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Category: Clinical Microbiology
Biochemical Tests for the Identification of Aerobic Bacteria, Page 1 of 2
< Previous page | Next page > /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap3.17-1.gif /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap3.17-2.gifAbstract:
Acetamide agar is used to test an organism’s ability to utilize acetamide by deamidation. The medium contains acetamide as the sole carbon source and inorganic ammonium salts as the sole source of nitrogen. Growth is indicative of a positive test for acetamide utilization. When the bacterium metabolizes acetamide by the enzymatic action of an acylamidase, the ammonium salts are broken down to ammonia, which increases alkalinity. The shift in pH turns the bromthymol blue indicator in the medium from green to blue, indicative of a positive test. Assimilation of acetamide will result in a yellow color and should not be mistaken for a positive result ( 1 ). In general, deamidation is limited to only a few organisms. This medium is recommended for differentiating Pseudomonas aeruginosa from other non-glucose-fermenting, Gram-negative rods ( 2 , 3 ).
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Demonstration of positive CAMP and reverse CAMP test, using S. aureus streak and CAMP disk.