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Chapter 8.8 : Full Identification of Yeasts

Editor: Amy L. Leber1
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Affiliations: 1: Editor in chief, Department of Laboratory Medicine, Nationwide Children’s Hospital, Columbus, Ohio
Content Type: Reference
Publication Year: 2016

Category: Clinical Microbiology

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Abstract:

In an era of increasing resistance of yeast species to antifungal agents and a widening range of species capable of causing diseases previously the domain of there is almost no situation in which identification to species level is not warranted. This is especially true given the growth in the number of immunocompromised patients in our society, which has provided more opportunities for yeast infections to occur and to complicate and prolong the recovery period. Molecular methods to identify yeasts directly in specimens and after growth in culture are under development. Peptide nucleic acid fluorescence hybridization (PNA FISH) technology (AdvanDx, Woburn, MA) allows identification of several yeasts directly from blood cultures. Recently, matrix-assisted laser desorption ionization–time of flight mass spectroscopy (MALDI-TOF MS) has been introduced as a method with the potential to identify a wide range of bacteria and fungi. Several research groups have used MALDI-TOF MS to identify a variety of clinically important yeasts ( ). The currently FDA-approved IVD database for the Vitek MS (bioMérieux, Durham, NC) lists 68 yeast species encompassing 12 genera (including 37 species of ).

Citation: Leber A. 2016. Full Identification of Yeasts, p 8.8.1-8.8.14. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.8
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Tables

Full Identification of Yeasts
Citation: Leber A. 2016. Full Identification of Yeasts, p 8.8.1-8.8.14. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.8
/content/10.1128/9781555818814.chap8.8.table8.8-1
Table 8.8–1
Anamorph-teleomorph binomials of commonly encountered yeasts
Generic image for table
Table 8.8–1

Anamorph-teleomorph binomials of commonly encountered yeasts

Citation: Leber A. 2016. Full Identification of Yeasts, p 8.8.1-8.8.14. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.8
/content/10.1128/9781555818814.chap8.8.table8.8-2
Table 8.8–2
General considerations of two commercial yeast identification systems
Generic image for table
Table 8.8–2

General considerations of two commercial yeast identification systems

Citation: Leber A. 2016. Full Identification of Yeasts, p 8.8.1-8.8.14. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.8
/content/10.1128/9781555818814.chap8.8.table8.8-3
Table 8.8–3
Examples of useful supplemental tests for yeasts
Generic image for table
Table 8.8–3

Examples of useful supplemental tests for yeasts

Citation: Leber A. 2016. Full Identification of Yeasts, p 8.8.1-8.8.14. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.8
/content/10.1128/9781555818814.chap8.8.table8.8-4
Table 8.8–4
Culture and biochemical characteristics of yeasts frequently isolated from clinical specimens
Generic image for table
Table 8.8–4

Culture and biochemical characteristics of yeasts frequently isolated from clinical specimens

Citation: Leber A. 2016. Full Identification of Yeasts, p 8.8.1-8.8.14. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.8
/content/10.1128/9781555818814.chap8.8.table8.8-5
Table 8.8–5
Characteristics of selected spp.
Generic image for table
Table 8.8–5

Characteristics of selected spp.

Citation: Leber A. 2016. Full Identification of Yeasts, p 8.8.1-8.8.14. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.8
/content/10.1128/9781555818814.chap8.8.table8.8-6
Table 8.8–6
Fermentation reactions for some spp.
Generic image for table
Table 8.8–6

Fermentation reactions for some spp.

Citation: Leber A. 2016. Full Identification of Yeasts, p 8.8.1-8.8.14. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.8
/content/10.1128/9781555818814.chap8.8.table8.8-7
Table 8.8–7
Key characteristics to differentiate species
Generic image for table
Table 8.8–7

Key characteristics to differentiate species

Citation: Leber A. 2016. Full Identification of Yeasts, p 8.8.1-8.8.14. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.8

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