP test, using streak and CAMP disk.
10.1128/9781555818814/fig3.17.8-1_thmb.gif
10.1128/9781555818814/fig3.17.8-1.gif
Image of Figure 3.17.8–1
Figure 3.17.8–1

Demonstration of positive CAMP and reverse CAMP test, using streak and CAMP disk.

Citation: Church D. 2016. Biochemical Tests for the Identification of Aerobic Bacteria, p 3.17.1.1-3.17.48.3. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch3.17
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References

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Tables

Biochemical Tests for the Identification of Aerobic Bacteria
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Citation: Church D. 2016. Biochemical Tests for the Identification of Aerobic Bacteria, p 3.17.1.1-3.17.48.3. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch3.17
/content/10.1128/9781555818814.chap3.17.table0026
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Citation: Church D. 2016. Biochemical Tests for the Identification of Aerobic Bacteria, p 3.17.1.1-3.17.48.3. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch3.17
/content/10.1128/9781555818814.chap3.17.table0027
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Citation: Church D. 2016. Biochemical Tests for the Identification of Aerobic Bacteria, p 3.17.1.1-3.17.48.3. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch3.17
/content/10.1128/9781555818814.chap3.17.table0028
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Citation: Church D. 2016. Biochemical Tests for the Identification of Aerobic Bacteria, p 3.17.1.1-3.17.48.3. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch3.17
/content/10.1128/9781555818814.chap3.17.table0029
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Generic image for table
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Citation: Church D. 2016. Biochemical Tests for the Identification of Aerobic Bacteria, p 3.17.1.1-3.17.48.3. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch3.17
/content/10.1128/9781555818814.chap3.17.table0030
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Generic image for table
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Citation: Church D. 2016. Biochemical Tests for the Identification of Aerobic Bacteria, p 3.17.1.1-3.17.48.3. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch3.17

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