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Chapter 9.8 : D B P

Editor: Amy L. Leber1
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Affiliations: 1: Editor in chief, Department of Laboratory Medicine, Nationwide Children’s Hospital, Columbus, Ohio
Content Type: Reference
Publication Year: 2016

Category: Clinical Microbiology

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Abstract:

During some stages in their life cycle, spp. (malaria), spp., spp., , and the filariae are detectable in human blood ( ). and species are found within the RBCs; trypanosomes and microfilariae, the larval stage of filariae, are found outside the RBCs; and amastigotes are occasionally found within monocytes. Trypanosomes and microfilariae, which frequently are present in low numbers, exhibit motility in freshly collected blood films, and this can aid in their detection. However, species identifications of all blood parasites are usually made from either or both of two types of stained blood films: a thin film and a thick film. These films can be made from whole or anticoagulated blood or from the sediment of a variety of procedures designed to concentrate trypanosomes and microfilariae in the blood. Although the films have traditionally been stained with Giemsa stain, many infections are detected and diagnosed by using Wright’s stain or other blood stains, including the rapid stains. Delafield’s hematoxylin is used to enhance the morphological features of microfilariae.

Citation: Leber A. 2016. D B P, p 9.8.1.1-9.8.13.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.8
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Detection of Blood Parasites
Citation: Leber A. 2016. D B P, p 9.8.1.1-9.8.13.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.8
/content/10.1128/9781555818814.chap9.8.fig9.8.4-1
Figure 9.8.4–1
Method of thick-thin combination blood film preparation. (a) Position of drop of EDTA blood; (b) position of applicator stick in contact with blood and glass slide; (c) rotation of applicator stick; (d) completed thick-thin combination blood film prior to staining. (Illustration by Sharon Belkin; from reference .)
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Image of Figure 9.8.4–1
Figure 9.8.4–1

Method of thick-thin combination blood film preparation. (a) Position of drop of EDTA blood; (b) position of applicator stick in contact with blood and glass slide; (c) rotation of applicator stick; (d) completed thick-thin combination blood film prior to staining. (Illustration by Sharon Belkin; from reference .)

Citation: Leber A. 2016. D B P, p 9.8.1.1-9.8.13.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.8
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Figure 9.8.13–1
BinaxNOW malaria device for result interpretation. Courtesy of Alere (http://www.alere.com/us/en/product-details/binaxnow-malaria.html).
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Figure 9.8.13–1

BinaxNOW malaria device for result interpretation. Courtesy of Alere (http://www.alere.com/us/en/product-details/binaxnow-malaria.html).

Citation: Leber A. 2016. D B P, p 9.8.1.1-9.8.13.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.8
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Tables

Detection of Blood Parasites
Citation: Leber A. 2016. D B P, p 9.8.1.1-9.8.13.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.8
/content/10.1128/9781555818814.chap9.8.table0133
Advantages of thick and thin blood films
Advantages of thick and thin blood films
Generic image for table
Advantages of thick and thin blood films

Advantages of thick and thin blood films

Citation: Leber A. 2016. D B P, p 9.8.1.1-9.8.13.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.8
/content/10.1128/9781555818814.chap9.8.table0134
Potential problems using EDTA anticoagulant for the preparation of thin and thick blood films
Potential problems using EDTA anticoagulant for the preparation of thin and thick blood films
Generic image for table
Potential problems using EDTA anticoagulant for the preparation of thin and thick blood films

Potential problems using EDTA anticoagulant for the preparation of thin and thick blood films

Citation: Leber A. 2016. D B P, p 9.8.1.1-9.8.13.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.8
/content/10.1128/9781555818814.chap9.8.table9.8.7-1
Table 9.8.7–1
Parasitemia determined from conventional light microscopy: clinical correlation
Generic image for table
Table 9.8.7–1

Parasitemia determined from conventional light microscopy: clinical correlation

Citation: Leber A. 2016. D B P, p 9.8.1.1-9.8.13.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.8
/content/10.1128/9781555818814.chap9.8.table9.8.7-2
Table 9.8.7–2
Malaria resistance
Generic image for table
Table 9.8.7–2

Malaria resistance

Citation: Leber A. 2016. D B P, p 9.8.1.1-9.8.13.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.8
/content/10.1128/9781555818814.chap9.8.table0135
Advantages and disadvantages of buffy coat films
Advantages and disadvantages of buffy coat films
Generic image for table
Advantages and disadvantages of buffy coat films

Advantages and disadvantages of buffy coat films

Citation: Leber A. 2016. D B P, p 9.8.1.1-9.8.13.3. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.8

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