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Chapter 8.7 : Identification of Moulds on Primary Culture

Editor: Amy L. Leber1
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Affiliations: 1: Editor in chief, Department of Laboratory Medicine, Nationwide Children’s Hospital, Columbus, Ohio
Content Type: Reference
Publication Year: 2016

Category: Clinical Microbiology

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Identification of Moulds on Primary Culture, Page 1 of 2

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Abstract:

A pure culture is a prerequisite for the identification of a fungus. Fungi may be polymorphic, and any visible structures present must be demonstrated to be produced by one fungus, not several. Bacteria may also interfere with the development of key characteristics.

Citation: Leber A. 2016. Identification of Moulds on Primary Culture, p 8.7.1-8.7.8. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.7
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References

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1. St. Germain G, R. Summerbell 2010. Identifying Filamentous Fungi, 2nd ed. Star Publishing Co., Belmont, CA.
2. Beneke ES, AL. Rogers 1996. Medical Mycology and Human Mycoses. Star Publishing Co., Belmont, CA.
3. Ellis D, Davis S, Alexiou H, Handke R, R. Bartley 2007. Descriptions of Medical Fungi, 2nd ed. Adelaide Medical Centre for Women and Children, North Adelaide, Australia.
4. Campbell CK, Johnson EM, DW. Warnock 2013. Identification of Pathogenic Fungi, 2nd ed. Wiley–Blackwell, Hoboken, NJ.
5. L’Ollivier C, Cassagne C, Normand AC, Bouchara JP, Contet-Audonneau N, Hendrickx M, Fourquet P, Coulibaly O, Piarroux R, Ranque S. 2013. A MALDI-TOF MS procedure for clinical dermatophyte species identification in the routine laboratory. Med Mycol 51: 713– 720.
6. Ranque S, Normand AC, Cassagne C, Murat JB, Bourgeois N, Dalle F, Gari-Toussaint M, Fourquet P, Hendrickx M, R. Piarroux 2014. MALDI-TOF mass spectrometry identification of filamentous fungi in the clinical laboratory. Mycoses 57: 135– 140.
7. Singh PK, Kathuria S, Agarwal K, Gaur SN, Meis JF, A. Chowdhary 2013. Clinical significance and molecular characterization of nonsporulating molds isolated from the respiratory tracts of bronchopulmonary mycosis patients with special reference to basidiomycetes. J Clin Microbiol 51: 3331– 3337.
8. Li HC, Bouchara JP, Hsu MM, Barton R, Su S, TC. Chang 2008. Identification of dermatophytes by sequence analysis of the rRNA gene internal transcribed spacer regions. J Med Microbiol 57( Pt 5): 592- 600.
9. Verrier J, Krähenbühl L, Bontems O, Fratti M, Salamin K, Monod M 2013. Dermatophyte identification in skin and hair samples using a simple and reliable nested polymerase chain reaction assay. Br J Dermatol 168: 295– 301.

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