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Chapter 5.3 : Etest

Editor: Amy L. Leber1
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Affiliations: 1: Editor in chief, Department of Laboratory Medicine, Nationwide Children’s Hospital, Columbus, Ohio
Content Type: Reference
Publication Year: 2016

Category: Clinical Microbiology

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Etest, Page 1 of 2

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Abstract:

A standardized inoculum of bacteria is swabbed onto the surface of a Mueller-Hinton agar (MHA) plate. Etest strips containing a continuous gradient of antimicrobial concentrations are placed on the agar surface. After overnight incubation, an elliptical zone of inhibition forms as the antimicrobial agent inhibits growth. The MIC is read where growth intersects the

Citation: Leber A. 2016. Etest, p 5.3.1-5.3.9. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.3
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References

/content/book/10.1128/9781555818814.chap5.3
1. CLSI. 2013. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. M100-S23. CLSI, Wayne, PA.
2. CLSI. 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 9th ed. Approved standard M07-A9. CLSI, Wayne, PA.
3. Novak S, Munro S. 2010. Etest. In Garcia LS (ed), Clinical Microbiology Procedures Handbook, 3rd ed. ASM Press, Washington, DC.
4. Baker CN, Stocker SA, Culver DH, Thorns-berry C. 1991. Comparison of the Etest to agar dilution, broth microdilution, and agar diffusion susceptibility testing techniques by using a special challenge set of bacteria. J Clin Microbiol 29: 533– 538.
5. CLSI. 2010. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria, 2nd ed. Approved guideline M45-A2. CLSI, Wayne, PA.
6. Goldstein EJ, Citron DM, Goldman PJ, Goldman RJ. 2008. National hospital survey of anaerobic culture and susceptibility methods: III. Anaerobe 14( 2): 68– 72.
7. Hindler JA, Jorgensen JH. 2011. Susceptibility test methods: fastidious bacteria, p 1180–1203. In Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW (ed), Manual of Clinical Microbiology, 10th ed. ASM Press, Washington, DC.
8. Patel JB, Tenover FC, Turnidge JD, Jorgensen JH. 2011. Susceptibility test methods: dilution and disk diffusion methods, p 1122–1143. In Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW (ed), Manual of Clinical Microbiology, 10th ed. ASM Press, Washington, DC.
9. Winstanley T, Courvalin P. 2011. Expert systems in clinical microbiology. Clin Microbiol Rev 24: 515– 555.

Tables

Etest
Citation: Leber A. 2016. Etest, p 5.3.1-5.3.9. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.3
/content/10.1128/9781555818814.chap5.3.table0047
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Untitled

Citation: Leber A. 2016. Etest, p 5.3.1-5.3.9. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.3
/content/10.1128/9781555818814.chap5.3.table0048
Quick reference list for performing Etesta
Quick reference list for performing Etest
Generic image for table
Quick reference list for performing Etesta

Quick reference list for performing Etest

Citation: Leber A. 2016. Etest, p 5.3.1-5.3.9. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.3
/content/10.1128/9781555818814.chap5.3.table0049
Etest QC
Etest QC
Generic image for table
Etest QC

Etest QC

Citation: Leber A. 2016. Etest, p 5.3.1-5.3.9. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.3

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