
Full text loading...
Category: Clinical Microbiology
5.14 Tests To Assess Bactericidal Activity, Page 1 of 2
< Previous page | Next page > /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap5.14-1.gif /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap5.14-2.gifAbstract:
The minimum bactericidal concentration (MBC) test can be used to assess the ability of an antimicrobial agent to kill a bacterial isolate. MBC tests are performed after consultation in very select clinical situations when it is necessary to determine the bactericidal activity of an antimicrobial agent against a bacterial isolate. These situations include those in which immune mechanisms offer little help in eradicating the infecting organisms, such as in patients with endocarditis or osteomyelitis or in immunosuppressed patients, particularly those with neutropenia. Bactericidal tests provide a rough prediction of bacterial eradication, but other factors may also impact the bacteriologic responses of patients. MBC testing is an accepted parameter in the evaluation of new antimicrobial agents and is frequently used as a research tool.
Full text loading...
MIC/MBC QC
MBC Worksheet
Dilution scheme for bacterial colony counts.
Plate configuration for inoculating 0.025 ml of each dilution from reaction tube to agar plate for colony counts. (Adapted and reprinted with permission from Pillai SK, Moellering Jr RC Jr, Eliopoulos GM. 2005. Antimicrobial combinations, p 365–440, Fig. 9.4 [p 373]. In Lorian V [ed], Antibiotics in Laboratory Medicine, 5th ed. The Williams & Wilkins Co, New York, NY. doi:10.1128/9781555818814.ch5.14.2.fA2
Time-Kill Assay Worksheet
Time-Kill Assay Worksheet
MBC testing conditions for various bacteria for microdilution and macrodilution procedures a
Rejection value and calculated sensitivity and specificity for each initial concentration based on duplicate 0.01-ml samples a
MBC testing conditions for various bacteria for microdilution and macrodilution procedures a